Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor
Surface coatings with polyethylene glycol (PEG) polymers have often been employed to improve nanoparticles (NPs) biocompatibility and extend circulation time by reducing protein adsorption. PEGylated NPs benefit from steric hindrance and repulsion effects, which are influenced by PEG molecular weigh...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Nanotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnano.2024.1505757/full |
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| author | Eva Clemente Ruth Mateu Avelino Ferreira Tanja Ludtke Tanja Ludtke Hender Lopez Sergio E. Moya Luigi Lay Mahmoud G. Soliman Marco P. Monopoli |
| author_facet | Eva Clemente Ruth Mateu Avelino Ferreira Tanja Ludtke Tanja Ludtke Hender Lopez Sergio E. Moya Luigi Lay Mahmoud G. Soliman Marco P. Monopoli |
| author_sort | Eva Clemente |
| collection | DOAJ |
| description | Surface coatings with polyethylene glycol (PEG) polymers have often been employed to improve nanoparticles (NPs) biocompatibility and extend circulation time by reducing protein adsorption. PEGylated NPs benefit from steric hindrance and repulsion effects, which are influenced by PEG molecular weight, density, and chain conformation. However, repetitive exposure to PEG can trigger acute and chronic immunological responses as a result of the development of Immunoglobulin G anti-PEG antibodies. NPs functionalisation with glycans has become an emerging approach to increase their biocompatibility as these biomolecules are highly hydrophilic, biocompatible interact with biological receptors expressed in the body, and can be conjugated, controlling their orientation. In this study, we developed a series of gold NPs (AuNPs) coated with PEG linkers of different lengths and conjugated with mannose (Man) or sialic acid (Sia) glycans, and we carried out a detailed characterisation prior to and after exposure to biological fluids to study their behaviour and protein corona formation. Our findings show that the glycan-coated NPs exhibit stabilisation after protein interaction, with Man coatings showing the lowest protein affinity and that the glycans are biologically active and capable of binding to glycan receptors (such as Concanavalin A) despite the presence of a complex protein environment. Results indicate that glycan modification of PEGylated NPs reduces nonspecific interactions while preserving active targeting properties, underscoring their potential for therapeutic applications. |
| format | Article |
| id | doaj-art-a65515e5487a4794a21354311954aca2 |
| institution | Kabale University |
| issn | 2673-3013 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nanotechnology |
| spelling | doaj-art-a65515e5487a4794a21354311954aca22025-01-13T04:11:24ZengFrontiers Media S.A.Frontiers in Nanotechnology2673-30132025-01-01610.3389/fnano.2024.15057571505757Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptorEva Clemente0Ruth Mateu1Avelino Ferreira2Tanja Ludtke3Tanja Ludtke4Hender Lopez5Sergio E. Moya6Luigi Lay7Mahmoud G. Soliman8Marco P. Monopoli9Department of Chemistry, RCSI (Royal College of Surgeons in Ireland), Dublin, IrelandDepartment of Chemistry, University of Milan, Milan, ItalyDepartment of Chemistry, RCSI (Royal College of Surgeons in Ireland), Dublin, IrelandCenter for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Donostia-San Sebastian, SpainDpto Química Orgánica II/ Facultad de Ciencia y Tecnología, Universidad del País Vasco/Euskal Herriko Unibertsitatea, Leioa, Bizkaia, SpainSchool of Physics, Clinical and Optometric Sciences, Technological University Dublin, Dublin, IrelandCenter for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), Donostia-San Sebastian, SpainDepartment of Chemistry, University of Milan, Milan, ItalyDepartment of Chemistry, RCSI (Royal College of Surgeons in Ireland), Dublin, IrelandDepartment of Chemistry, RCSI (Royal College of Surgeons in Ireland), Dublin, IrelandSurface coatings with polyethylene glycol (PEG) polymers have often been employed to improve nanoparticles (NPs) biocompatibility and extend circulation time by reducing protein adsorption. PEGylated NPs benefit from steric hindrance and repulsion effects, which are influenced by PEG molecular weight, density, and chain conformation. However, repetitive exposure to PEG can trigger acute and chronic immunological responses as a result of the development of Immunoglobulin G anti-PEG antibodies. NPs functionalisation with glycans has become an emerging approach to increase their biocompatibility as these biomolecules are highly hydrophilic, biocompatible interact with biological receptors expressed in the body, and can be conjugated, controlling their orientation. In this study, we developed a series of gold NPs (AuNPs) coated with PEG linkers of different lengths and conjugated with mannose (Man) or sialic acid (Sia) glycans, and we carried out a detailed characterisation prior to and after exposure to biological fluids to study their behaviour and protein corona formation. Our findings show that the glycan-coated NPs exhibit stabilisation after protein interaction, with Man coatings showing the lowest protein affinity and that the glycans are biologically active and capable of binding to glycan receptors (such as Concanavalin A) despite the presence of a complex protein environment. Results indicate that glycan modification of PEGylated NPs reduces nonspecific interactions while preserving active targeting properties, underscoring their potential for therapeutic applications.https://www.frontiersin.org/articles/10.3389/fnano.2024.1505757/fullgold nanoparticlesglycansprotein coronacolloidal stabilityactive targetingPEGylation processes |
| spellingShingle | Eva Clemente Ruth Mateu Avelino Ferreira Tanja Ludtke Tanja Ludtke Hender Lopez Sergio E. Moya Luigi Lay Mahmoud G. Soliman Marco P. Monopoli Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor Frontiers in Nanotechnology gold nanoparticles glycans protein corona colloidal stability active targeting PEGylation processes |
| title | Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| title_full | Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| title_fullStr | Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| title_full_unstemmed | Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| title_short | Monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| title_sort | monosaccharide coatings on nanoparticles affect protein corona formation but not the interaction with their binding receptor |
| topic | gold nanoparticles glycans protein corona colloidal stability active targeting PEGylation processes |
| url | https://www.frontiersin.org/articles/10.3389/fnano.2024.1505757/full |
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