Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Accelerated cGMP production of near-native HIV-1 Env trimers following electroporation transfection and immunogenicity analysis

Abstract Evaluation of recombinant HIV-1 surface glycoproteins (Env) as vaccine candidates for Phase I human experimental trials often requires production of cGMP-grade well-ordered Env trimers. Here, we report an accelerated cGMP compatible approach for expression and purification of a stabilized H...

Full description

Saved in:
Bibliographic Details
Main Authors: Shridhar Bale, Elena Gustchina, Javier Guenaga, Victor Ayala, Wen-Hsin Lee, Gabriel Ozorowski, Stephen Whitney, Richard Wilson, Sabyasachi Baboo, Jolene K. Diedrich, Esmeralda D. Doyle, Lauren Hudacik, Elana Ben-Akiva, Kristen A. Rodrigues, Darrell J. Irvine, John R. Yates, James C. Paulson, Andrew B. Ward, Timothy Fouts, Richard T. Wyatt
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:npj Vaccines
Online Access:https://doi.org/10.1038/s41541-025-01218-6
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

https://doi.org/10.1038/s41541-025-01218-6

Similar Items