The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs
Abstract Sepsis leads to an acute breakdown of muscle to support increased caloric and amino acid requirements. Little is known about the role of adipose and muscle tissue breakdown and intestinal metabolism in glucose substrate supply during the acute phase of sepsis. In a translational porcine mod...
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2025-01-01
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| Online Access: | https://doi.org/10.14814/phy2.70129 |
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| author | Ryan Morse Gabriella A. M. Ten Have John J. Thaden Marielle P. K. J. Engelen Sarah Rice Martin Hagve Nicolaas E. P. Deutz |
| author_facet | Ryan Morse Gabriella A. M. Ten Have John J. Thaden Marielle P. K. J. Engelen Sarah Rice Martin Hagve Nicolaas E. P. Deutz |
| author_sort | Ryan Morse |
| collection | DOAJ |
| description | Abstract Sepsis leads to an acute breakdown of muscle to support increased caloric and amino acid requirements. Little is known about the role of adipose and muscle tissue breakdown and intestinal metabolism in glucose substrate supply during the acute phase of sepsis. In a translational porcine model of sepsis, we explored the across organ net fluxes of gluconeogenic substrates. In 13 pigs, acute sepsis was induced by IV infusion of Pseudomonas aeruginosa, while in 9 pigs saline (control) was given for 18 h. Blood samples were collected between 12 and 18 h and analyzed with HPLC and LCMS. In sepsis, glucose plasma concentration was reduced (p = 0.0028). A concordant increase in splanchnic area net release of glucose (p = 0.0049), due to reduced uptake in the portal drained viscera (PDV) (p = 0.0032) with an unchanged liver production (p = 0.7861). The hindquarter showed a higher release of alanine (p = 0.0002), glutamine (p = 0.003), and lactate (p = 0.0007), but not for glycerol (p = 0.5718). Diminished PDV uptake of gluconeogenic amino acids, increased liver uptake of these substrates (p < 0.05), while no change in liver glycerol uptake (p = 0.3170), did not lead to an increased net liver glucose release. In the acute phase of sepsis, we hypothesize an important role of altered intestinal amino acid metabolism and breakdown of muscle proteins, but not of glycolysis to support gluconeogenesis. |
| format | Article |
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| institution | Kabale University |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| series | Physiological Reports |
| spelling | doaj-art-95a6d3dc2f09485a9ac4c934aa5ece662025-01-15T13:36:31ZengWileyPhysiological Reports2051-817X2025-01-01131n/an/a10.14814/phy2.70129The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigsRyan Morse0Gabriella A. M. Ten Have1John J. Thaden2Marielle P. K. J. Engelen3Sarah Rice4Martin Hagve5Nicolaas E. P. Deutz6Center for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USACenter for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USACenter for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USACenter for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USACenter for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USADepartment of Gastrointestinal Surgery University Hospital North‐Norway Tromso NorwayCenter for Translational Research in Aging and Longevity, Department of Health and Kinesiology Texas A&M University College Station Texas USAAbstract Sepsis leads to an acute breakdown of muscle to support increased caloric and amino acid requirements. Little is known about the role of adipose and muscle tissue breakdown and intestinal metabolism in glucose substrate supply during the acute phase of sepsis. In a translational porcine model of sepsis, we explored the across organ net fluxes of gluconeogenic substrates. In 13 pigs, acute sepsis was induced by IV infusion of Pseudomonas aeruginosa, while in 9 pigs saline (control) was given for 18 h. Blood samples were collected between 12 and 18 h and analyzed with HPLC and LCMS. In sepsis, glucose plasma concentration was reduced (p = 0.0028). A concordant increase in splanchnic area net release of glucose (p = 0.0049), due to reduced uptake in the portal drained viscera (PDV) (p = 0.0032) with an unchanged liver production (p = 0.7861). The hindquarter showed a higher release of alanine (p = 0.0002), glutamine (p = 0.003), and lactate (p = 0.0007), but not for glycerol (p = 0.5718). Diminished PDV uptake of gluconeogenic amino acids, increased liver uptake of these substrates (p < 0.05), while no change in liver glycerol uptake (p = 0.3170), did not lead to an increased net liver glucose release. In the acute phase of sepsis, we hypothesize an important role of altered intestinal amino acid metabolism and breakdown of muscle proteins, but not of glycolysis to support gluconeogenesis.https://doi.org/10.14814/phy2.70129amino acid metabolismgluconeogenesisglucoselipid metabolismpigsepsis |
| spellingShingle | Ryan Morse Gabriella A. M. Ten Have John J. Thaden Marielle P. K. J. Engelen Sarah Rice Martin Hagve Nicolaas E. P. Deutz The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs Physiological Reports amino acid metabolism gluconeogenesis glucose lipid metabolism pig sepsis |
| title | The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs |
| title_full | The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs |
| title_fullStr | The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs |
| title_full_unstemmed | The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs |
| title_short | The role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a Pseudomonas aeruginosa induced sepsis model in female pigs |
| title_sort | role of adipose and muscle tissue breakdown on interorgan energy substrate fluxes in a pseudomonas aeruginosa induced sepsis model in female pigs |
| topic | amino acid metabolism gluconeogenesis glucose lipid metabolism pig sepsis |
| url | https://doi.org/10.14814/phy2.70129 |
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