Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation
Background Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. Previous research has confirmed that isofraxidin can reduce macrophage expression and inhibit peripheral inflammation. However, its effects on the central nervous system remain underexplored. Objective This st...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-01-01
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| Series: | Journal of Central Nervous System Disease |
| Online Access: | https://doi.org/10.1177/11795735241312661 |
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| author | Tin-An Wang Shiao-Yun Li Li-Yun Fann I-Hsun Li Tsung-Ta Liu Hao-Yuan Hung Chieh-Wen Chang Chih-Chien Cheng Ying-Che Huang Pei-Yeh Yu Jui-Hu Shih |
| author_facet | Tin-An Wang Shiao-Yun Li Li-Yun Fann I-Hsun Li Tsung-Ta Liu Hao-Yuan Hung Chieh-Wen Chang Chih-Chien Cheng Ying-Che Huang Pei-Yeh Yu Jui-Hu Shih |
| author_sort | Tin-An Wang |
| collection | DOAJ |
| description | Background Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. Previous research has confirmed that isofraxidin can reduce macrophage expression and inhibit peripheral inflammation. However, its effects on the central nervous system remain underexplored. Objective This study aims to determine whether isofraxidin offers protective effects against PD. Methods To assess the effects of isofraxidin, motor performance changes in LPS-induced PD mice were evaluated using rotarod, pole-climbing, and beam-walking tests. Striatal damage was examined through [ 18 F]fluorodeoxyglucose ([ 18 F]FDG) positron emission tomography (PET) imaging, and dopaminergic neurotoxicity was assessed using tyrosine hydroxylase (TH) staining. Microglial accumulation and activation were monitored with Iba-1 staining, while LPS-induced inflammation was examined via TNF-α and IL-1β staining. Results Isofraxidin pre-treatment significantly improved LPS-induced motor dysfunction, as evidenced by better performance in the rotarod, pole-climbing, and beam-walking tests. [ 18 F]FDG PET imaging showed that isofraxidin restored glucose uptake in the striatum, countering LPS-induced damage. Furthermore, Iba-1 staining revealed that isofraxidin markedly inhibited LPS-induced microglial activation and accumulation. TNF-α and IL-1β staining indicated a reduction in inflammation with isofraxidin treatment. Additionally, TH staining supported the neuroprotective role of isofraxidin on dopaminergic neurons. Conclusions Isofraxidin exhibits notable neuroprotective properties by mitigating LPS-induced parkinsonian behaviors, microglial activation, inflammation, and dopaminergic neuron damage. These results highlight isofraxidin’s potential as a therapeutic intervention for PD. |
| format | Article |
| id | doaj-art-89bf6e5b92ad419997b3b9b4bc2df632 |
| institution | Kabale University |
| issn | 1179-5735 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Central Nervous System Disease |
| spelling | doaj-art-89bf6e5b92ad419997b3b9b4bc2df6322025-01-09T13:03:19ZengSAGE PublishingJournal of Central Nervous System Disease1179-57352025-01-011710.1177/11795735241312661Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activationTin-An WangShiao-Yun LiLi-Yun FannI-Hsun LiTsung-Ta LiuHao-Yuan HungChieh-Wen ChangChih-Chien ChengYing-Che HuangPei-Yeh YuJui-Hu ShihBackground Parkinson’s disease (PD) is one of the most common neurodegenerative disorders. Previous research has confirmed that isofraxidin can reduce macrophage expression and inhibit peripheral inflammation. However, its effects on the central nervous system remain underexplored. Objective This study aims to determine whether isofraxidin offers protective effects against PD. Methods To assess the effects of isofraxidin, motor performance changes in LPS-induced PD mice were evaluated using rotarod, pole-climbing, and beam-walking tests. Striatal damage was examined through [ 18 F]fluorodeoxyglucose ([ 18 F]FDG) positron emission tomography (PET) imaging, and dopaminergic neurotoxicity was assessed using tyrosine hydroxylase (TH) staining. Microglial accumulation and activation were monitored with Iba-1 staining, while LPS-induced inflammation was examined via TNF-α and IL-1β staining. Results Isofraxidin pre-treatment significantly improved LPS-induced motor dysfunction, as evidenced by better performance in the rotarod, pole-climbing, and beam-walking tests. [ 18 F]FDG PET imaging showed that isofraxidin restored glucose uptake in the striatum, countering LPS-induced damage. Furthermore, Iba-1 staining revealed that isofraxidin markedly inhibited LPS-induced microglial activation and accumulation. TNF-α and IL-1β staining indicated a reduction in inflammation with isofraxidin treatment. Additionally, TH staining supported the neuroprotective role of isofraxidin on dopaminergic neurons. Conclusions Isofraxidin exhibits notable neuroprotective properties by mitigating LPS-induced parkinsonian behaviors, microglial activation, inflammation, and dopaminergic neuron damage. These results highlight isofraxidin’s potential as a therapeutic intervention for PD.https://doi.org/10.1177/11795735241312661 |
| spellingShingle | Tin-An Wang Shiao-Yun Li Li-Yun Fann I-Hsun Li Tsung-Ta Liu Hao-Yuan Hung Chieh-Wen Chang Chih-Chien Cheng Ying-Che Huang Pei-Yeh Yu Jui-Hu Shih Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation Journal of Central Nervous System Disease |
| title | Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation |
| title_full | Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation |
| title_fullStr | Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation |
| title_full_unstemmed | Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation |
| title_short | Neuroprotective potential of isofraxidin: Alleviating parkinsonian symptoms, inflammation and microglial activation |
| title_sort | neuroprotective potential of isofraxidin alleviating parkinsonian symptoms inflammation and microglial activation |
| url | https://doi.org/10.1177/11795735241312661 |
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