Texture Analysis of Near-Infrared Vein Images During Reactive Hyperemia in Healthy Subjects

Venous perfusion plays a crucial role in vascular health, yet functional assessment of superficial veins remains limited. Near-infrared reflectance imaging (NIRI) devices, commonly used for vein visualization, may offer untapped potential in this context. We investigated whether texture analysis (TA...

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Bibliographic Details
Main Authors: Henrique Silva, Carlota Rezendes
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Applied Sciences
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Online Access:https://www.mdpi.com/2076-3417/15/10/5702
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Summary:Venous perfusion plays a crucial role in vascular health, yet functional assessment of superficial veins remains limited. Near-infrared reflectance imaging (NIRI) devices, commonly used for vein visualization, may offer untapped potential in this context. We investigated whether texture analysis (TA) applied to NIRI-based vein finder images can detect dynamic changes in superficial venous structure during reactive hyperemia. Fourteen healthy adults underwent a suprasystolic occlusion protocol, with real-time images acquired from the hand dorsum. From defined regions of interest, we extracted classical texture parameters (e.g., contrast, correlation, entropy, energy, fractal dimension, and lacunarity) and vein width. While vein width significantly increased during occlusion (<i>p</i> < 0.001), most individual texture parameters remained stable. Notably, correlation increased during occlusion (<i>p</i> = 0.023), and lacunarity decreased during recovery (<i>p</i> = 0.024). We developed composite indices combining texture and morphological features. Entropy-to-width and correlation-to-width ratios decreased during occlusion (<i>p</i> < 0.001), while total entropic content rose (<i>p</i> < 0.001). A modest increase in the correlation-to-entropy ratio during recovery (<i>p</i> = 0.026) suggested delayed reorganization of venous texture. These findings indicate that TA of vein finder images captures functional vascular responses beyond morphology alone. Composite parameters enhance sensitivity and may support the development of non-invasive, low-cost tools for assessing venous function.
ISSN:2076-3417