The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants
The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeti...
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MDPI AG
2024-12-01
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| Series: | Agriculture |
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| author | Wanqing Tan Zhiyuan Wang Liezhao Liu |
| author_facet | Wanqing Tan Zhiyuan Wang Liezhao Liu |
| author_sort | Wanqing Tan |
| collection | DOAJ |
| description | The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeting locations, and the optimization of CRISPR vectors. In this review, we systematically describe the Class II Cas proteins that have been used in plants, deactivated Cas9 (dCas9) and its role in transcriptional regulation, precision editing systems, Cas9 protein variants, as well as methods and examples of CRISPR–Cas systems targeting various regions with different breadths. In addition, we outline the optimization plans based on CRISPR constructs that can overcome the pleiotropy of genes or accelerate the generation of transgene-free plants and the applications of CRISPR systems in plant breeding practices. Finally, we discuss the theory and development of “CRISPR plus”, and the integrated application of existing systems in more species. |
| format | Article |
| id | doaj-art-90814bed0af5461098366a726bd26e93 |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-90814bed0af5461098366a726bd26e932025-01-10T13:13:27ZengMDPI AGAgriculture2077-04722024-12-011512910.3390/agriculture15010029The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in PlantsWanqing Tan0Zhiyuan Wang1Liezhao Liu2Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaThe creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this system has been continuously improved to meet human needs through the expansion and modification of Cas proteins, the diversification of targeting locations, and the optimization of CRISPR vectors. In this review, we systematically describe the Class II Cas proteins that have been used in plants, deactivated Cas9 (dCas9) and its role in transcriptional regulation, precision editing systems, Cas9 protein variants, as well as methods and examples of CRISPR–Cas systems targeting various regions with different breadths. In addition, we outline the optimization plans based on CRISPR constructs that can overcome the pleiotropy of genes or accelerate the generation of transgene-free plants and the applications of CRISPR systems in plant breeding practices. Finally, we discuss the theory and development of “CRISPR plus”, and the integrated application of existing systems in more species.https://www.mdpi.com/2077-0472/15/1/29CRISPR–Casprecise editingCRISPR screentransgene-freecrop improvement |
| spellingShingle | Wanqing Tan Zhiyuan Wang Liezhao Liu The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants Agriculture CRISPR–Cas precise editing CRISPR screen transgene-free crop improvement |
| title | The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants |
| title_full | The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants |
| title_fullStr | The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants |
| title_full_unstemmed | The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants |
| title_short | The Continuous Improvement of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-Associated Protein System Has Led to Its Highly Efficient Application in Plants |
| title_sort | continuous improvement of the clustered regularly interspaced short palindromic repeats crispr crispr associated protein system has led to its highly efficient application in plants |
| topic | CRISPR–Cas precise editing CRISPR screen transgene-free crop improvement |
| url | https://www.mdpi.com/2077-0472/15/1/29 |
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