Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis
Patient loss to follow-up caused by centralised and expensive diagnostics that are reliant on sputum is a major obstacle in the fight to end tuberculosis. An affordable, non-sputum biomarker-based, point-of-care deployable test is needed to address this. Serum antibodies binding the mycobacterial ce...
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MDPI AG
2024-11-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/9/11/269 |
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| author | Alma Truyts Ilse Du Preez Eldas M. Maesela Manfred R. Scriba Les Baillie Arwyn T. Jones Kevin J. Land Jan A. Verschoor Yolandy Lemmer |
| author_facet | Alma Truyts Ilse Du Preez Eldas M. Maesela Manfred R. Scriba Les Baillie Arwyn T. Jones Kevin J. Land Jan A. Verschoor Yolandy Lemmer |
| author_sort | Alma Truyts |
| collection | DOAJ |
| description | Patient loss to follow-up caused by centralised and expensive diagnostics that are reliant on sputum is a major obstacle in the fight to end tuberculosis. An affordable, non-sputum biomarker-based, point-of-care deployable test is needed to address this. Serum antibodies binding the mycobacterial cell wall lipids, mycolic acids, have shown promise as biomarkers for active tuberculosis. However, anti-lipid antibodies are of low affinity, making them difficult to detect in a lateral flow immunoassay—a technology widely deployed at the point-of-care. Previously, recombinant monoclonal anti-mycolate antibodies were developed and applied to characterise the antigenicity of mycolic acid. We now demonstrate that these anti-mycolate antibodies specifically detect hexane extracts of mycobacteria. Secondary antibody-mediated detection was applied to detect the displacement of the monoclonal mycolate antibodies by the anti-mycolic acid antibodies present in tuberculosis-positive guinea pig and human serum samples. These data establish proof-of-concept for a novel lateral flow immunoassay for tuberculosis provisionally named MALIA—mycolate antibody lateral flow immunoassay. |
| format | Article |
| id | doaj-art-92ea0ca414d0423da8cbb9f8f11a6095 |
| institution | Kabale University |
| issn | 2414-6366 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj-art-92ea0ca414d0423da8cbb9f8f11a60952024-11-26T18:24:15ZengMDPI AGTropical Medicine and Infectious Disease2414-63662024-11-0191126910.3390/tropicalmed9110269Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of TuberculosisAlma Truyts0Ilse Du Preez1Eldas M. Maesela2Manfred R. Scriba3Les Baillie4Arwyn T. Jones5Kevin J. Land6Jan A. Verschoor7Yolandy Lemmer8Future Production: Chemicals, Council for Scientific and Industrial Research, Pretoria 0081, South AfricaFuture Production: Chemicals, Council for Scientific and Industrial Research, Pretoria 0081, South AfricaFuture Production: Chemicals, Council for Scientific and Industrial Research, Pretoria 0081, South AfricaFuture Production: Chemicals, Council for Scientific and Industrial Research, Pretoria 0081, South AfricaSchool of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff CF10 3NB, UKSchool of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff CF10 3NB, UKGlobal Access Diagnostics, Thurleigh, Bedford MK44 2YA, UKDepartment of Biochemistry, Genetics and Microbiology, University of Pretoria, Pretoria 0002, South AfricaFuture Production: Chemicals, Council for Scientific and Industrial Research, Pretoria 0081, South AfricaPatient loss to follow-up caused by centralised and expensive diagnostics that are reliant on sputum is a major obstacle in the fight to end tuberculosis. An affordable, non-sputum biomarker-based, point-of-care deployable test is needed to address this. Serum antibodies binding the mycobacterial cell wall lipids, mycolic acids, have shown promise as biomarkers for active tuberculosis. However, anti-lipid antibodies are of low affinity, making them difficult to detect in a lateral flow immunoassay—a technology widely deployed at the point-of-care. Previously, recombinant monoclonal anti-mycolate antibodies were developed and applied to characterise the antigenicity of mycolic acid. We now demonstrate that these anti-mycolate antibodies specifically detect hexane extracts of mycobacteria. Secondary antibody-mediated detection was applied to detect the displacement of the monoclonal mycolate antibodies by the anti-mycolic acid antibodies present in tuberculosis-positive guinea pig and human serum samples. These data establish proof-of-concept for a novel lateral flow immunoassay for tuberculosis provisionally named MALIA—mycolate antibody lateral flow immunoassay.https://www.mdpi.com/2414-6366/9/11/269tuberculosisnon-sputum-based diagnosispoint-of-carelateral flow immunoassaybiomarkermycolic acid |
| spellingShingle | Alma Truyts Ilse Du Preez Eldas M. Maesela Manfred R. Scriba Les Baillie Arwyn T. Jones Kevin J. Land Jan A. Verschoor Yolandy Lemmer Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis Tropical Medicine and Infectious Disease tuberculosis non-sputum-based diagnosis point-of-care lateral flow immunoassay biomarker mycolic acid |
| title | Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis |
| title_full | Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis |
| title_fullStr | Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis |
| title_full_unstemmed | Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis |
| title_short | Application of Monoclonal Anti-Mycolate Antibodies in Serological Diagnosis of Tuberculosis |
| title_sort | application of monoclonal anti mycolate antibodies in serological diagnosis of tuberculosis |
| topic | tuberculosis non-sputum-based diagnosis point-of-care lateral flow immunoassay biomarker mycolic acid |
| url | https://www.mdpi.com/2414-6366/9/11/269 |
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