Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme
BackgroundSeveral epidemiological studies have been carried out using Multiple-Locus Variable-number tandem repeat Analysis (MLVA) for M. hyopneumoniae typing. However, a global perspective on the implications of the genetic diversity of this pathogen is lacking.ObjectiveThis study aimed to determin...
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Frontiers Media S.A.
2025-01-01
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| author | Pablo Tamiozzo Virginia García Raúl E. González-Ittig Raúl E. González-Ittig Maria Pieters Maria Pieters Maria Pieters |
| author_facet | Pablo Tamiozzo Virginia García Raúl E. González-Ittig Raúl E. González-Ittig Maria Pieters Maria Pieters Maria Pieters |
| author_sort | Pablo Tamiozzo |
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| description | BackgroundSeveral epidemiological studies have been carried out using Multiple-Locus Variable-number tandem repeat Analysis (MLVA) for M. hyopneumoniae typing. However, a global perspective on the implications of the genetic diversity of this pathogen is lacking.ObjectiveThis study aimed to determine and to analyze the genetic structure of M. hyopneumoniae based on the p97R1-p146R3 MLVA typing scheme and to characterize, analyze and compare MLVA types among countries where the information was publicly available.MethodsA set of 797 publicly available data of M. hyopneumoniaep97R1-p146R3 MLVA genetic types from six different countries were analyzed using Genalex 6.41 software to characterize loci polymorphism and using Structure 2.3.4 software in order to identify the genetic structure.ResultsA total of 185 MLVA types were identified among the analyzed data. For the p97R1 and p146R3 loci, most of the molecular variation in M. hyopneumoniae populations was identified within countries. Three genetic clusters and their recombinations were identified globally.ConclusionM. hyopneumoniae is a genetically diverse pathogen with limited clonality and three well-defined clusters and their combinations were identified in this investigation. The greatest genetic variation of M. hyopneumoniae was observed within countries. |
| format | Article |
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| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-9a907e08355c4dae8c074889c4d62c682025-01-15T14:52:29ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15108251510825Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing schemePablo Tamiozzo0Virginia García1Raúl E. González-Ittig2Raúl E. González-Ittig3Maria Pieters4Maria Pieters5Maria Pieters6Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Córdoba, ArgentinaDepartamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Córdoba, ArgentinaCátedra de Genética de Poblaciones y Evolución, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Córdoba, ArgentinaInstituto de Diversidad y Ecología Animal (IDEA; CONICET-UNC), Córdoba, ArgentinaDepartment of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United StatesVeterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United StatesSwine Disease Eradication Center, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United StatesBackgroundSeveral epidemiological studies have been carried out using Multiple-Locus Variable-number tandem repeat Analysis (MLVA) for M. hyopneumoniae typing. However, a global perspective on the implications of the genetic diversity of this pathogen is lacking.ObjectiveThis study aimed to determine and to analyze the genetic structure of M. hyopneumoniae based on the p97R1-p146R3 MLVA typing scheme and to characterize, analyze and compare MLVA types among countries where the information was publicly available.MethodsA set of 797 publicly available data of M. hyopneumoniaep97R1-p146R3 MLVA genetic types from six different countries were analyzed using Genalex 6.41 software to characterize loci polymorphism and using Structure 2.3.4 software in order to identify the genetic structure.ResultsA total of 185 MLVA types were identified among the analyzed data. For the p97R1 and p146R3 loci, most of the molecular variation in M. hyopneumoniae populations was identified within countries. Three genetic clusters and their recombinations were identified globally.ConclusionM. hyopneumoniae is a genetically diverse pathogen with limited clonality and three well-defined clusters and their combinations were identified in this investigation. The greatest genetic variation of M. hyopneumoniae was observed within countries.https://www.frontiersin.org/articles/10.3389/fvets.2024.1510825/fullMycoplasma hyopneumoniaegenetic structurepanmictic-clonalityMLVAporcine enzootic pneumoniaadmixed ancestry |
| spellingShingle | Pablo Tamiozzo Virginia García Raúl E. González-Ittig Raúl E. González-Ittig Maria Pieters Maria Pieters Maria Pieters Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme Frontiers in Veterinary Science Mycoplasma hyopneumoniae genetic structure panmictic-clonality MLVA porcine enzootic pneumonia admixed ancestry |
| title | Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme |
| title_full | Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme |
| title_fullStr | Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme |
| title_full_unstemmed | Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme |
| title_short | Genetic structure and diversity of Mycoplasma hyopneumoniae based on a MLVA typing scheme |
| title_sort | genetic structure and diversity of mycoplasma hyopneumoniae based on a mlva typing scheme |
| topic | Mycoplasma hyopneumoniae genetic structure panmictic-clonality MLVA porcine enzootic pneumonia admixed ancestry |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1510825/full |
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