Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways

Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic,...

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Main Authors: Hannah L. Klein, Giedrė Bačinskaja, Jun Che, Anais Cheblal, Rajula Elango, Anastasiya Epshtein, Devon M. Fitzgerald, Belén Gómez-González, Sharik R. Khan, Sandeep Kumar, Bryan A. Leland, Léa Marie, Qian Mei, Judith Miné-Hattab, Alicja Piotrowska, Erica J. Polleys, Christopher D. Putnam, Elina A. Radchenko, Anissia Ait Saada, Cynthia J. Sakofsky, Eun Yong Shim, Mathew Stracy, Jun Xia, Zhenxin Yan, Yi Yin, Andrés Aguilera, Juan Lucas Argueso, Catherine H. Freudenreich, Susan M. Gasser, Dmitry A. Gordenin, James E. Haber, Grzegorz Ira, Sue Jinks-Robertson, Megan C. King, Richard D. Kolodner, Andrei Kuzminov, Sarah AE Lambert, Sang Eun Lee, Kyle M. Miller, Sergei M. Mirkin, Thomas D. Petes, Susan M. Rosenberg, Rodney Rothstein, Lorraine S. Symington, Pawel Zawadzki, Nayun Kim, Michael Lisby, Anna Malkova
Format: Article
Language:English
Published: Shared Science Publishers OG 2019-01-01
Series:Microbial Cell
Subjects:
chromatin dynamics
chromosome rearrangements
crossovers
DNA breaks
DNA repair centers
DNA resection
DSBs
gene amplification
gene conversion
genome instability
gross chromosome rearrangements
fluorescent proteins
Holliday junctions
homologous recombination
mitotic recombination
mutagenesis
pulsed field gel electrophoresis
R-loops
single-particle tracking
replication fork stalling
sister repetitive sequences
sister chromatid recombination
site-specific chromosome breaks
toxic recombination intermediates
yeast artificial chromosome
Online Access:http://microbialcell.com/researcharticles/2019a-klein-microbial-cell/
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