Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer
Malignant tumors remain a serious threat to human health and life and are a major public health problem globally. Herein, we designed and synthesized a novel nucleic acid nanomedicine AS1411-siRNA-LNPs (As@LNPs). Bmi-1 siRNA was coated with cationic liposomes, and a nucleic acid aptamer AS1411 with...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-09-01
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| Series: | Nano Biomedicine and Engineering |
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| Online Access: | https://www.sciopen.com/article/10.26599/NBE.2024.9290086 |
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| author | Hongzhang Yan Hong Shen Jinrong SiTu Yingying Yang Lingle Zhang Kai Yang |
| author_facet | Hongzhang Yan Hong Shen Jinrong SiTu Yingying Yang Lingle Zhang Kai Yang |
| author_sort | Hongzhang Yan |
| collection | DOAJ |
| description | Malignant tumors remain a serious threat to human health and life and are a major public health problem globally. Herein, we designed and synthesized a novel nucleic acid nanomedicine AS1411-siRNA-LNPs (As@LNPs). Bmi-1 siRNA was coated with cationic liposomes, and a nucleic acid aptamer AS1411 with tumor cell-targeting ability was attached to the outermost layer of the liposomes. The average particle size of As@LNPs was 183 nm, and the polydispersion coefficient was 0.187. The encapsulation rate and drug loading of As@LNPs were 85% and 4.6%, respectively. The average electron mobility of the drug was 2.64 (μ/s)/(V/cm), and the zeta potential of As@LNPs was 33.79 ± 0.78 mV. The microstructure of the nanomedicine was evaluated via transmission electron microscopy. In vitro and in vivo experiments showed that As@LNPs significantly inhibited tumor growth and promoted tumor cell apoptosis. As@LNPs showed favorable biosafety with major tissues and organs, except glomerulus and renal epithelial cells. |
| format | Article |
| id | doaj-art-969a76f9b03e4cab8ecb5298f3ea98e3 |
| institution | Kabale University |
| issn | 2097-3837 2150-5578 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Nano Biomedicine and Engineering |
| spelling | doaj-art-969a76f9b03e4cab8ecb5298f3ea98e32024-11-11T04:02:04ZengTsinghua University PressNano Biomedicine and Engineering2097-38372150-55782024-09-0116341642810.26599/NBE.2024.9290086Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric CancerHongzhang Yan0Hong Shen1Jinrong SiTu2Yingying Yang3Lingle Zhang4Kai Yang5College of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaCollege of Fisheries and Life Science of Shanghai Ocean University, Shanghai 201306, ChinaMalignant tumors remain a serious threat to human health and life and are a major public health problem globally. Herein, we designed and synthesized a novel nucleic acid nanomedicine AS1411-siRNA-LNPs (As@LNPs). Bmi-1 siRNA was coated with cationic liposomes, and a nucleic acid aptamer AS1411 with tumor cell-targeting ability was attached to the outermost layer of the liposomes. The average particle size of As@LNPs was 183 nm, and the polydispersion coefficient was 0.187. The encapsulation rate and drug loading of As@LNPs were 85% and 4.6%, respectively. The average electron mobility of the drug was 2.64 (μ/s)/(V/cm), and the zeta potential of As@LNPs was 33.79 ± 0.78 mV. The microstructure of the nanomedicine was evaluated via transmission electron microscopy. In vitro and in vivo experiments showed that As@LNPs significantly inhibited tumor growth and promoted tumor cell apoptosis. As@LNPs showed favorable biosafety with major tissues and organs, except glomerulus and renal epithelial cells.https://www.sciopen.com/article/10.26599/NBE.2024.9290086malignant tumorcationic liposomeas@lnpssirnabmi-1drug delivery |
| spellingShingle | Hongzhang Yan Hong Shen Jinrong SiTu Yingying Yang Lingle Zhang Kai Yang Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer Nano Biomedicine and Engineering malignant tumor cationic liposome as@lnps sirna bmi-1 drug delivery |
| title | Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer |
| title_full | Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer |
| title_fullStr | Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer |
| title_full_unstemmed | Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer |
| title_short | Preparation of Bmi-1-siRNA Lipid Nanoparticles and Effects in Gastric Cancer |
| title_sort | preparation of bmi 1 sirna lipid nanoparticles and effects in gastric cancer |
| topic | malignant tumor cationic liposome as@lnps sirna bmi-1 drug delivery |
| url | https://www.sciopen.com/article/10.26599/NBE.2024.9290086 |
| work_keys_str_mv | AT hongzhangyan preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer AT hongshen preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer AT jinrongsitu preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer AT yingyingyang preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer AT linglezhang preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer AT kaiyang preparationofbmi1sirnalipidnanoparticlesandeffectsingastriccancer |