Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization
The sustainable utilization of agricultural by-products for novel protein sources is crucial for addressing global food security. This study investigates the alkaline extraction and characterization of protein isolates from Sacha Inchi (Plukenetia volubilis L.) seed meal, an agro-industrial residue,...
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| Format: | Article |
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Elsevier
2025-10-01
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| Series: | Journal of Agriculture and Food Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666154325006210 |
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| author | Sutharut Jiapong Tippaporn Bualeong Sasivimon Chittrakorn Worasit Tochampa Khanitta Ruttarattanamongkol |
| author_facet | Sutharut Jiapong Tippaporn Bualeong Sasivimon Chittrakorn Worasit Tochampa Khanitta Ruttarattanamongkol |
| author_sort | Sutharut Jiapong |
| collection | DOAJ |
| description | The sustainable utilization of agricultural by-products for novel protein sources is crucial for addressing global food security. This study investigates the alkaline extraction and characterization of protein isolates from Sacha Inchi (Plukenetia volubilis L.) seed meal, an agro-industrial residue, using Response Surface Methodology (RSM). A Box-Behnken design was employed to optimize protein extraction, evaluating the effects of pH (10.5–11.5), temperature (30–60 °C), and extraction time (30–60 min) on protein content, yield, and recovery. Optimal conditions (pH 11.5, 60 °C, 30 min extraction time) yielded protein isolates with a significantly enhanced protein content of 92.36 %, demonstrating the efficacy of the optimized extraction process. Statistical models exhibited high predictive accuracy (R2 = 0.9056–0.9882). Characterization of the isolates revealed improved in vitro digestibility (69.71 % compared to 26.85 % for the seed meal), reduced levels of antinutritional factors, and enhanced functional properties, including emulsion stability and foaming capacity, relevant for food applications. Liquid chromatography-mass spectrometry (LC/MS) analysis identified Legumin A2 as the predominant protein, with molecular weights in the range of 21–62 kDa. The amino acid profile highlighted substantial levels of essential amino acids, especially branched-chain amino acids, although lysine and tryptophan were identified as limiting amino acids relative to FAO/WHO standards. This research establishes a scalable and efficient methodology for extracting high-quality protein isolates from Sacha Inchi seed meal, positioning it as a valuable, sustainable ingredient for food product development and agricultural waste valorization. |
| format | Article |
| id | doaj-art-97797c0a500a4ba0a793814ae3ce5923 |
| institution | Kabale University |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-97797c0a500a4ba0a793814ae3ce59232025-08-20T03:59:37ZengElsevierJournal of Agriculture and Food Research2666-15432025-10-012310225010.1016/j.jafr.2025.102250Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterizationSutharut Jiapong0Tippaporn Bualeong1Sasivimon Chittrakorn2Worasit Tochampa3Khanitta Ruttarattanamongkol4Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, ThailandDepartment of Physiology, Faculty of Medical Science, Naresuan University, Phitsanulok, ThailandDepartment of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, ThailandDepartment of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, ThailandDepartment of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand; Center of Excellence for Fats and Oils, Naresuan University, Phitsanulok, Thailand; Center of Excellence for Natural Health Product Innovation, Thailand; Corresponding author. Department of Agro-Industry, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok, Thailand.The sustainable utilization of agricultural by-products for novel protein sources is crucial for addressing global food security. This study investigates the alkaline extraction and characterization of protein isolates from Sacha Inchi (Plukenetia volubilis L.) seed meal, an agro-industrial residue, using Response Surface Methodology (RSM). A Box-Behnken design was employed to optimize protein extraction, evaluating the effects of pH (10.5–11.5), temperature (30–60 °C), and extraction time (30–60 min) on protein content, yield, and recovery. Optimal conditions (pH 11.5, 60 °C, 30 min extraction time) yielded protein isolates with a significantly enhanced protein content of 92.36 %, demonstrating the efficacy of the optimized extraction process. Statistical models exhibited high predictive accuracy (R2 = 0.9056–0.9882). Characterization of the isolates revealed improved in vitro digestibility (69.71 % compared to 26.85 % for the seed meal), reduced levels of antinutritional factors, and enhanced functional properties, including emulsion stability and foaming capacity, relevant for food applications. Liquid chromatography-mass spectrometry (LC/MS) analysis identified Legumin A2 as the predominant protein, with molecular weights in the range of 21–62 kDa. The amino acid profile highlighted substantial levels of essential amino acids, especially branched-chain amino acids, although lysine and tryptophan were identified as limiting amino acids relative to FAO/WHO standards. This research establishes a scalable and efficient methodology for extracting high-quality protein isolates from Sacha Inchi seed meal, positioning it as a valuable, sustainable ingredient for food product development and agricultural waste valorization.http://www.sciencedirect.com/science/article/pii/S2666154325006210Sacha Inchi protein isolationResponse surface methodologyProtein extraction optimizationFunctional food ingredientsAgricultural by-product utilizationAmino acid profile |
| spellingShingle | Sutharut Jiapong Tippaporn Bualeong Sasivimon Chittrakorn Worasit Tochampa Khanitta Ruttarattanamongkol Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization Journal of Agriculture and Food Research Sacha Inchi protein isolation Response surface methodology Protein extraction optimization Functional food ingredients Agricultural by-product utilization Amino acid profile |
| title | Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization |
| title_full | Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization |
| title_fullStr | Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization |
| title_full_unstemmed | Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization |
| title_short | Optimized protein isolation from industrial Sacha Inchi seed meal via alkaline extraction: Functional and nutritional characterization |
| title_sort | optimized protein isolation from industrial sacha inchi seed meal via alkaline extraction functional and nutritional characterization |
| topic | Sacha Inchi protein isolation Response surface methodology Protein extraction optimization Functional food ingredients Agricultural by-product utilization Amino acid profile |
| url | http://www.sciencedirect.com/science/article/pii/S2666154325006210 |
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