Assisted Isolation of Camelliagenin B from <i>Camellia oliefera</i> Seed Cake Meal and Microbial Transformation by <i>Bacillus subtilis</i> ATCC 6633, <i>Bacillus megaterium</i> CGMCC 1.1741, and <i>Streptomyces gresius</i> ATCC 13273

Assisted Isolation of Camelliagenin B from <i>Camellia oliefera</i> Seed Cake Meal and Microbial Transformation by <i>Bacillus subtilis</i> ATCC 6633, <i>Bacillus megaterium</i> CGMCC 1.1741, and <i>Streptomyces gresius</i> ATCC 13273

This study investigates the potential for the microbial transformation of camelliagenin B, a saponin derived from <i>Camellia oleifera</i> seed cake meal, to develop novel metabolites. We employed three microbial strains, specifically <i>Bacillus subtilis</i> ATCC 6633, <i...

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Bibliographic Details
Main Authors: Richa Raj, Jingling Zhang, Yanyan Meng, Xuewa Jiang, Wei Wang, Jian Zhang, Boyang Yu
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Fermentation
Subjects:
camelliagenin B
tea sapogenin
microbial transformation
by-products processing
<i>Camellia oliefera</i> seed pomace
Online Access:https://www.mdpi.com/2311-5637/11/7/407
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Summary:This study investigates the potential for the microbial transformation of camelliagenin B, a saponin derived from <i>Camellia oleifera</i> seed cake meal, to develop novel metabolites. We employed three microbial strains, specifically <i>Bacillus subtilis</i> ATCC 6633, <i>Bacillus megaterium</i> CGMCC 1.1741, and <i>Streptomyces griseus</i> ATCC 13273, to biotransform camelliagenin B into its derivatives. The compounds were purified and separated using chromatographic techniques, such as high-performance liquid chromatography (HPLC). Structural identification was carried out using spectroscopic methods, including nuclear magnetic resonance (NMR) and mass spectrometry (MS). Ten bioactive compounds were obtained (<b>1a</b>-<b>1j</b>), of which nine were novel with multiple tailoring reactions, such as allyl oxidation, C-C double-bond rearrangement, hydroxylation, dehydrogenation, and glycosylation, observed in camelliagenin B analogs. The structures of these compounds were determined by 1D/2D NMR and HR-ESI-MS analysis. Therefore, this study showcases the capacity of microbial transformation as a sustainable and environmentally friendly method for generating bioactive compounds from <i>C. oleifera</i> seed cake meals. The individual chemicals can potentially facilitate the design of novel medicinal agents, functional foods, and natural preservatives.
ISSN:2311-5637

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