Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation
Ischemic stroke is a leading cause of global death. The treatment of this disease can inevitably result in reperfusion, thereby triggering cerebral ischemia-reperfusion injury (IRI) and neuronal pyroptosis. Electroacupuncture derived from traditional acupuncture has been proven to have favorable eff...
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Elsevier
2025-01-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923024003071 |
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| author | Lili Ma Meiling Zhang Ting Chen Limin Wang Qilong Deng |
| author_facet | Lili Ma Meiling Zhang Ting Chen Limin Wang Qilong Deng |
| author_sort | Lili Ma |
| collection | DOAJ |
| description | Ischemic stroke is a leading cause of global death. The treatment of this disease can inevitably result in reperfusion, thereby triggering cerebral ischemia-reperfusion injury (IRI) and neuronal pyroptosis. Electroacupuncture derived from traditional acupuncture has been proven to have favorable effects on ameliorating brain IRI and pyroptosis. Hence, the goal of the current research was to elucidate the mechanism governing electroacupuncture in cerebral IRI. We employed middle cerebral artery occlusion (MCAO) model to induce brain IRI. Our results revealed that electroacupuncture attenuated IRI in MCAO mice by minishing brain damage and hindering neuronal pyroptosis. Strikingly, it was discovered that electroacupuncture provoked the decrease of succinylation level and enhanced expression of SIRT5. Then, we demonstrated that knockdown of SIRT5 reversed the role of electroacupuncture in cerebral infarct injury and pyroptosis. In terms of mechanism, SIRT5 impeded the succinylation modification of NEK7 at K81 site to downregulate its expression level. Eventually, overexpression of NEK7 abrogated the impacts of electroacupuncture on MCAO mice. In conclusion, electroacupuncture restrained neuronal pyroptosis after cerebral ischemia via desuccinylating NEK7 in a SIRT5-dependent way. |
| format | Article |
| id | doaj-art-a6a3ad8680f34b05a59889499ec87635 |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-a6a3ad8680f34b05a59889499ec876352025-01-10T04:37:03ZengElsevierBrain Research Bulletin1873-27472025-01-01220111173Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylationLili Ma0Meiling Zhang1Ting Chen2Limin Wang3Qilong Deng4Wenzhou Medical University, Chashan University Town, Ouhai District, Wenzhou, Zhejiang 325035, China; Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, ChinaTaizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, China; Luqiao Hospital, Taizhou Enze Medical Center (Group), No 1, West Xialiqiao Road, Luqiao District, Taizhou, Zhejiang 318050, ChinaTaizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, ChinaTaizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, China; Luqiao Hospital, Taizhou Enze Medical Center (Group), No 1, West Xialiqiao Road, Luqiao District, Taizhou, Zhejiang 318050, China; Corresponding authors at: Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, China.Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, China; Luqiao Hospital, Taizhou Enze Medical Center (Group), No 1, West Xialiqiao Road, Luqiao District, Taizhou, Zhejiang 318050, China; Corresponding authors at: Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University, No 150, Ximen Street, Linhai, Taizhou, Zhejiang 317000, China.Ischemic stroke is a leading cause of global death. The treatment of this disease can inevitably result in reperfusion, thereby triggering cerebral ischemia-reperfusion injury (IRI) and neuronal pyroptosis. Electroacupuncture derived from traditional acupuncture has been proven to have favorable effects on ameliorating brain IRI and pyroptosis. Hence, the goal of the current research was to elucidate the mechanism governing electroacupuncture in cerebral IRI. We employed middle cerebral artery occlusion (MCAO) model to induce brain IRI. Our results revealed that electroacupuncture attenuated IRI in MCAO mice by minishing brain damage and hindering neuronal pyroptosis. Strikingly, it was discovered that electroacupuncture provoked the decrease of succinylation level and enhanced expression of SIRT5. Then, we demonstrated that knockdown of SIRT5 reversed the role of electroacupuncture in cerebral infarct injury and pyroptosis. In terms of mechanism, SIRT5 impeded the succinylation modification of NEK7 at K81 site to downregulate its expression level. Eventually, overexpression of NEK7 abrogated the impacts of electroacupuncture on MCAO mice. In conclusion, electroacupuncture restrained neuronal pyroptosis after cerebral ischemia via desuccinylating NEK7 in a SIRT5-dependent way.http://www.sciencedirect.com/science/article/pii/S0361923024003071ElectroacupunctureCerebral ischemiaPyroptosisSIRT5/NEK7Succinylation |
| spellingShingle | Lili Ma Meiling Zhang Ting Chen Limin Wang Qilong Deng Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation Brain Research Bulletin Electroacupuncture Cerebral ischemia Pyroptosis SIRT5/NEK7 Succinylation |
| title | Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation |
| title_full | Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation |
| title_fullStr | Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation |
| title_full_unstemmed | Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation |
| title_short | Electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating SIRT5-mediated NEK7 succinylation |
| title_sort | electroacupuncture inhibits neuronal pyroptosis in ischemic brain injury through modulating sirt5 mediated nek7 succinylation |
| topic | Electroacupuncture Cerebral ischemia Pyroptosis SIRT5/NEK7 Succinylation |
| url | http://www.sciencedirect.com/science/article/pii/S0361923024003071 |
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