Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue
It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/234178 |
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| author | Chi-Fang Peng Xiao-Hui Duan Qiu-Li Pan Li-Qiang Liu Feng Xue |
| author_facet | Chi-Fang Peng Xiao-Hui Duan Qiu-Li Pan Li-Qiang Liu Feng Xue |
| author_sort | Chi-Fang Peng |
| collection | DOAJ |
| description | It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA). The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold. The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer. This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants. |
| format | Article |
| id | doaj-art-931722dbfd5d46af8a45e410540a96c0 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-931722dbfd5d46af8a45e410540a96c02025-02-03T05:47:49ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/234178234178Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken TissueChi-Fang Peng0Xiao-Hui Duan1Qiu-Li Pan2Li-Qiang Liu3Feng Xue4State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaAnimal, Plant & Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 200001, ChinaIt is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues. Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA). The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold. The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer. This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants.http://dx.doi.org/10.1155/2013/234178 |
| spellingShingle | Chi-Fang Peng Xiao-Hui Duan Qiu-Li Pan Li-Qiang Liu Feng Xue Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue Journal of Chemistry |
| title | Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue |
| title_full | Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue |
| title_fullStr | Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue |
| title_full_unstemmed | Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue |
| title_short | Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue |
| title_sort | ultrasensitive nano elisa for detecting sulfadimethoxine in chicken tissue |
| url | http://dx.doi.org/10.1155/2013/234178 |
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