Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum
ObjectiveThis study aimed to screen the differentially expressed genes (DEGs) of Gossypium hirsutum under UV-B stress and identify the significant pathways based on gene enrichment analysis results.MethodsIn this study, the allotetraploid crop G. hirsutum was used to examine changes in various physi...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1529912/full |
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| author | Xiaolin Song Yingjie Zhu Ying Bao |
| author_facet | Xiaolin Song Yingjie Zhu Ying Bao |
| author_sort | Xiaolin Song |
| collection | DOAJ |
| description | ObjectiveThis study aimed to screen the differentially expressed genes (DEGs) of Gossypium hirsutum under UV-B stress and identify the significant pathways based on gene enrichment analysis results.MethodsIn this study, the allotetraploid crop G. hirsutum was used to examine changes in various physiological indexes under UV-B stress, and screened out all DEGs under UV-B stress (16 kJ m-2 d-1) based on six leaf transcriptomes. The main enrichment pathways of DEGs were analyzed according to gene annotation. Finally, we predicted the regulatory genes of phenylpropanoid pathway under UV-B stress by co-expression network analysis, and selected GhMYB4 for verification.ResultsGene annotation analysis revealed that DEGs were predominantly enriched in pathways related to photosynthesis and secondary metabolism. Further analysis revealed that UV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas G. hirsutum responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin. We selected the regulatory genes GhMYB4 for verification. It was found to be an anthocyanin negative regulator in response to UV-B stress.ConclusionsUV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas G. hirsutum responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin. |
| format | Article |
| id | doaj-art-8e789038de6445c1b4263c1712e4a3e6 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-8e789038de6445c1b4263c1712e4a3e62025-01-15T06:10:45ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15299121529912Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutumXiaolin SongYingjie ZhuYing BaoObjectiveThis study aimed to screen the differentially expressed genes (DEGs) of Gossypium hirsutum under UV-B stress and identify the significant pathways based on gene enrichment analysis results.MethodsIn this study, the allotetraploid crop G. hirsutum was used to examine changes in various physiological indexes under UV-B stress, and screened out all DEGs under UV-B stress (16 kJ m-2 d-1) based on six leaf transcriptomes. The main enrichment pathways of DEGs were analyzed according to gene annotation. Finally, we predicted the regulatory genes of phenylpropanoid pathway under UV-B stress by co-expression network analysis, and selected GhMYB4 for verification.ResultsGene annotation analysis revealed that DEGs were predominantly enriched in pathways related to photosynthesis and secondary metabolism. Further analysis revealed that UV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas G. hirsutum responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin. We selected the regulatory genes GhMYB4 for verification. It was found to be an anthocyanin negative regulator in response to UV-B stress.ConclusionsUV-B stress impaired photosynthesis mainly by damaging photosystem II (PSII) and inhibiting electron transport, whereas G. hirsutum responded to UV-B stress by synthesizing secondary metabolites such as anthocyanins and lignin.https://www.frontiersin.org/articles/10.3389/fpls.2024.1529912/fullphotosynthesisUV-B stressphenylpropanoid metabolismGossypium hirsutumco-expression networkanthocyanin biosynthesis |
| spellingShingle | Xiaolin Song Yingjie Zhu Ying Bao Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum Frontiers in Plant Science photosynthesis UV-B stress phenylpropanoid metabolism Gossypium hirsutum co-expression network anthocyanin biosynthesis |
| title | Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum |
| title_full | Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum |
| title_fullStr | Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum |
| title_full_unstemmed | Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum |
| title_short | Identification and characteristics of differentially expressed genes under UV-B stress in Gossypium hirsutum |
| title_sort | identification and characteristics of differentially expressed genes under uv b stress in gossypium hirsutum |
| topic | photosynthesis UV-B stress phenylpropanoid metabolism Gossypium hirsutum co-expression network anthocyanin biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1529912/full |
| work_keys_str_mv | AT xiaolinsong identificationandcharacteristicsofdifferentiallyexpressedgenesunderuvbstressingossypiumhirsutum AT yingjiezhu identificationandcharacteristicsofdifferentiallyexpressedgenesunderuvbstressingossypiumhirsutum AT yingbao identificationandcharacteristicsofdifferentiallyexpressedgenesunderuvbstressingossypiumhirsutum |