ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon
Citric acid is an important organic acid with wide applications and diverse biological functionality. As the predominant organic acid in lemons, citric acid plays a crucial role in determining the flavor of citrus, especially in lemons. ATP citrate lyase (ACL, EC4.1.3.8) is the keg gene in citric ac...
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2024-12-01
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| author | Chuang Lu Wenhui Yang Huaxi Zhang Yanrong Wu Huina Meng Lifeng Lv Wanping Lu Dongmei Zhao Guixiang Huang |
| author_facet | Chuang Lu Wenhui Yang Huaxi Zhang Yanrong Wu Huina Meng Lifeng Lv Wanping Lu Dongmei Zhao Guixiang Huang |
| author_sort | Chuang Lu |
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| description | Citric acid is an important organic acid with wide applications and diverse biological functionality. As the predominant organic acid in lemons, citric acid plays a crucial role in determining the flavor of citrus, especially in lemons. ATP citrate lyase (ACL, EC4.1.3.8) is the keg gene in citric acid metabolism. Several research studies on ACL only focused on high-sugar- and low-acid-content citrus varieties; however, the ACL mechanism in lemons with high acid and low sugar levels remains undetermined. In this study, a key candidate gene, <i>ClACLB-1</i>, for citrate cleavage was identified from the genome data of ‘Xiangshui’ lemon [<i>Citrus limon</i> (L.) Burm f.]. The putative protein coded by the gene <i>ClACLB</i>-1 is localized in the nuclear and cell membranes. The <i>ClACLB</i>-1 gene was expressed in all tissues, with the highest expression in male flowers and the lowest expression in mature fruits; the expression decreased during lemon fruit development. The overexpression of <i>ClACLB</i>-1 in transgenic tomatoes significantly increases the activity of citrate lyase, which subsequently reduces citric acid content. This study clarified the function of the <i>ClACLB-1</i> gene in cleaving citric acid, provided new insights into the citric acid metabolism of citrus, and offered a theoretical reference for reducing acid and increasing sugar in citrus to improve fruit quality. It also helped to enhance the understanding of the metabolism and role of citrate in plants. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| series | Plants |
| spelling | doaj-art-a1bbf46a2e384f28be2d95f0d58b98262025-01-10T13:19:37ZengMDPI AGPlants2223-77472024-12-011415310.3390/plants14010053ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in LemonChuang Lu0Wenhui Yang1Huaxi Zhang2Yanrong Wu3Huina Meng4Lifeng Lv5Wanping Lu6Dongmei Zhao7Guixiang Huang8College of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaCitric acid is an important organic acid with wide applications and diverse biological functionality. As the predominant organic acid in lemons, citric acid plays a crucial role in determining the flavor of citrus, especially in lemons. ATP citrate lyase (ACL, EC4.1.3.8) is the keg gene in citric acid metabolism. Several research studies on ACL only focused on high-sugar- and low-acid-content citrus varieties; however, the ACL mechanism in lemons with high acid and low sugar levels remains undetermined. In this study, a key candidate gene, <i>ClACLB-1</i>, for citrate cleavage was identified from the genome data of ‘Xiangshui’ lemon [<i>Citrus limon</i> (L.) Burm f.]. The putative protein coded by the gene <i>ClACLB</i>-1 is localized in the nuclear and cell membranes. The <i>ClACLB</i>-1 gene was expressed in all tissues, with the highest expression in male flowers and the lowest expression in mature fruits; the expression decreased during lemon fruit development. The overexpression of <i>ClACLB</i>-1 in transgenic tomatoes significantly increases the activity of citrate lyase, which subsequently reduces citric acid content. This study clarified the function of the <i>ClACLB-1</i> gene in cleaving citric acid, provided new insights into the citric acid metabolism of citrus, and offered a theoretical reference for reducing acid and increasing sugar in citrus to improve fruit quality. It also helped to enhance the understanding of the metabolism and role of citrate in plants.https://www.mdpi.com/2223-7747/14/1/53lemoncitric acidgenome-wide identification<i>ClACLB-1</i>gene function |
| spellingShingle | Chuang Lu Wenhui Yang Huaxi Zhang Yanrong Wu Huina Meng Lifeng Lv Wanping Lu Dongmei Zhao Guixiang Huang ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon Plants lemon citric acid genome-wide identification <i>ClACLB-1</i> gene function |
| title | ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon |
| title_full | ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon |
| title_fullStr | ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon |
| title_full_unstemmed | ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon |
| title_short | ATP Citrate Lyase <i>ClACLB-1</i> Facilitates Citrate Cleavage in Lemon |
| title_sort | atp citrate lyase i claclb 1 i facilitates citrate cleavage in lemon |
| topic | lemon citric acid genome-wide identification <i>ClACLB-1</i> gene function |
| url | https://www.mdpi.com/2223-7747/14/1/53 |
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