Physical model of serum supplemented medium flow in organ-on-a-chip systems.
Creating a physiologically relevant shear stress in organ-on-a-chip (OOC) devices requires careful tailoring of microfluidic flow parameters. Currently, it is fairly common to use a simple approximation assuming constant viscosity, even for serum-based media. Here, we show that a popular nutrient so...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0322069 |
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| author | Viesturs Šints Jānis Cīmurs Mihails Birjukovs Ivars Driķis Karīna Goluba Kaspars Jēkabsons Vadims Parfejevs Una Riekstiņa Gatis Mozoļevskis Roberts Rimša Guntars Kitenbergs |
| author_facet | Viesturs Šints Jānis Cīmurs Mihails Birjukovs Ivars Driķis Karīna Goluba Kaspars Jēkabsons Vadims Parfejevs Una Riekstiņa Gatis Mozoļevskis Roberts Rimša Guntars Kitenbergs |
| author_sort | Viesturs Šints |
| collection | DOAJ |
| description | Creating a physiologically relevant shear stress in organ-on-a-chip (OOC) devices requires careful tailoring of microfluidic flow parameters. Currently, it is fairly common to use a simple approximation assuming constant viscosity, even for serum-based media. Here, we show that a popular nutrient solution (Dulbecco's Modified Eagle Medium supplemented with Fetal Bovine Serum) requires a more complex treatment (i.e., is a non-Newtonian fluid), with observed shear stress values significantly greater than reported in literature. We measure the rheology of the solutions and combine it with a 3-dimensional flow field measurement to derive shear stress at the channel surface. We verify the experiments with numerical simulations, finding good agreement and deriving flow properties. Finally, we provide relevant expressions for the shear stress approximation, suitable for development of OOC devices with various geometries. |
| format | Article |
| id | doaj-art-978fee788ee94e5b8d52e38c3375c43d |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-978fee788ee94e5b8d52e38c3375c43d2025-08-20T03:47:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032206910.1371/journal.pone.0322069Physical model of serum supplemented medium flow in organ-on-a-chip systems.Viesturs ŠintsJānis CīmursMihails BirjukovsIvars DriķisKarīna GolubaKaspars JēkabsonsVadims ParfejevsUna RiekstiņaGatis MozoļevskisRoberts RimšaGuntars KitenbergsCreating a physiologically relevant shear stress in organ-on-a-chip (OOC) devices requires careful tailoring of microfluidic flow parameters. Currently, it is fairly common to use a simple approximation assuming constant viscosity, even for serum-based media. Here, we show that a popular nutrient solution (Dulbecco's Modified Eagle Medium supplemented with Fetal Bovine Serum) requires a more complex treatment (i.e., is a non-Newtonian fluid), with observed shear stress values significantly greater than reported in literature. We measure the rheology of the solutions and combine it with a 3-dimensional flow field measurement to derive shear stress at the channel surface. We verify the experiments with numerical simulations, finding good agreement and deriving flow properties. Finally, we provide relevant expressions for the shear stress approximation, suitable for development of OOC devices with various geometries.https://doi.org/10.1371/journal.pone.0322069 |
| spellingShingle | Viesturs Šints Jānis Cīmurs Mihails Birjukovs Ivars Driķis Karīna Goluba Kaspars Jēkabsons Vadims Parfejevs Una Riekstiņa Gatis Mozoļevskis Roberts Rimša Guntars Kitenbergs Physical model of serum supplemented medium flow in organ-on-a-chip systems. PLoS ONE |
| title | Physical model of serum supplemented medium flow in organ-on-a-chip systems. |
| title_full | Physical model of serum supplemented medium flow in organ-on-a-chip systems. |
| title_fullStr | Physical model of serum supplemented medium flow in organ-on-a-chip systems. |
| title_full_unstemmed | Physical model of serum supplemented medium flow in organ-on-a-chip systems. |
| title_short | Physical model of serum supplemented medium flow in organ-on-a-chip systems. |
| title_sort | physical model of serum supplemented medium flow in organ on a chip systems |
| url | https://doi.org/10.1371/journal.pone.0322069 |
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