Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta
Abstract Tuta absoluta is one of the most destructive pests of tomatoes. Chemical insecticides used to control this leafminer harm all organisms, increasing the risk to public health and the environment. Developing natural alternatives, such as bioinsecticides formulated from essential plant oils, i...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85606-x |
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| author | Krache Farial Boualem Malika Benourad Fouzia Touzout Nabil Mihoub Adil Subhan Danish Sulaiman Ali Alharbi Mohammad Javed Ansari Abdullah A. Alarfaj |
| author_facet | Krache Farial Boualem Malika Benourad Fouzia Touzout Nabil Mihoub Adil Subhan Danish Sulaiman Ali Alharbi Mohammad Javed Ansari Abdullah A. Alarfaj |
| author_sort | Krache Farial |
| collection | DOAJ |
| description | Abstract Tuta absoluta is one of the most destructive pests of tomatoes. Chemical insecticides used to control this leafminer harm all organisms, increasing the risk to public health and the environment. Developing natural alternatives, such as bioinsecticides formulated from essential plant oils, is a key strategy to address this problem. These volatile compounds, derived from the secondary metabolic pathways of plants, exhibit targeted activity against specific pest species. Their use is consistent with an environmentally responsible framework that reduces adverse impacts on ecosystems, protects non-target organisms, safeguards human health, and enhances the efficacy of integrated crop management systems. This study aims to determine the chemical composition of the essential oils (EOs) of from round leaf mint (Mentha rotundifolia) and crown chrysanthemum (Chrysanthemum coronarium) and to evaluate their toxicity to T. absoluta larvae in-vitro. The chemical composition of EOs obtained by steam distillation from the leaves of the plants was analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Indeed, 77 volatile compounds representing 98.19% of the total oil of M. rotundifolia, including cyclobutane acetonitrile, 1-methyl-2-(1-methyl ethenyl)-, terpinene-4-ol, p-menthane, germacrene D, caryophyllene and myrcene, were the main compounds. However, farnesene, myrcene, eugenol, germacrene D, phytol, and pinene were significant components among 69 compounds representing 95.39% of the total oil of C. coronarium. Results showed that the EOs were toxic to the different larval stages. According to the Finney method, concentrations 2.88 and 1.07% are the LC50 of M. rotundifolia and C. coronarium oils, which induce 50% mortality of T. absoluta within 7 days of exposure. Statistical analysis of in-vitro tests showed that both EOs had a similar level of insecticidal efficacy by contact. The overall results showed that the oils used have been shown to have an important insecticidal effect and can be used as a source of biological and natural treatment against tomato leafminer (TLM). |
| format | Article |
| id | doaj-art-9f9a7dfb852b44818c827e3fd5cf44cd |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-9f9a7dfb852b44818c827e3fd5cf44cd2025-01-12T12:20:40ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-025-85606-xChemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absolutaKrache Farial0Boualem Malika1Benourad Fouzia2Touzout Nabil3Mihoub Adil4Subhan Danish5Sulaiman Ali Alharbi6Mohammad Javed Ansari7Abdullah A. Alarfaj8Department of Agronomic Sciences, Institute of Natural and Life Sciences, Morsli Abdellah University CenterPlant Protection Laboratory, Department of Agricultural Sciences, Faculty of Natural and Life Sciences, University Abdelhamid Ibn Badis of MostaganemPlant Protection Laboratory, Department of Agricultural Sciences, Faculty of Natural and Life Sciences, University Abdelhamid Ibn Badis of MostaganemDepartment of Nature and Life Sciences, Faculty of Sciences, Pole Urban Ouzera, University of MedeaBiophysical Environment Station, Center for Scientific and Technical Research on Arid RegionsPesticide Quality Control LaboratoryDepartment of Botany and Microbiology, College of Science, King Saud UniversityDepartment of Botany, Hindu College Moradabad, (Mahatma Jyotiba Phule Rohilkhand University Bareilly) BareillyDepartment of Botany and Microbiology, College of Science, King Saud UniversityAbstract Tuta absoluta is one of the most destructive pests of tomatoes. Chemical insecticides used to control this leafminer harm all organisms, increasing the risk to public health and the environment. Developing natural alternatives, such as bioinsecticides formulated from essential plant oils, is a key strategy to address this problem. These volatile compounds, derived from the secondary metabolic pathways of plants, exhibit targeted activity against specific pest species. Their use is consistent with an environmentally responsible framework that reduces adverse impacts on ecosystems, protects non-target organisms, safeguards human health, and enhances the efficacy of integrated crop management systems. This study aims to determine the chemical composition of the essential oils (EOs) of from round leaf mint (Mentha rotundifolia) and crown chrysanthemum (Chrysanthemum coronarium) and to evaluate their toxicity to T. absoluta larvae in-vitro. The chemical composition of EOs obtained by steam distillation from the leaves of the plants was analyzed by gas chromatography coupled with mass spectrometry (GC-MS). Indeed, 77 volatile compounds representing 98.19% of the total oil of M. rotundifolia, including cyclobutane acetonitrile, 1-methyl-2-(1-methyl ethenyl)-, terpinene-4-ol, p-menthane, germacrene D, caryophyllene and myrcene, were the main compounds. However, farnesene, myrcene, eugenol, germacrene D, phytol, and pinene were significant components among 69 compounds representing 95.39% of the total oil of C. coronarium. Results showed that the EOs were toxic to the different larval stages. According to the Finney method, concentrations 2.88 and 1.07% are the LC50 of M. rotundifolia and C. coronarium oils, which induce 50% mortality of T. absoluta within 7 days of exposure. Statistical analysis of in-vitro tests showed that both EOs had a similar level of insecticidal efficacy by contact. The overall results showed that the oils used have been shown to have an important insecticidal effect and can be used as a source of biological and natural treatment against tomato leafminer (TLM).https://doi.org/10.1038/s41598-025-85606-xTuta absolutaBioinsecticidesMentha rotundifoliaChrysanthemum coronariumEssential oils(GC-MS) |
| spellingShingle | Krache Farial Boualem Malika Benourad Fouzia Touzout Nabil Mihoub Adil Subhan Danish Sulaiman Ali Alharbi Mohammad Javed Ansari Abdullah A. Alarfaj Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta Scientific Reports Tuta absoluta Bioinsecticides Mentha rotundifolia Chrysanthemum coronarium Essential oils (GC-MS) |
| title | Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta |
| title_full | Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta |
| title_fullStr | Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta |
| title_full_unstemmed | Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta |
| title_short | Chemical composition and insecticidal activity of Mentha rotundifolia and Chrysanthemum coronarium essential oils against the tomato leaf miner, Tuta absoluta |
| title_sort | chemical composition and insecticidal activity of mentha rotundifolia and chrysanthemum coronarium essential oils against the tomato leaf miner tuta absoluta |
| topic | Tuta absoluta Bioinsecticides Mentha rotundifolia Chrysanthemum coronarium Essential oils (GC-MS) |
| url | https://doi.org/10.1038/s41598-025-85606-x |
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