Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks

<b>Aim:</b> The purpose of this study was to investigate the plasmid profiles of Enterococcus faecalis isolated from cow milk samples with mastitis.<p> <b>Materials and Methods:</b> As a material, 54 E. faecalis strains isolated in the past years from milk samples with...

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Main Authors: Mehmet Öztürk, Süheyla Türkyılmaz
Format: Article
Language:English
Published: Selcuk University Press
Series:Eurasian Journal of Veterinary Sciences
Subjects:
Online Access:http://eurasianjvetsci.org/pdf.php3?id=1156
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author Mehmet Öztürk
Süheyla Türkyılmaz
author_facet Mehmet Öztürk
Süheyla Türkyılmaz
author_sort Mehmet Öztürk
collection DOAJ
description <b>Aim:</b> The purpose of this study was to investigate the plasmid profiles of Enterococcus faecalis isolated from cow milk samples with mastitis.<p> <b>Materials and Methods:</b> As a material, 54 E. faecalis strains isolated in the past years from milk samples with mastitis were used. Modified alkaline lysis method was used for plasmid isolation from bacteria.<p> <b>Results:</b> Results revealed that 19 of isolates have plasmids varying one to eight and lengths of those were ranged approximately between 0.6 kb and 41 kb. It was determined that five isolates had only one plasmid while the other 14 had more plasmids. Following the plasmid isolation, cluster analysis was performed between the same and similar plasmids after their profile contends checked. Six clusters that containing two or more strains with similar plasmid profile were formed.<p> <b>Conclusions:</b> Further studies should be designed to investigate the plasmids of other enterococci stains isolated from mastitis cow&#8217;s milks whether plasmids are responsible for antibiotic resistance.
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institution Kabale University
issn 1309-6958
2146-1953
language English
publisher Selcuk University Press
record_format Article
series Eurasian Journal of Veterinary Sciences
spelling doaj-art-9d00dc4672fc4865a5f8ecbfdd129a7b2025-01-03T01:36:16ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-195333154591156Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milksMehmet Öztürk0Süheyla Türkyılmaz1Adnan Menderes Üniversitesi, Sağlık Bilimleri Enstitüsü, Aydın, TürkiyeAdnan Menderes Üniversitesi Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Aydın, Türkiye<b>Aim:</b> The purpose of this study was to investigate the plasmid profiles of Enterococcus faecalis isolated from cow milk samples with mastitis.<p> <b>Materials and Methods:</b> As a material, 54 E. faecalis strains isolated in the past years from milk samples with mastitis were used. Modified alkaline lysis method was used for plasmid isolation from bacteria.<p> <b>Results:</b> Results revealed that 19 of isolates have plasmids varying one to eight and lengths of those were ranged approximately between 0.6 kb and 41 kb. It was determined that five isolates had only one plasmid while the other 14 had more plasmids. Following the plasmid isolation, cluster analysis was performed between the same and similar plasmids after their profile contends checked. Six clusters that containing two or more strains with similar plasmid profile were formed.<p> <b>Conclusions:</b> Further studies should be designed to investigate the plasmids of other enterococci stains isolated from mastitis cow&#8217;s milks whether plasmids are responsible for antibiotic resistance.http://eurasianjvetsci.org/pdf.php3?id=1156cowmastitisplasmidenterococcus faecalis
spellingShingle Mehmet Öztürk
Süheyla Türkyılmaz
Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
Eurasian Journal of Veterinary Sciences
cow
mastitis
plasmid
enterococcus faecalis
title Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
title_full Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
title_fullStr Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
title_full_unstemmed Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
title_short Investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
title_sort investigation of plasmid profiles of enterococcus faecalis isolated from mastitic cow milks
topic cow
mastitis
plasmid
enterococcus faecalis
url http://eurasianjvetsci.org/pdf.php3?id=1156
work_keys_str_mv AT mehmetozturk investigationofplasmidprofilesofenterococcusfaecalisisolatedfrommastiticcowmilks
AT suheylaturkyılmaz investigationofplasmidprofilesofenterococcusfaecalisisolatedfrommastiticcowmilks