A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont
The impact of combined heat and drought stress was investigated in Arabidopsis thaliana and compared to individual stresses to reveal additive effects and interactions. A combination of plant metabolomics and root and rhizosphere bacterial metabarcoding were used to unravel effects at the plant holo...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1484251/full |
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| _version_ | 1846116334956445696 |
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| author | Biancamaria Senizza Fabrizio Araniti Simon Lewin Sonja Wende Steffen Kolb Steffen Kolb Luigi Lucini |
| author_facet | Biancamaria Senizza Fabrizio Araniti Simon Lewin Sonja Wende Steffen Kolb Steffen Kolb Luigi Lucini |
| author_sort | Biancamaria Senizza |
| collection | DOAJ |
| description | The impact of combined heat and drought stress was investigated in Arabidopsis thaliana and compared to individual stresses to reveal additive effects and interactions. A combination of plant metabolomics and root and rhizosphere bacterial metabarcoding were used to unravel effects at the plant holobiont level. Hierarchical cluster analysis of metabolomics signatures pointed out two main clusters, one including heat and combined heat and drought, and the second cluster that included the control and drought treatments. Overall, phenylpropanoids and nitrogen-containing compounds, hormones and amino acids showed the highest discriminant potential. A decrease in alpha-diversity of Bacteria was observed upon stress, with stress-dependent differences in bacterial microbiota composition. The shift in beta-diversity highlighted the pivotal enrichment of Proteobacteria, including Rhizobiales, Enterobacteriales and Azospirillales. The results corroborate the concept of stress interaction, where the combined heat and drought stress is not the mere combination of the single stresses. Intriguingly, multi-omics interpretations evidenced a good correlation between root metabolomics and root bacterial microbiota, indicating an orchestrated modulation of the whole holobiont. |
| format | Article |
| id | doaj-art-935e12ab19224bffa43aa4e4f0cacdd8 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-935e12ab19224bffa43aa4e4f0cacdd82024-12-19T06:24:02ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.14842511484251A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiontBiancamaria Senizza0Fabrizio Araniti1Simon Lewin2Sonja Wende3Steffen Kolb4Steffen Kolb5Luigi Lucini6Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, Piacenza, ItalyDipartimento di Scienze Agrarie e Ambientali, Produzione, Territorio, Agroenergia (Di.S.A.A.), Università degli Studi di Milano, Milano, ItalyMicrobial Biogeochemistry, Research Area Landscape Functioning, Leibniz Center for Agricultural Landscape Research – Leibniz Center for Agricultural Landscape Research e.V. (ZALF), Muencheberg, GermanyMicrobial Biogeochemistry, Research Area Landscape Functioning, Leibniz Center for Agricultural Landscape Research – Leibniz Center for Agricultural Landscape Research e.V. (ZALF), Muencheberg, GermanyMicrobial Biogeochemistry, Research Area Landscape Functioning, Leibniz Center for Agricultural Landscape Research – Leibniz Center for Agricultural Landscape Research e.V. (ZALF), Muencheberg, GermanyThaer Institute, Faculty of Life Sciences, Humboldt University of Berlin, Berlin, GermanyDepartment for Sustainable Food Process, Università Cattolica del Sacro Cuore, Piacenza, ItalyThe impact of combined heat and drought stress was investigated in Arabidopsis thaliana and compared to individual stresses to reveal additive effects and interactions. A combination of plant metabolomics and root and rhizosphere bacterial metabarcoding were used to unravel effects at the plant holobiont level. Hierarchical cluster analysis of metabolomics signatures pointed out two main clusters, one including heat and combined heat and drought, and the second cluster that included the control and drought treatments. Overall, phenylpropanoids and nitrogen-containing compounds, hormones and amino acids showed the highest discriminant potential. A decrease in alpha-diversity of Bacteria was observed upon stress, with stress-dependent differences in bacterial microbiota composition. The shift in beta-diversity highlighted the pivotal enrichment of Proteobacteria, including Rhizobiales, Enterobacteriales and Azospirillales. The results corroborate the concept of stress interaction, where the combined heat and drought stress is not the mere combination of the single stresses. Intriguingly, multi-omics interpretations evidenced a good correlation between root metabolomics and root bacterial microbiota, indicating an orchestrated modulation of the whole holobiont.https://www.frontiersin.org/articles/10.3389/fpls.2024.1484251/fullheat stressdroughtmulti-omicsphytohormonesProteobacteriaRhizobiales |
| spellingShingle | Biancamaria Senizza Fabrizio Araniti Simon Lewin Sonja Wende Steffen Kolb Steffen Kolb Luigi Lucini A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont Frontiers in Plant Science heat stress drought multi-omics phytohormones Proteobacteria Rhizobiales |
| title | A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont |
| title_full | A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont |
| title_fullStr | A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont |
| title_full_unstemmed | A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont |
| title_short | A multi-omics approach to unravel the interaction between heat and drought stress in the Arabidopsis thaliana holobiont |
| title_sort | multi omics approach to unravel the interaction between heat and drought stress in the arabidopsis thaliana holobiont |
| topic | heat stress drought multi-omics phytohormones Proteobacteria Rhizobiales |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1484251/full |
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