Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management
ABSTRACT Understanding the diversity and ecological evolutionary history of plant species is crucial for addressing the current biodiversity crisis and comprehending the processes by which organisms fill ecological and geographic spaces. In this study, we present a comprehensive analysis of the dive...
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| Format: | Article |
| Language: | English |
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Wiley
2025-03-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.71079 |
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| author | Marcin Nowicki Logan C. Houston Sarah L. Boggess Matthew L. Huff Margaret E. Staton Robert N. Trigiano |
| author_facet | Marcin Nowicki Logan C. Houston Sarah L. Boggess Matthew L. Huff Margaret E. Staton Robert N. Trigiano |
| author_sort | Marcin Nowicki |
| collection | DOAJ |
| description | ABSTRACT Understanding the diversity and ecological evolutionary history of plant species is crucial for addressing the current biodiversity crisis and comprehending the processes by which organisms fill ecological and geographic spaces. In this study, we present a comprehensive analysis of the diversity and evolutionary history of Diphasiastrum digitatum Holub from the lycophyte lineage of plants, using microsatellite genotyping data and biogeographic analyses. Based on the available transcriptome assembly, we generated numerous markers and utilized 13 robust microsatellite markers to genotype a collection of 402 specimens from the Eastern US (VT; VA; NC; TN). In accordance with the accepted phylogeny, cross‐amplification tests demonstrated a closer relationship between D. digitatum and Diphasiastrum spp. compared with Lycopodium spp. Furthermore, the population genetics analyses identified two genetic clusters within the D. digitatum collection and suggested ongoing divergence and expansion. Isolation‐by‐distance analysis indicated that geographic distance had a minimal effect on differentiation, whereas environmental variables related to water regime were strongly associated with the genetic variance. Ecological niche modeling showed a post‐Last Glacial Maximum expansion of D. digitatum from southern refugia, corroborating a similar evolutionary scenario based on our microsatellite data. Overall, this study provides valuable insights into the evolutionary history of clubmosses and highlights the migration events and the environmental factors that shaped their current distribution. |
| format | Article |
| id | doaj-art-a85bc3ba97bc4b82ac9cdcd6d9d481bc |
| institution | Kabale University |
| issn | 2045-7758 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj-art-a85bc3ba97bc4b82ac9cdcd6d9d481bc2025-08-20T03:52:25ZengWileyEcology and Evolution2045-77582025-03-01153n/an/a10.1002/ece3.71079Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem ManagementMarcin Nowicki0Logan C. Houston1Sarah L. Boggess2Matthew L. Huff3Margaret E. Staton4Robert N. Trigiano5Entomology and Plant Pathology University of Tennessee Knoxville Tennessee USAEntomology and Plant Pathology University of Tennessee Knoxville Tennessee USAEntomology and Plant Pathology University of Tennessee Knoxville Tennessee USAEntomology and Plant Pathology University of Tennessee Knoxville Tennessee USAEntomology and Plant Pathology University of Tennessee Knoxville Tennessee USAEntomology and Plant Pathology University of Tennessee Knoxville Tennessee USAABSTRACT Understanding the diversity and ecological evolutionary history of plant species is crucial for addressing the current biodiversity crisis and comprehending the processes by which organisms fill ecological and geographic spaces. In this study, we present a comprehensive analysis of the diversity and evolutionary history of Diphasiastrum digitatum Holub from the lycophyte lineage of plants, using microsatellite genotyping data and biogeographic analyses. Based on the available transcriptome assembly, we generated numerous markers and utilized 13 robust microsatellite markers to genotype a collection of 402 specimens from the Eastern US (VT; VA; NC; TN). In accordance with the accepted phylogeny, cross‐amplification tests demonstrated a closer relationship between D. digitatum and Diphasiastrum spp. compared with Lycopodium spp. Furthermore, the population genetics analyses identified two genetic clusters within the D. digitatum collection and suggested ongoing divergence and expansion. Isolation‐by‐distance analysis indicated that geographic distance had a minimal effect on differentiation, whereas environmental variables related to water regime were strongly associated with the genetic variance. Ecological niche modeling showed a post‐Last Glacial Maximum expansion of D. digitatum from southern refugia, corroborating a similar evolutionary scenario based on our microsatellite data. Overall, this study provides valuable insights into the evolutionary history of clubmosses and highlights the migration events and the environmental factors that shaped their current distribution.https://doi.org/10.1002/ece3.71079ecological niche modelingevolutionary dynamicsfan clubmossphylogeographySouthern ground cedarspecies management |
| spellingShingle | Marcin Nowicki Logan C. Houston Sarah L. Boggess Matthew L. Huff Margaret E. Staton Robert N. Trigiano Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management Ecology and Evolution ecological niche modeling evolutionary dynamics fan clubmoss phylogeography Southern ground cedar species management |
| title | Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management |
| title_full | Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management |
| title_fullStr | Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management |
| title_full_unstemmed | Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management |
| title_short | Integrative Analysis of Diphasiastrum digitatum Holub: Unveiling Genetic Variation and Ecological Adaptations for Sustainable Ecosystem Management |
| title_sort | integrative analysis of diphasiastrum digitatum holub unveiling genetic variation and ecological adaptations for sustainable ecosystem management |
| topic | ecological niche modeling evolutionary dynamics fan clubmoss phylogeography Southern ground cedar species management |
| url | https://doi.org/10.1002/ece3.71079 |
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