Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats
Abstract The current study aimed to examine how smooth muscle contractile properties and expression of functional proteins in the urethra and prostate are affected in an animal model of chronic prostatitis/chronic pelvic pain syndrome (CPPS). Thirty-two male Sprague-Dawley rats received an intrapros...
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Nature Portfolio
2025-06-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-06531-7 |
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| author | Ozgu Aydogdu Gwenaelle Black Patrik Aronsson Thomas Carlsson Michael Winder |
| author_facet | Ozgu Aydogdu Gwenaelle Black Patrik Aronsson Thomas Carlsson Michael Winder |
| author_sort | Ozgu Aydogdu |
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| description | Abstract The current study aimed to examine how smooth muscle contractile properties and expression of functional proteins in the urethra and prostate are affected in an animal model of chronic prostatitis/chronic pelvic pain syndrome (CPPS). Thirty-two male Sprague-Dawley rats received an intraprostatic injection with saline or zymosan, serving as control and a model for CPPS, respectively. Two weeks later, the urethra and dorsal prostate were excised and studied functionally in organ baths. Following this, protein expression and urethral inflammation was examined immunohistochemically. Neither prostate nor urethra showed any significant changes in contractility compared to the control group, despite a tendency for increased cholinergic contractile responses in the CPPS urethra. Induction of CPPS led to an increased expression of muscarinic M3 receptors in the urethra. In the prostate, there were no significant differences in protein expression. HE staining showed no signs of inflammation in the urethra in either group. Previous studies have shown that CPPS can alter bladder contractility. Currently, CPPS did not affect contractile responses in neither prostate nor urethra. These findings are consistent with the theory of prostate-to-bladder cross-organ sensitization. Future studies exploring this may be of great relevance in the development of new treatment options for CPPS. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a1f40666a997471896b51d464284f6b82025-08-20T03:47:14ZengNature PortfolioScientific Reports2045-23222025-06-0115111110.1038/s41598-025-06531-7Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in ratsOzgu Aydogdu0Gwenaelle Black1Patrik Aronsson2Thomas Carlsson3Michael Winder4Department of Urology, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Pharmacology, The Sahlgrenska Academy, University of GothenburgDepartment of Pharmacology, The Sahlgrenska Academy, University of GothenburgDepartment of Pharmacology, The Sahlgrenska Academy, University of GothenburgDepartment of Pharmacology, The Sahlgrenska Academy, University of GothenburgAbstract The current study aimed to examine how smooth muscle contractile properties and expression of functional proteins in the urethra and prostate are affected in an animal model of chronic prostatitis/chronic pelvic pain syndrome (CPPS). Thirty-two male Sprague-Dawley rats received an intraprostatic injection with saline or zymosan, serving as control and a model for CPPS, respectively. Two weeks later, the urethra and dorsal prostate were excised and studied functionally in organ baths. Following this, protein expression and urethral inflammation was examined immunohistochemically. Neither prostate nor urethra showed any significant changes in contractility compared to the control group, despite a tendency for increased cholinergic contractile responses in the CPPS urethra. Induction of CPPS led to an increased expression of muscarinic M3 receptors in the urethra. In the prostate, there were no significant differences in protein expression. HE staining showed no signs of inflammation in the urethra in either group. Previous studies have shown that CPPS can alter bladder contractility. Currently, CPPS did not affect contractile responses in neither prostate nor urethra. These findings are consistent with the theory of prostate-to-bladder cross-organ sensitization. Future studies exploring this may be of great relevance in the development of new treatment options for CPPS.https://doi.org/10.1038/s41598-025-06531-7Chronic pelvic pain syndromeProstatitisUrethraProstateOrgan bathIn vitro |
| spellingShingle | Ozgu Aydogdu Gwenaelle Black Patrik Aronsson Thomas Carlsson Michael Winder Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats Scientific Reports Chronic pelvic pain syndrome Prostatitis Urethra Prostate Organ bath In vitro |
| title | Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| title_full | Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| title_fullStr | Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| title_full_unstemmed | Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| title_short | Induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| title_sort | induction of chronic prostatitis does not alter the innate contractile properties of the prostate or urethra in rats |
| topic | Chronic pelvic pain syndrome Prostatitis Urethra Prostate Organ bath In vitro |
| url | https://doi.org/10.1038/s41598-025-06531-7 |
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