Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market
Abstract This study aimed to investigate the temporal accumulation of odor fatty acids (OFAs) in the dorsal subcutaneous adipose tissue, and uncover their dynamic regulatory metabolic pathways from the transcriptomic perspective in lambs from birth to market. Thirty-two Hulun Buir lambs were selecte...
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| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-024-11161-w |
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| author | Yechan Zhao Zhangyan Li Huimin Ou Zhiliang Tan Jinzhen Jiao |
| author_facet | Yechan Zhao Zhangyan Li Huimin Ou Zhiliang Tan Jinzhen Jiao |
| author_sort | Yechan Zhao |
| collection | DOAJ |
| description | Abstract This study aimed to investigate the temporal accumulation of odor fatty acids (OFAs) in the dorsal subcutaneous adipose tissue, and uncover their dynamic regulatory metabolic pathways from the transcriptomic perspective in lambs from birth to market. Thirty-two Hulun Buir lambs were selected and randomly assigned to four different sampling stages following their growth trajectories: neonatal (day 1), weaning (day 75), mid-fattening (day 150), and late-fattening (day 180) stages. Results indicated that the contents of three OFAs increased progressively as lambs matured, with the most drastic change occurred at mid-fattening vs. weaning. The dynamic transcriptomic profiles exhibited two distinct phases, with differentially expressed genes (DEGs) before weaning were involved in immune homeostasis, whereas those after weaning were associated with nutrient metabolism. Furthermore, DEGs involved in lipid metabolism and branch-chain amino acid degradation pathways exhibited surge in expression at mid-fattening vs. weaning, with acetyl-CoA and branched-chain-CoA as intermediates, and driven by regulation of PPAR and AMPK signaling pathways. Overall, our findings provided novel insight into the critical time window and pivotal candidate genes of OFA synthesis in the adipose tissue, which will assist with the targeted development of nutritional strategies to inhibit OFA accumulation of lambs. |
| format | Article |
| id | doaj-art-a0b1bc07b8ca4eefbeb4de218a02a9ba |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-a0b1bc07b8ca4eefbeb4de218a02a9ba2025-01-05T12:09:28ZengBMCBMC Genomics1471-21642024-12-0125111210.1186/s12864-024-11161-wInferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to marketYechan Zhao0Zhangyan Li1Huimin Ou2Zhiliang Tan3Jinzhen Jiao4CAS Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of SciencesLaboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal UniversityCAS Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of SciencesCAS Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of SciencesCAS Key Laboratory of Agroecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of SciencesAbstract This study aimed to investigate the temporal accumulation of odor fatty acids (OFAs) in the dorsal subcutaneous adipose tissue, and uncover their dynamic regulatory metabolic pathways from the transcriptomic perspective in lambs from birth to market. Thirty-two Hulun Buir lambs were selected and randomly assigned to four different sampling stages following their growth trajectories: neonatal (day 1), weaning (day 75), mid-fattening (day 150), and late-fattening (day 180) stages. Results indicated that the contents of three OFAs increased progressively as lambs matured, with the most drastic change occurred at mid-fattening vs. weaning. The dynamic transcriptomic profiles exhibited two distinct phases, with differentially expressed genes (DEGs) before weaning were involved in immune homeostasis, whereas those after weaning were associated with nutrient metabolism. Furthermore, DEGs involved in lipid metabolism and branch-chain amino acid degradation pathways exhibited surge in expression at mid-fattening vs. weaning, with acetyl-CoA and branched-chain-CoA as intermediates, and driven by regulation of PPAR and AMPK signaling pathways. Overall, our findings provided novel insight into the critical time window and pivotal candidate genes of OFA synthesis in the adipose tissue, which will assist with the targeted development of nutritional strategies to inhibit OFA accumulation of lambs.https://doi.org/10.1186/s12864-024-11161-wLamb meatOdor fatty acidsDynamic accumulationAdipose transcriptomeFrom birth to market |
| spellingShingle | Yechan Zhao Zhangyan Li Huimin Ou Zhiliang Tan Jinzhen Jiao Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market BMC Genomics Lamb meat Odor fatty acids Dynamic accumulation Adipose transcriptome From birth to market |
| title | Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market |
| title_full | Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market |
| title_fullStr | Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market |
| title_full_unstemmed | Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market |
| title_short | Inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of Hulun Buir sheep from birth to market |
| title_sort | inferring transcriptomic dynamics implicated in odor fatty acid accumulation in adipose tissue of hulun buir sheep from birth to market |
| topic | Lamb meat Odor fatty acids Dynamic accumulation Adipose transcriptome From birth to market |
| url | https://doi.org/10.1186/s12864-024-11161-w |
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