Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells
Objective:To investigate the effects of Stroke-Ⅱoral liquid on TLR4/MyD88/NF-κB signal pathway in chemical damaged PC12 cells.Methods:The chemical damaged PC12 cells model was established and treated by Stroke-Ⅱdrug-containing serum. All cells were divided into blank control group, model group, low...
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| Format: | Article |
| Language: | English |
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Editorial Office of Rehabilitation Medicine
2016-12-01
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| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2016.06028 |
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| _version_ | 1841536956238921728 |
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| author | Xinfu LIN Ling SHI Hengzhao ZHU Sujuan CHEN Xuehua ZHENG Yiman WU |
| author_facet | Xinfu LIN Ling SHI Hengzhao ZHU Sujuan CHEN Xuehua ZHENG Yiman WU |
| author_sort | Xinfu LIN |
| collection | DOAJ |
| description | Objective:To investigate the effects of Stroke-Ⅱoral liquid on TLR4/MyD88/NF-κB signal pathway in chemical damaged PC12 cells.Methods:The chemical damaged PC12 cells model was established and treated by Stroke-Ⅱdrug-containing serum. All cells were divided into blank control group, model group, low dose group and high dose group. The cell proliferation was observed by MTT assay, the expression of TLR4, MyD88 and NF-κB mRNA were detected by RT-PCR, and their protein expressions were detected by Western Blot.Results:Compared with the blank control group, the proliferation of PC12 cells in the model group was decreased (<italic>P</italic><0.01), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were increased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05). Compared with the model group, the proliferation of PC12 cells in low dose group and high dose group were increased (<italic>P</italic><0.01, <italic>P</italic><0.05), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were decreased (<italic>P</italic><0.01, <italic>P</italic><0.05). Compared with the low dose group, the proliferation of PC12 cells in the high dose group was increased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were decreased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05).Conclusion:Stroke-Ⅱoral liquid could protect against chemical damage in PC12 cells via suppression of TLR4/MyD88/NF-κB signal pathway to regulate the inflammatory cascade response. |
| format | Article |
| id | doaj-art-9091e27ecdbd42df9e5a4770dc16f3a4 |
| institution | Kabale University |
| issn | 2096-0328 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Editorial Office of Rehabilitation Medicine |
| record_format | Article |
| series | 康复学报 |
| spelling | doaj-art-9091e27ecdbd42df9e5a4770dc16f3a42025-01-14T10:06:02ZengEditorial Office of Rehabilitation Medicine康复学报2096-03282016-12-0126283323096776Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 CellsXinfu LINLing SHIHengzhao ZHUSujuan CHENXuehua ZHENGYiman WUObjective:To investigate the effects of Stroke-Ⅱoral liquid on TLR4/MyD88/NF-κB signal pathway in chemical damaged PC12 cells.Methods:The chemical damaged PC12 cells model was established and treated by Stroke-Ⅱdrug-containing serum. All cells were divided into blank control group, model group, low dose group and high dose group. The cell proliferation was observed by MTT assay, the expression of TLR4, MyD88 and NF-κB mRNA were detected by RT-PCR, and their protein expressions were detected by Western Blot.Results:Compared with the blank control group, the proliferation of PC12 cells in the model group was decreased (<italic>P</italic><0.01), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were increased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05). Compared with the model group, the proliferation of PC12 cells in low dose group and high dose group were increased (<italic>P</italic><0.01, <italic>P</italic><0.05), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were decreased (<italic>P</italic><0.01, <italic>P</italic><0.05). Compared with the low dose group, the proliferation of PC12 cells in the high dose group was increased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05), and the expression of TLR4, MyD88 and NF-κB mRNA and protein were decreased significantly (<italic>P</italic><0.01, <italic>P</italic><0.05).Conclusion:Stroke-Ⅱoral liquid could protect against chemical damage in PC12 cells via suppression of TLR4/MyD88/NF-κB signal pathway to regulate the inflammatory cascade response.http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2016.06028Stroke-Ⅱoral liquidPC12 cellTLR4/MyD88/NF-κB signal pathwayinflammatory cascade |
| spellingShingle | Xinfu LIN Ling SHI Hengzhao ZHU Sujuan CHEN Xuehua ZHENG Yiman WU Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells 康复学报 Stroke-Ⅱoral liquid PC12 cell TLR4/MyD88/NF-κB signal pathway inflammatory cascade |
| title | Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells |
| title_full | Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells |
| title_fullStr | Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells |
| title_full_unstemmed | Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells |
| title_short | Effects of Stroke-ⅡOral Liquid on TLR4/MyD88/NF-κB Signal Pathway in Chemical Damaged PC12 Cells |
| title_sort | effects of stroke iioral liquid on tlr4 myd88 nf κb signal pathway in chemical damaged pc12 cells |
| topic | Stroke-Ⅱoral liquid PC12 cell TLR4/MyD88/NF-κB signal pathway inflammatory cascade |
| url | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2016.06028 |
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