Generation of an inducible dCas9-SAM human PSC line for endogenous gene activation

Generation of an inducible dCas9-SAM human PSC line for endogenous gene activation

The CRISPR/Cas9 system has transformed genome editing by enabling precise modifications for diverse applications. Recent advancements, including base editing and prime editing, have expanded its utility beyond conventional gene knock-out and knock-in strategies. Additionally, several catalytically d...

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Bibliographic Details
Main Authors: Paolo Petazzi, Francisco Gutierrez-Agüera, Heleia Roca-Ho, Julio Castaño, Clara Bueno, Niuska Alvarez, Lesley M. Forrester, Ana Sevilla, Antonella Fidanza, Pablo Menendez
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
human PSCs
ISAM
dead-Cas9 activation
gene expression
AAVS1 safe harbour locus
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2024.1484955/full
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https://www.frontiersin.org/articles/10.3389/fcell.2024.1484955/full

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