Single cell variability of CRISPR‐Cas interference and adaptation

Single cell variability of CRISPR‐Cas interference and adaptation

Abstract While CRISPR‐Cas defence mechanisms have been studied on a population level, their temporal dynamics and variability in individual cells have remained unknown. Using a microfluidic device, time‐lapse microscopy and mathematical modelling, we studied invader clearance in Escherichia coli acr...

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Bibliographic Details
Main Authors: Rebecca E McKenzie, Emma M Keizer, Jochem N A Vink, Jasper van Lopik, Ferhat Büke, Vera Kalkman, Christian Fleck, Sander J Tans, Stan J J Brouns
Format: Article
Language:English
Published: Springer Nature 2022-04-01
Series:Molecular Systems Biology
Subjects:
agent‐based simulations
single‐cell analysis
spacer acquisition
time‐lapse microscopy
type I CRISPR‐Cas
Online Access:https://doi.org/10.15252/msb.202110680
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