The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro
The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2019-12-01
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| Series: | Journal of Parasite Science |
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| Online Access: | https://e-journal.unair.ac.id/JoPS/article/view/16427 |
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| author | Amelia Dwita Safitri Iwan Sahrial Hamid Poedji Hastutiek Setiawan Koesdarto Rahmi Sugihartuti Endang Suprihati |
| author_facet | Amelia Dwita Safitri Iwan Sahrial Hamid Poedji Hastutiek Setiawan Koesdarto Rahmi Sugihartuti Endang Suprihati |
| author_sort | Amelia Dwita Safitri |
| collection | DOAJ |
| description | The aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours. |
| format | Article |
| id | doaj-art-a0c7170a9492466fbc6f75ee8a5fa6b7 |
| institution | Kabale University |
| issn | 2599-0993 2656-5331 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Parasite Science |
| spelling | doaj-art-a0c7170a9492466fbc6f75ee8a5fa6b72025-01-09T04:38:15ZengUniversitas AirlanggaJournal of Parasite Science2599-09932656-53312019-12-0131192210.20473/jops.v3i1.1642713384The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in VitroAmelia Dwita Safitri0Iwan Sahrial Hamid1Poedji Hastutiek2Setiawan Koesdarto3Rahmi Sugihartuti4Endang Suprihati5Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Basic Veterinary Medicine - Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Veterinary Parasitology - Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Veterinary Parasitology - Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Basic Veterinary Medicine - Faculty of Veterinary Medicine, Universitas AirlanggaDepartment of Veterinary Parasitology - Faculty of Veterinary Medicine, Universitas AirlanggaThe aims of this study is to know the anthelmintic activity of ethanol extract of bitter leaf (Vernonia amygdalina) against Ascaridia galli worm in Vitro, as well as knowing effective concentration 50 (EC50) and lethal time 50 (LT50). Method that used in the research was completely randomized design. There were five treatments of physiological NaCl solution (K-), piperazine sitrate (K+), etanol extract of bitter leaf 0,35% (P1), etanol extract of bitter leaf 1,4% (P2), etanol extract of bitter leaf 4,2% (P3), and each treatment was done in four replications. This research used ten Ascaridia galli in each treatment for all replications. The observation and recording of dead Ascaridia galli was done at 2, 4, 6, 8, 10 and 12 hours. Ascaridia galli were declared dead if there was no movement when disturbed by anatomy tweezer and when dipped in slightly warm water (50°C). The obtained data was analyzed using ANAVA and continued with Duncan Multiple Range Test. The result of this research show that etanol extract of bitter leaf (Vernonia amygdalina) has anthelmintic effects against Ascaridia galli worm in Vitro. In the extract with 4,2% concentration, there is anthelmintic property that almost the same as Piperazine sitrate 10 mg/ml. the higher the concentration of extract, the higher the property of anthelmintic. In probit analysis show that EC50 achieved by concentration 2.093% with the low concentration of .002% and the highest concentration of 3.632%. LT50 of ethanol extract of bitter leaf was 0.35% at 10.323 hours, 1.4% at 9.800 hours, 4.2% at 7.864 hours and Piperazine sitrate 10 mg/ml at 9.013 hours.https://e-journal.unair.ac.id/JoPS/article/view/16427vernonia amygdalinapiperazine sitrateascaridia gallianthelmintic |
| spellingShingle | Amelia Dwita Safitri Iwan Sahrial Hamid Poedji Hastutiek Setiawan Koesdarto Rahmi Sugihartuti Endang Suprihati The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro Journal of Parasite Science vernonia amygdalina piperazine sitrate ascaridia galli anthelmintic |
| title | The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro |
| title_full | The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro |
| title_fullStr | The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro |
| title_full_unstemmed | The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro |
| title_short | The Anthelmintic Activity of Ethanol Extract of Bitter Leaf (Vernonia amygdalina) Against Ascaridia galli Worm in Vitro |
| title_sort | anthelmintic activity of ethanol extract of bitter leaf vernonia amygdalina against ascaridia galli worm in vitro |
| topic | vernonia amygdalina piperazine sitrate ascaridia galli anthelmintic |
| url | https://e-journal.unair.ac.id/JoPS/article/view/16427 |
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