Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean
Abstract Proteins in the open ocean represent a significant source of organic matter, and their profiles reflect the metabolic activities of marine microorganisms. Here, by analyzing metaproteomic samples collected from the Pacific, Atlantic and Southern Ocean, we reveal size-fractionated patterns o...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-50867-z |
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| author | Zihao Zhao Chie Amano Thomas Reinthaler Federico Baltar Mónica V. Orellana Gerhard J. Herndl |
| author_facet | Zihao Zhao Chie Amano Thomas Reinthaler Federico Baltar Mónica V. Orellana Gerhard J. Herndl |
| author_sort | Zihao Zhao |
| collection | DOAJ |
| description | Abstract Proteins in the open ocean represent a significant source of organic matter, and their profiles reflect the metabolic activities of marine microorganisms. Here, by analyzing metaproteomic samples collected from the Pacific, Atlantic and Southern Ocean, we reveal size-fractionated patterns of the structure and function of the marine microbiota protein pool in the water column, particularly in the dark ocean (>200 m). Zooplankton proteins contributed three times more than algal proteins to the deep-sea community metaproteome. Gammaproteobacteria exhibited high metabolic activity in the deep-sea, contributing up to 30% of bacterial proteins. Close virus-host interactions of this taxon might explain the dominance of gammaproteobacterial proteins in the dissolved fraction. A high urease expression in nitrifiers suggested links between their dark carbon fixation and zooplankton urea production. In summary, our results uncover the taxonomic contribution of the microbiota to the oceanic protein pool, revealing protein fluxes from particles to the dissolved organic matter pool. |
| format | Article |
| id | doaj-art-97b5abc49bcc4948a4a742f256d9f660 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-97b5abc49bcc4948a4a742f256d9f6602025-01-05T12:34:58ZengNature PortfolioNature Communications2041-17232024-07-0115111510.1038/s41467-024-50867-zMetaproteomic analysis decodes trophic interactions of microorganisms in the dark oceanZihao Zhao0Chie Amano1Thomas Reinthaler2Federico Baltar3Mónica V. Orellana4Gerhard J. Herndl5Department of Functional and Evolutionary Ecology, Bio-Oceanography and Marine Biology Unit, University of ViennaDepartment of Functional and Evolutionary Ecology, Bio-Oceanography and Marine Biology Unit, University of ViennaDepartment of Functional and Evolutionary Ecology, Bio-Oceanography and Marine Biology Unit, University of ViennaDepartment of Functional and Evolutionary Ecology, Bio-Oceanography and Marine Biology Unit, University of ViennaPolar Science Center, Applied Physics Laboratory, University of WashingtonDepartment of Functional and Evolutionary Ecology, Bio-Oceanography and Marine Biology Unit, University of ViennaAbstract Proteins in the open ocean represent a significant source of organic matter, and their profiles reflect the metabolic activities of marine microorganisms. Here, by analyzing metaproteomic samples collected from the Pacific, Atlantic and Southern Ocean, we reveal size-fractionated patterns of the structure and function of the marine microbiota protein pool in the water column, particularly in the dark ocean (>200 m). Zooplankton proteins contributed three times more than algal proteins to the deep-sea community metaproteome. Gammaproteobacteria exhibited high metabolic activity in the deep-sea, contributing up to 30% of bacterial proteins. Close virus-host interactions of this taxon might explain the dominance of gammaproteobacterial proteins in the dissolved fraction. A high urease expression in nitrifiers suggested links between their dark carbon fixation and zooplankton urea production. In summary, our results uncover the taxonomic contribution of the microbiota to the oceanic protein pool, revealing protein fluxes from particles to the dissolved organic matter pool.https://doi.org/10.1038/s41467-024-50867-z |
| spellingShingle | Zihao Zhao Chie Amano Thomas Reinthaler Federico Baltar Mónica V. Orellana Gerhard J. Herndl Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean Nature Communications |
| title | Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| title_full | Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| title_fullStr | Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| title_full_unstemmed | Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| title_short | Metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| title_sort | metaproteomic analysis decodes trophic interactions of microorganisms in the dark ocean |
| url | https://doi.org/10.1038/s41467-024-50867-z |
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