The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression
Background MiR-381 can regulate the expression of cyclin A2 (CCNA2) to inhibit the proliferation and migration of bladder cancer cells, but whether miR-381 has the same function in breast cancer is not well know.Methods The over express or silence miR-381 expressing cell lines were constructed by le...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Obstetrics and Gynaecology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/01443615.2024.2360547 |
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| author | Ming-Gang Cao Yan Wang Zhi-Min Yang Yang Wang Mei-Qing Wang Shuai Zhuo Yan Yang Chun-Sheng Liu |
| author_facet | Ming-Gang Cao Yan Wang Zhi-Min Yang Yang Wang Mei-Qing Wang Shuai Zhuo Yan Yang Chun-Sheng Liu |
| author_sort | Ming-Gang Cao |
| collection | DOAJ |
| description | Background MiR-381 can regulate the expression of cyclin A2 (CCNA2) to inhibit the proliferation and migration of bladder cancer cells, but whether miR-381 has the same function in breast cancer is not well know.Methods The over express or silence miR-381 expressing cell lines were constructed by lentivirus infection to reveal the biological functions of miR-381 in vitro. The expression of miR-381 and CCNA2 in 162 breast cancer patients were detected to further reveal their impact and predictive value on progression-free survival (PFS) and overall survival (OS).Results After transfection of MDA-MB-231 and MCF-7 cells with miR-381 mimics, the expression of miR-381 was effectively up-regulated and CCNA2 was effectively down-regulated, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. After transfection of cell lines with miR-381 mimics, tumour cell activity was significantly reduced, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. The area under curves (AUCs) of miRNA-381 and CCNA2 for predicting PFS and OS were 0.711, 0.695, 0.694 and 0.675 respectively. Cox regression analysis showed that miRNA-381 ≥ 1.65 2−ΔΔCt and CCNA ≥ 2.95 2−ΔΔCt were the influence factors of PFS and OS, the hazard ratio (HR) values were 0.553, 2.075, 0.462 and 2.089, respectively.Conclusion miR-381 inhibitors breast cancer cells proliferation and migration by down-regulating the expression of CCNA2, both of them can predict the prognosis of breast cancer. |
| format | Article |
| id | doaj-art-9b401e3bc8f8460bbfea8b84bb0b6795 |
| institution | Kabale University |
| issn | 0144-3615 1364-6893 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Obstetrics and Gynaecology |
| spelling | doaj-art-9b401e3bc8f8460bbfea8b84bb0b67952025-01-09T12:13:17ZengTaylor & Francis GroupJournal of Obstetrics and Gynaecology0144-36151364-68932024-12-0144110.1080/01443615.2024.2360547The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expressionMing-Gang Cao0Yan Wang1Zhi-Min Yang2Yang Wang3Mei-Qing Wang4Shuai Zhuo5Yan Yang6Chun-Sheng Liu7Department of Clinical Medicine, Anhui College of Traditional Chinese Medicine, Wuhu, ChinaDepartment of Central Sterile Supply, The Second People’s Hospital of Wuhu, Wuhu, ChinaDepartment of Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaDepartment of Clinical Medicine, Anhui College of Traditional Chinese Medicine, Wuhu, ChinaDepartment of Clinical Medicine, Anhui College of Traditional Chinese Medicine, Wuhu, ChinaDepartment of Clinical Medicine, Anhui College of Traditional Chinese Medicine, Wuhu, ChinaDepartment of Clinical Medicine, Anhui College of Traditional Chinese Medicine, Wuhu, ChinaDepartment of Laboratory, The First Affiliated Hospital of Wannan Medical College, Wuhu, ChinaBackground MiR-381 can regulate the expression of cyclin A2 (CCNA2) to inhibit the proliferation and migration of bladder cancer cells, but whether miR-381 has the same function in breast cancer is not well know.Methods The over express or silence miR-381 expressing cell lines were constructed by lentivirus infection to reveal the biological functions of miR-381 in vitro. The expression of miR-381 and CCNA2 in 162 breast cancer patients were detected to further reveal their impact and predictive value on progression-free survival (PFS) and overall survival (OS).Results After transfection of MDA-MB-231 and MCF-7 cells with miR-381 mimics, the expression of miR-381 was effectively up-regulated and CCNA2 was effectively down-regulated, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. After transfection of cell lines with miR-381 mimics, tumour cell activity was significantly reduced, while the opposite results were observed in tumour cell which transfected with miR-381 inhibitors. The area under curves (AUCs) of miRNA-381 and CCNA2 for predicting PFS and OS were 0.711, 0.695, 0.694 and 0.675 respectively. Cox regression analysis showed that miRNA-381 ≥ 1.65 2−ΔΔCt and CCNA ≥ 2.95 2−ΔΔCt were the influence factors of PFS and OS, the hazard ratio (HR) values were 0.553, 2.075, 0.462 and 2.089, respectively.Conclusion miR-381 inhibitors breast cancer cells proliferation and migration by down-regulating the expression of CCNA2, both of them can predict the prognosis of breast cancer.https://www.tandfonline.com/doi/10.1080/01443615.2024.2360547miR-381cyclin A2breast cancerproliferationmigration |
| spellingShingle | Ming-Gang Cao Yan Wang Zhi-Min Yang Yang Wang Mei-Qing Wang Shuai Zhuo Yan Yang Chun-Sheng Liu The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression Journal of Obstetrics and Gynaecology miR-381 cyclin A2 breast cancer proliferation migration |
| title | The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression |
| title_full | The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression |
| title_fullStr | The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression |
| title_full_unstemmed | The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression |
| title_short | The effect of miR-381 on proliferation and prognosis of breast cancer by altering CCNA2 expression |
| title_sort | effect of mir 381 on proliferation and prognosis of breast cancer by altering ccna2 expression |
| topic | miR-381 cyclin A2 breast cancer proliferation migration |
| url | https://www.tandfonline.com/doi/10.1080/01443615.2024.2360547 |
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