Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta
Abstract The emergence of multidrug-resistant pathogens underscores the urgent need for novel antimicrobial agents. In this study, ten endophytic fungal isolates (Ls1–Ls10) were isolated for the first time from Lavandula stricta and evaluated for their antimicrobial activity against Staphylococcus a...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-07627-w |
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| author | Samy Selim Mohamed H. Moustafa Mohammed S. Almuhayawi Hattan S. Gattan Mohammed H. Alruhaili Mohanned Talal Alharbi Soad K. Al Jaouni Hanan M. Alharbi Fayza Kouadri Amer M. Abdelaziz |
| author_facet | Samy Selim Mohamed H. Moustafa Mohammed S. Almuhayawi Hattan S. Gattan Mohammed H. Alruhaili Mohanned Talal Alharbi Soad K. Al Jaouni Hanan M. Alharbi Fayza Kouadri Amer M. Abdelaziz |
| author_sort | Samy Selim |
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| description | Abstract The emergence of multidrug-resistant pathogens underscores the urgent need for novel antimicrobial agents. In this study, ten endophytic fungal isolates (Ls1–Ls10) were isolated for the first time from Lavandula stricta and evaluated for their antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella oxytoca, and Candida albicans. The most potent fungal isolate Ls1 was identified as Sarocladium kiliense using morphological and molecular techniques. Phytochemical analysis indicated that the S. kiliense extract is abundant in bioactive compounds, including phenolics, tannins, flavonoids, and alkaloids. The GC mass analysis proved the presence of 41 active compounds in the S. kiliense. Extract including; Benzene, (1-propylnonyl) (9.87%), Hexadecanoic acid (8.05%), Prostaglandin A1-biotin (6.77%), Docosene (6.69%), Octadecenoic acid (5.55%), and 1-Nonadecene (5.16%). The crude extract of S. kiliense showed outstanding anticancer activity against cancerous Hep-G2 and MCF-7 cell lines with IC50 of 31.7 and 49.8 µg/ml, respectively. This isolate exhibited significant antimicrobial activity, with inhibition zones ranging from 16.1 ± 0.1 mm to 35.5 mm. MICs varied between 62.5 and 250 µg/mL. S. kiliense exhibited antioxidant activity and antibiofilm activities. The S. kiliense extract demonstrated concentration-dependent antibiofilm activity. In conclusion, S. kiliense as a hopeful home of bioactive combinations with potent antimicrobial, antioxidant, anticancer, and antibiofilm activities, offering the potential for combating multidrug-resistant pathogens and therapeutic applications. |
| format | Article |
| id | doaj-art-a40e96b19f0c45e2be039479491a0cf9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-a40e96b19f0c45e2be039479491a0cf92025-08-20T03:45:26ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-07627-wPhytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula strictaSamy Selim0Mohamed H. Moustafa1Mohammed S. Almuhayawi2Hattan S. Gattan3Mohammed H. Alruhaili4Mohanned Talal Alharbi5Soad K. Al Jaouni6Hanan M. Alharbi7Fayza Kouadri8Amer M. Abdelaziz9Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf UniversityCollege of Pharmacy, Al-Farahidi UniversityDepartment of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz UniversityDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz UniversityDepartment of Clinical Microbiology and Immunology, Faculty of Medicine, King Abdulaziz UniversityDepartment of Basic Medical Sciences, College of Medicine, University of JeddahDepartment of Hematology/Oncology, Scientific Chair of Prophetic Medicine Application, Faculty of Medicine, King Abdulaziz UniversityDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman UniversityDepartment of Medical and Clinical laboratory Technology, Faculty of Applied Medical Sciences, Applied Science Private UniversityBotany and Microbiology Department, Faculty of Science, Al-Azhar UniversityAbstract The emergence of multidrug-resistant pathogens underscores the urgent need for novel antimicrobial agents. In this study, ten endophytic fungal isolates (Ls1–Ls10) were isolated for the first time from Lavandula stricta and evaluated for their antimicrobial activity against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella oxytoca, and Candida albicans. The most potent fungal isolate Ls1 was identified as Sarocladium kiliense using morphological and molecular techniques. Phytochemical analysis indicated that the S. kiliense extract is abundant in bioactive compounds, including phenolics, tannins, flavonoids, and alkaloids. The GC mass analysis proved the presence of 41 active compounds in the S. kiliense. Extract including; Benzene, (1-propylnonyl) (9.87%), Hexadecanoic acid (8.05%), Prostaglandin A1-biotin (6.77%), Docosene (6.69%), Octadecenoic acid (5.55%), and 1-Nonadecene (5.16%). The crude extract of S. kiliense showed outstanding anticancer activity against cancerous Hep-G2 and MCF-7 cell lines with IC50 of 31.7 and 49.8 µg/ml, respectively. This isolate exhibited significant antimicrobial activity, with inhibition zones ranging from 16.1 ± 0.1 mm to 35.5 mm. MICs varied between 62.5 and 250 µg/mL. S. kiliense exhibited antioxidant activity and antibiofilm activities. The S. kiliense extract demonstrated concentration-dependent antibiofilm activity. In conclusion, S. kiliense as a hopeful home of bioactive combinations with potent antimicrobial, antioxidant, anticancer, and antibiofilm activities, offering the potential for combating multidrug-resistant pathogens and therapeutic applications.https://doi.org/10.1038/s41598-025-07627-wSarocladiumAntibiofilmAntioxidantAntimicrobialAnticancerDiseases |
| spellingShingle | Samy Selim Mohamed H. Moustafa Mohammed S. Almuhayawi Hattan S. Gattan Mohammed H. Alruhaili Mohanned Talal Alharbi Soad K. Al Jaouni Hanan M. Alharbi Fayza Kouadri Amer M. Abdelaziz Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta Scientific Reports Sarocladium Antibiofilm Antioxidant Antimicrobial Anticancer Diseases |
| title | Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta |
| title_full | Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta |
| title_fullStr | Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta |
| title_full_unstemmed | Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta |
| title_short | Phytochemical profiling and evaluation of antioxidant, anticancer, antimicrobial and antibiofilm activities of endophytic fungi isolated from Lavandula stricta |
| title_sort | phytochemical profiling and evaluation of antioxidant anticancer antimicrobial and antibiofilm activities of endophytic fungi isolated from lavandula stricta |
| topic | Sarocladium Antibiofilm Antioxidant Antimicrobial Anticancer Diseases |
| url | https://doi.org/10.1038/s41598-025-07627-w |
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