Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis
Abstract Myeloperoxidase (MPO) plays an important role in the immune response of human neutrophils and has been implicated in autoimmune conditions, cardiovascular disorders, and neurodegeneration. Current methods to detect MPO activity rely on the detection of HOCl using activatable probes or requi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-024-01338-5 |
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| author | Enebie Ramos Cáceres Lotte Kemperman Kimberly M. Bonger |
| author_facet | Enebie Ramos Cáceres Lotte Kemperman Kimberly M. Bonger |
| author_sort | Enebie Ramos Cáceres |
| collection | DOAJ |
| description | Abstract Myeloperoxidase (MPO) plays an important role in the immune response of human neutrophils and has been implicated in autoimmune conditions, cardiovascular disorders, and neurodegeneration. Current methods to detect MPO activity rely on the detection of HOCl using activatable probes or require challenging experimental procedures. Therefore, these tools provide limited information about the dynamics and localization of MPO in complex molecular processes such as NETosis in real time. In this study, we report a ‘’turn-on” activity-based probe that fluoresces exclusively upon binding to MPO, exhibits minimal background fluorescence in buffered aqueous media, and is blocked by MPO inhibitors. Our probe facilitates real-time imaging of direct MPO activity in human neutrophils and HL-60-derived granulocytes during NETosis under wash-free conditions. Furthermore, it allows for the discrimination between different triggers of NETosis in human neutrophils. These findings hold promise for advancing our understanding of the role of MPO in immune responses and inflammatory conditions. |
| format | Article |
| id | doaj-art-a30fd154e89b4392aaee4d8a9a03b54e |
| institution | Kabale University |
| issn | 2399-3669 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj-art-a30fd154e89b4392aaee4d8a9a03b54e2024-11-17T12:14:15ZengNature PortfolioCommunications Chemistry2399-36692024-11-01711710.1038/s42004-024-01338-5Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosisEnebie Ramos Cáceres0Lotte Kemperman1Kimberly M. Bonger2Department of Synthetic Organic Chemistry, Institute for Molecules and Materials, Radboud UniversityDepartment of Synthetic Organic Chemistry, Institute for Molecules and Materials, Radboud UniversityDepartment of Synthetic Organic Chemistry, Institute for Molecules and Materials, Radboud UniversityAbstract Myeloperoxidase (MPO) plays an important role in the immune response of human neutrophils and has been implicated in autoimmune conditions, cardiovascular disorders, and neurodegeneration. Current methods to detect MPO activity rely on the detection of HOCl using activatable probes or require challenging experimental procedures. Therefore, these tools provide limited information about the dynamics and localization of MPO in complex molecular processes such as NETosis in real time. In this study, we report a ‘’turn-on” activity-based probe that fluoresces exclusively upon binding to MPO, exhibits minimal background fluorescence in buffered aqueous media, and is blocked by MPO inhibitors. Our probe facilitates real-time imaging of direct MPO activity in human neutrophils and HL-60-derived granulocytes during NETosis under wash-free conditions. Furthermore, it allows for the discrimination between different triggers of NETosis in human neutrophils. These findings hold promise for advancing our understanding of the role of MPO in immune responses and inflammatory conditions.https://doi.org/10.1038/s42004-024-01338-5 |
| spellingShingle | Enebie Ramos Cáceres Lotte Kemperman Kimberly M. Bonger Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis Communications Chemistry |
| title | Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis |
| title_full | Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis |
| title_fullStr | Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis |
| title_full_unstemmed | Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis |
| title_short | Environment-sensitive turn-on fluorescent probe enables live cell imaging of myeloperoxidase activity during NETosis |
| title_sort | environment sensitive turn on fluorescent probe enables live cell imaging of myeloperoxidase activity during netosis |
| url | https://doi.org/10.1038/s42004-024-01338-5 |
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