Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography
Methods for tagging biomolecules with fluorine 18 as immuno–positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2011-05-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2010.00043 |
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| _version_ | 1841561868546605056 |
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| author | Kan Liu Eric J. Lepin Ming-Wei Wang Feng Guo Wei-Yu Lin Yi-Chun Chen Shannon J. Sirk Sebastian Olma Michael E. Phelps Xing-Zhong Zhao Hsian-Rong Tseng R. Michael van Dam Anna M. Wu Clifton K.-F. Shen |
| author_facet | Kan Liu Eric J. Lepin Ming-Wei Wang Feng Guo Wei-Yu Lin Yi-Chun Chen Shannon J. Sirk Sebastian Olma Michael E. Phelps Xing-Zhong Zhao Hsian-Rong Tseng R. Michael van Dam Anna M. Wu Clifton K.-F. Shen |
| author_sort | Kan Liu |
| collection | DOAJ |
| description | Methods for tagging biomolecules with fluorine 18 as immuno–positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18 F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18 F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection. |
| format | Article |
| id | doaj-art-937eccc6059a4a5281f726e83f30f961 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2011-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-937eccc6059a4a5281f726e83f30f9612025-01-03T01:22:54ZengSAGE PublishingMolecular Imaging1536-01212011-05-011010.2310/7290.2010.0004310.2310_7290.2010.00043Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission TomographyKan LiuEric J. LepinMing-Wei WangFeng GuoWei-Yu LinYi-Chun ChenShannon J. SirkSebastian OlmaMichael E. PhelpsXing-Zhong ZhaoHsian-Rong TsengR. Michael van DamAnna M. WuClifton K.-F. ShenMethods for tagging biomolecules with fluorine 18 as immuno–positron emission tomography (immunoPET) tracers require tedious optimization of radiolabeling conditions and can consume large amounts of scarce biomolecules. We describe an improved method using a digital microfluidic droplet generation (DMDG) chip, which provides computer-controlled metering and mixing of 18 F tag, biomolecule, and buffer in defined ratios, allowing rapid scouting of reaction conditions in nanoliter volumes. The identified optimized conditions were then translated to bench-scale 18 F labeling of a cancer-specific engineered antibody fragments, enabling microPET imaging of tumors in xenografted mice at 0.5 to 4 hours postinjection.https://doi.org/10.2310/7290.2010.00043 |
| spellingShingle | Kan Liu Eric J. Lepin Ming-Wei Wang Feng Guo Wei-Yu Lin Yi-Chun Chen Shannon J. Sirk Sebastian Olma Michael E. Phelps Xing-Zhong Zhao Hsian-Rong Tseng R. Michael van Dam Anna M. Wu Clifton K.-F. Shen Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography Molecular Imaging |
| title | Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography |
| title_full | Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography |
| title_fullStr | Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography |
| title_full_unstemmed | Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography |
| title_short | Microfluidic-Based F-Labeling of Biomolecules for Immuno–Positron Emission Tomography |
| title_sort | microfluidic based f labeling of biomolecules for immuno positron emission tomography |
| url | https://doi.org/10.2310/7290.2010.00043 |
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