分枝杆菌利福平耐药基因<italic>rpoB</italic>高通量突变文库的构建
目的建立利福平耐药基因<italic>rpoB</italic>高通量突变文库。方法通过同源重组和L5<italic> attB</italic>噬菌体整合位点交换构建耻垢分枝杆菌Δ<italic>rpoB</italic> <italic>attB</italic>：：<italic>rpoB</italic>菌株；基于L5<italic> attB</italic>质粒交换系统和抗性选择培养基，挑取48个克隆以验证质粒替换的效率；针对利福平耐药决定...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2023-07-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/doi/10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2023.0413/ |
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