Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment
In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating Squarrosus nudus peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its imp...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-11-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624004997 |
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| author | Qianqian Ouyang Yuwei Jiang Lifen Liu Chengfeng Cai Nandika Bandara Puwang Li Kefeng Wu Hui Hong Liang Chen |
| author_facet | Qianqian Ouyang Yuwei Jiang Lifen Liu Chengfeng Cai Nandika Bandara Puwang Li Kefeng Wu Hui Hong Liang Chen |
| author_sort | Qianqian Ouyang |
| collection | DOAJ |
| description | In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating Squarrosus nudus peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its impact on osteogenic activity in vitro. Optimal preparation conditions were determined: a peptide-calcium mass ratio of 5:1, a 30-min reaction time, a temperature of 60 °C, and a pH of 7.0. Under these conditions, a calcium chelating rate of 68.32 % was achieved. Calcium binds to the peptide primarily via carboxyl oxygen and amino nitrogen atoms, and the morphology of SNP-Ca was similar to porous nanoflowers. Our cellular experiments revealed that SNP-Ca significantly increases calcium uptake in Caco-2 cells, stimulating proliferation, differentiation, and mineralization in MC3T3-E1 cells. Additionally, zebrafish larvae models showed enhanced bone formation following SNP-Ca administration. SNP-Ca has the potential of a novel calcium supplement. |
| format | Article |
| id | doaj-art-9e4468c8ae5044fda63f0f77dda88d9c |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-9e4468c8ae5044fda63f0f77dda88d9c2024-11-16T05:10:40ZengElsevierJournal of Functional Foods1756-46462024-11-01122106497Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessmentQianqian Ouyang0Yuwei Jiang1Lifen Liu2Chengfeng Cai3Nandika Bandara4Puwang Li5Kefeng Wu6Hui Hong7Liang Chen8School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, ChinaSchool of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, ChinaDepartment of Food & Human Nutritional Sciences, The University of Manitoba, Canada; Richardson Centre of Food Technology and Research, The University of Manitoba, CanadaSouth Subtropical Crop Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang, Guangdong 524001, China; Corresponding authors.School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Corresponding authors.School of Ocean and Tropical Medicine, Guangdong Medical University, Zhanjiang, Guangdong 524023, China; Corresponding authors.In order to prepare calcium supplements for efficient absorption and utilization in the human body, this study focused on synthesizing SNP-Ca by chelating Squarrosus nudus peptide (SNP) with calcium. We characterized its structure, stability, and calcium uptake properties in Caco-2 cells and its impact on osteogenic activity in vitro. Optimal preparation conditions were determined: a peptide-calcium mass ratio of 5:1, a 30-min reaction time, a temperature of 60 °C, and a pH of 7.0. Under these conditions, a calcium chelating rate of 68.32 % was achieved. Calcium binds to the peptide primarily via carboxyl oxygen and amino nitrogen atoms, and the morphology of SNP-Ca was similar to porous nanoflowers. Our cellular experiments revealed that SNP-Ca significantly increases calcium uptake in Caco-2 cells, stimulating proliferation, differentiation, and mineralization in MC3T3-E1 cells. Additionally, zebrafish larvae models showed enhanced bone formation following SNP-Ca administration. SNP-Ca has the potential of a novel calcium supplement.http://www.sciencedirect.com/science/article/pii/S1756464624004997Peptide-calcium chelateSquarrosus nudusStructural propertiesOsteogenic activity |
| spellingShingle | Qianqian Ouyang Yuwei Jiang Lifen Liu Chengfeng Cai Nandika Bandara Puwang Li Kefeng Wu Hui Hong Liang Chen Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment Journal of Functional Foods Peptide-calcium chelate Squarrosus nudus Structural properties Osteogenic activity |
| title | Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment |
| title_full | Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment |
| title_fullStr | Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment |
| title_full_unstemmed | Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment |
| title_short | Preparation and characterization of Sipunculus nudus peptide-calcium chelate: Structural insights and osteogenic bioactivity assessment |
| title_sort | preparation and characterization of sipunculus nudus peptide calcium chelate structural insights and osteogenic bioactivity assessment |
| topic | Peptide-calcium chelate Squarrosus nudus Structural properties Osteogenic activity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624004997 |
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