Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples
Background: Vernonia amygdalina (Del.) has been reported to have medicinal values, and the phytochemical constituents which is present in different parts of the plant have been found to be responsible for the antimicrobial efficacy of the plant. This study focuses on the antibacterial activity of th...
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Zagazig University, Faculty of Medicine
2024-08-01
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| Series: | Microbes and Infectious Diseases |
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| Online Access: | https://mid.journals.ekb.eg/article_307481_484b11931cbe31624bff02fbd92b8433.pdf |
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| author | Anthony Onifade Damilola Akinyemi Ayodele Ogundare |
| author_facet | Anthony Onifade Damilola Akinyemi Ayodele Ogundare |
| author_sort | Anthony Onifade |
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| description | Background: Vernonia amygdalina (Del.) has been reported to have medicinal values, and the phytochemical constituents which is present in different parts of the plant have been found to be responsible for the antimicrobial efficacy of the plant. This study focuses on the antibacterial activity of the stem bark extracts of V. amygdalina against Multiple Antibiotic-Resistant (MAR) bacteria isolated from wound samples and also characterize the bioactive compounds present in the plant. Methods: Bacteria were isolated from wound samples using standard microbiological techniques and disc diffusion method was used to determine the sensitivity patterns of the isolated bacteria to conventional antibiotics and to the various extracts of V. amygdalina stem bark. Ciprofloxacin was used as control during the antibacterial assay. GC-MS analysis was carried out to identify the bioactive compounds in the stem bark extracts of the plant. Results: Pseudomonas aeruginosa (36%), Staphylococcus aureus (29%), Staphylococcus epidermidis (14%), Bacillus subtilis (6%), Escherichia coli (6%), Klebsiella pneumoniae (5%), and Proteus mirabilis (4%) were the bacteria isolated from the wound swabs sampled in this study. The ethyl acetate extract of V. amygdalina (300mg/ml) inhibited the growth of these organisms with the greatest effect on S. aureus with inhibition zone of 23.17±0.60 mm. The GC-MS analysis of the plant extracts revealed the presence of bioactive compounds such as coumarin, oleic acid, among others. Conclusion: The findings from this study have further established that V. amygdalina is a promising candidate and effective alternative treatment means for MAR bacteria that are associated with wound infections. |
| format | Article |
| id | doaj-art-8ff72b9258eb4f34a3f0e9d87f52ba4c |
| institution | Kabale University |
| issn | 2682-4132 2682-4140 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | Zagazig University, Faculty of Medicine |
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| series | Microbes and Infectious Diseases |
| spelling | doaj-art-8ff72b9258eb4f34a3f0e9d87f52ba4c2024-12-21T21:03:41ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402024-08-01531228123910.21608/mid.2023.219473.1550307481Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samplesAnthony Onifade0Damilola Akinyemi1Ayodele Ogundare2Department of Microbiology, School of Life Sciences, The Federal University of Technology, Akure, Ondo State, NigeriaDepartment of Microbiology, School of Life Sciences, The Federal University of Technology, Akure, Ondo State, NigeriaDepartment of Microbiology, School of Life Sciences, The Federal University of Technology, Akure, Ondo State, NigeriaBackground: Vernonia amygdalina (Del.) has been reported to have medicinal values, and the phytochemical constituents which is present in different parts of the plant have been found to be responsible for the antimicrobial efficacy of the plant. This study focuses on the antibacterial activity of the stem bark extracts of V. amygdalina against Multiple Antibiotic-Resistant (MAR) bacteria isolated from wound samples and also characterize the bioactive compounds present in the plant. Methods: Bacteria were isolated from wound samples using standard microbiological techniques and disc diffusion method was used to determine the sensitivity patterns of the isolated bacteria to conventional antibiotics and to the various extracts of V. amygdalina stem bark. Ciprofloxacin was used as control during the antibacterial assay. GC-MS analysis was carried out to identify the bioactive compounds in the stem bark extracts of the plant. Results: Pseudomonas aeruginosa (36%), Staphylococcus aureus (29%), Staphylococcus epidermidis (14%), Bacillus subtilis (6%), Escherichia coli (6%), Klebsiella pneumoniae (5%), and Proteus mirabilis (4%) were the bacteria isolated from the wound swabs sampled in this study. The ethyl acetate extract of V. amygdalina (300mg/ml) inhibited the growth of these organisms with the greatest effect on S. aureus with inhibition zone of 23.17±0.60 mm. The GC-MS analysis of the plant extracts revealed the presence of bioactive compounds such as coumarin, oleic acid, among others. Conclusion: The findings from this study have further established that V. amygdalina is a promising candidate and effective alternative treatment means for MAR bacteria that are associated with wound infections.https://mid.journals.ekb.eg/article_307481_484b11931cbe31624bff02fbd92b8433.pdfwoundantimicrobial resistancebacteriavernonia amygdalinainhibition zone |
| spellingShingle | Anthony Onifade Damilola Akinyemi Ayodele Ogundare Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples Microbes and Infectious Diseases wound antimicrobial resistance bacteria vernonia amygdalina inhibition zone |
| title | Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples |
| title_full | Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples |
| title_fullStr | Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples |
| title_full_unstemmed | Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples |
| title_short | Antibacterial activities of Vernonia amygdalina (Del.) stem bark extracts on multiple antibiotic-resistant bacteria isolated from wound samples |
| title_sort | antibacterial activities of vernonia amygdalina del stem bark extracts on multiple antibiotic resistant bacteria isolated from wound samples |
| topic | wound antimicrobial resistance bacteria vernonia amygdalina inhibition zone |
| url | https://mid.journals.ekb.eg/article_307481_484b11931cbe31624bff02fbd92b8433.pdf |
| work_keys_str_mv | AT anthonyonifade antibacterialactivitiesofvernoniaamygdalinadelstembarkextractsonmultipleantibioticresistantbacteriaisolatedfromwoundsamples AT damilolaakinyemi antibacterialactivitiesofvernoniaamygdalinadelstembarkextractsonmultipleantibioticresistantbacteriaisolatedfromwoundsamples AT ayodeleogundare antibacterialactivitiesofvernoniaamygdalinadelstembarkextractsonmultipleantibioticresistantbacteriaisolatedfromwoundsamples |