Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities
The aim of this study was to quantitatively analyse lactobionic acid obtained from bioreactor cultures using whey as a liquid medium with bacteria of the <i>Pseudomonas taetrolens</i> species. The most important culture parameters affecting the production of the acid are indicated and ev...
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/22/5400 |
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| author | Kamila Goderska Wojciech Juzwa Tomasz M. Karpiński |
| author_facet | Kamila Goderska Wojciech Juzwa Tomasz M. Karpiński |
| author_sort | Kamila Goderska |
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| description | The aim of this study was to quantitatively analyse lactobionic acid obtained from bioreactor cultures using whey as a liquid medium with bacteria of the <i>Pseudomonas taetrolens</i> species. The most important culture parameters affecting the production of the acid are indicated and evaluated. The highest lactobionic acid yield was 37.42 g/L, selecting the appropriate strain (<i>Pseudomonas taetrolens</i> 4′) and environmental conditions (2% lactose concentration in the bioreactor). The amount of lactose and lactobionic acid was determined by high-performance liquid chromatography. Microorganism analysis was also carried out using a flow cytometer with imaging to study the metabolic activity of microorganisms during lactobionic acid production. In addition, during the study, Bifidobacteria were microencapsulated with lactobionic acid and their survival was evaluated in an in vitro model of the gastrointestinal tract, checking the prebiotic properties of the acid. The highest number of viable cells in the microcapsules after digestion was obtained using the <i>Bifidobacterium bifidum</i> strain DSM 20082. The antagonistic activity of lactobionic acid was also analysed. |
| format | Article |
| id | doaj-art-a4bcd4525c5c4b268ea4da423c38bc0a |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-a4bcd4525c5c4b268ea4da423c38bc0a2024-11-26T18:15:50ZengMDPI AGMolecules1420-30492024-11-012922540010.3390/molecules29225400Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological ActivitiesKamila Goderska0Wojciech Juzwa1Tomasz M. Karpiński2Department of Food Technology of Plant Origin, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 31, 60-624 Poznan, PolandDepartment of Biotechnology and Food Microbiology, Faculty of Food Science and Nutrition, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznan, PolandChair and Department of Medical Microbiology, Poznań University of Medical Sciences, Rokietnicka 10, 60-806 Poznan, PolandThe aim of this study was to quantitatively analyse lactobionic acid obtained from bioreactor cultures using whey as a liquid medium with bacteria of the <i>Pseudomonas taetrolens</i> species. The most important culture parameters affecting the production of the acid are indicated and evaluated. The highest lactobionic acid yield was 37.42 g/L, selecting the appropriate strain (<i>Pseudomonas taetrolens</i> 4′) and environmental conditions (2% lactose concentration in the bioreactor). The amount of lactose and lactobionic acid was determined by high-performance liquid chromatography. Microorganism analysis was also carried out using a flow cytometer with imaging to study the metabolic activity of microorganisms during lactobionic acid production. In addition, during the study, Bifidobacteria were microencapsulated with lactobionic acid and their survival was evaluated in an in vitro model of the gastrointestinal tract, checking the prebiotic properties of the acid. The highest number of viable cells in the microcapsules after digestion was obtained using the <i>Bifidobacterium bifidum</i> strain DSM 20082. The antagonistic activity of lactobionic acid was also analysed.https://www.mdpi.com/1420-3049/29/22/5400lactobionic acid<i>Pseudomonas taetrolens</i>wheylactoseflow cytometry<i>Bifidobacterium</i> |
| spellingShingle | Kamila Goderska Wojciech Juzwa Tomasz M. Karpiński Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities Molecules lactobionic acid <i>Pseudomonas taetrolens</i> whey lactose flow cytometry <i>Bifidobacterium</i> |
| title | Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities |
| title_full | Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities |
| title_fullStr | Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities |
| title_full_unstemmed | Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities |
| title_short | Quantitative Analysis of Lactobionic Acid in Bioreactor Cultures and Selected Biological Activities |
| title_sort | quantitative analysis of lactobionic acid in bioreactor cultures and selected biological activities |
| topic | lactobionic acid <i>Pseudomonas taetrolens</i> whey lactose flow cytometry <i>Bifidobacterium</i> |
| url | https://www.mdpi.com/1420-3049/29/22/5400 |
| work_keys_str_mv | AT kamilagoderska quantitativeanalysisoflactobionicacidinbioreactorculturesandselectedbiologicalactivities AT wojciechjuzwa quantitativeanalysisoflactobionicacidinbioreactorculturesandselectedbiologicalactivities AT tomaszmkarpinski quantitativeanalysisoflactobionicacidinbioreactorculturesandselectedbiologicalactivities |