Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA)
The aim of the study was to research the microflora of suppurative complications, its antibiotic resistance, and the molecular genetic features of MRSA in burn patients. Material and methods. The microflora of biopsy specimens, wound fluid from 145 patients with thermal burns was investigated on the...
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| Format: | Article |
| Language: | Russian |
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LLC "Publishing House OKI"
2020-05-01
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| Series: | Антибиотики и Химиотерапия |
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| Online Access: | https://www.antibiotics-chemotherapy.ru/jour/article/view/49 |
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| author | O. E. Khokhlova O. V. Peryanova I. V. Vladimirov V. A. Matskevich N. K. Potkina D. N. Kapshuk L. N. Kopytko V. V. Gostev S. V. Sidorenko Ya. .. Iwao T. .. Yamamoto |
| author_facet | O. E. Khokhlova O. V. Peryanova I. V. Vladimirov V. A. Matskevich N. K. Potkina D. N. Kapshuk L. N. Kopytko V. V. Gostev S. V. Sidorenko Ya. .. Iwao T. .. Yamamoto |
| author_sort | O. E. Khokhlova |
| collection | DOAJ |
| description | The aim of the study was to research the microflora of suppurative complications, its antibiotic resistance, and the molecular genetic features of MRSA in burn patients. Material and methods. The microflora of biopsy specimens, wound fluid from 145 patients with thermal burns was investigated on the first day and on the 10-50th days of stay in the burn center of the Regional Clinical Hospital in 2013-2016. The molecular genetic features of 12 strains of MRSA isolated from different burn patients have been studied. Methods used: bacteriological, PCR, M-PCR, sequencing, PFGE, agglutination reaction. Results. On the first day of hospitalization, when biopsy specimen and wound fluid were taken, microbial growth was detected in 25.5% of cases, gram-positive microorganisms, in particular MSSA (37.8%), dominated. On the 10-50th days of hospitalization, growth was obtained in 93.2% of cases, Gram-negative microflora (53.4%) dominated, with a significant role played by Pseudomonas spp. and Acinetobacter spp. (38.0%), characterized by a high degree of resistance to antimicrobial drugs: XDR - 18%, PDR - 67%. On the 10-50th days of hospitalization, a significant role was played by staphylococci (38.7%), while the proportion of MRSA was 62.2%. Circulation of one MRSA clone in burn patients - ST239/spa3(t037)/a§r//SCCmecIII.1.1.2(IIIA)/CoaIy, characterized by multiple resistance to antimicrobial agents, was established. |
| format | Article |
| id | doaj-art-8c6049ea555841cd9c507e22c69d8dc8 |
| institution | Kabale University |
| issn | 0235-2990 |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | LLC "Publishing House OKI" |
| record_format | Article |
| series | Антибиотики и Химиотерапия |
| spelling | doaj-art-8c6049ea555841cd9c507e22c69d8dc82025-08-20T03:59:08ZrusLLC "Publishing House OKI"Антибиотики и Химиотерапия0235-29902020-05-01629-10273348Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA)O. E. Khokhlova0O. V. Peryanova1I. V. Vladimirov2V. A. Matskevich3N. K. Potkina4D. N. Kapshuk5L. N. Kopytko6V. V. Gostev7S. V. Sidorenko8Ya. .. Iwao9T. .. Yamamoto10Krasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetski; Russia-Japan Center of Microbiology, Metagenomics and Infectious DiseasesKrasnoyarsk State Medical University named after Prof. V.F.Voino-Yasenetski; Russia-Japan Center of Microbiology, Metagenomics and Infectious DiseasesRegional Clinical HospitalRegional Clinical HospitalRussia-Japan Center of Microbiology, Metagenomics and Infectious DiseasesKrasnoyarsk State Medical University named after Prof. V.F.Voino-YasenetskiRegional Clinical HospitalChildren's Scientific and Clinical Center for Infectious Diseases of the Federal Medical Biological AgencyChildren's Scientific and Clinical Center for Infectious Diseases of the Federal Medical Biological AgencyInternational Medical Educational Research Center (IMERC)Russia-Japan Center of Microbiology, Metagenomics and Infectious Diseases; International Medical Educational Research Center (IMERC)The aim of the study was to research the microflora of suppurative complications, its antibiotic resistance, and the molecular genetic features of MRSA in burn patients. Material and methods. The microflora of biopsy specimens, wound fluid from 145 patients with thermal burns was investigated on the first day and on the 10-50th days of stay in the burn center of the Regional Clinical Hospital in 2013-2016. The molecular genetic features of 12 strains of MRSA isolated from different burn patients have been studied. Methods used: bacteriological, PCR, M-PCR, sequencing, PFGE, agglutination reaction. Results. On the first day of hospitalization, when biopsy specimen and wound fluid were taken, microbial growth was detected in 25.5% of cases, gram-positive microorganisms, in particular MSSA (37.8%), dominated. On the 10-50th days of hospitalization, growth was obtained in 93.2% of cases, Gram-negative microflora (53.4%) dominated, with a significant role played by Pseudomonas spp. and Acinetobacter spp. (38.0%), characterized by a high degree of resistance to antimicrobial drugs: XDR - 18%, PDR - 67%. On the 10-50th days of hospitalization, a significant role was played by staphylococci (38.7%), while the proportion of MRSA was 62.2%. Circulation of one MRSA clone in burn patients - ST239/spa3(t037)/a§r//SCCmecIII.1.1.2(IIIA)/CoaIy, characterized by multiple resistance to antimicrobial agents, was established.https://www.antibiotics-chemotherapy.ru/jour/article/view/49burn patientsmicroflora of purulent complicationsantibiotic resistancemethicillin-resistant staphylococcus aureusgenotyping |
| spellingShingle | O. E. Khokhlova O. V. Peryanova I. V. Vladimirov V. A. Matskevich N. K. Potkina D. N. Kapshuk L. N. Kopytko V. V. Gostev S. V. Sidorenko Ya. .. Iwao T. .. Yamamoto Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) Антибиотики и Химиотерапия burn patients microflora of purulent complications antibiotic resistance methicillin-resistant staphylococcus aureus genotyping |
| title | Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) |
| title_full | Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) |
| title_fullStr | Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) |
| title_full_unstemmed | Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) |
| title_short | Microbiological Monitoring of Purulent Complications in Burn Patients and Molecular Genetic Features of Methicillin-Resistant Staphylococcus aureus (MRSA) |
| title_sort | microbiological monitoring of purulent complications in burn patients and molecular genetic features of methicillin resistant staphylococcus aureus mrsa |
| topic | burn patients microflora of purulent complications antibiotic resistance methicillin-resistant staphylococcus aureus genotyping |
| url | https://www.antibiotics-chemotherapy.ru/jour/article/view/49 |
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