Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens
The utilization of chemical pesticides recovers 30%−40% of food losses. However, their application has also triggered a series of problems, including food safety, environmental pollution, pesticide resistance, and incidents of poisoning. Consequently, green pesticides are increasingly seen as viable...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498653/full |
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| author | Yanli Zheng Tongshu Liu Ziyu Wang Xu Wang Haiyan Wang Ying Li Wangshan Zheng Shiyu Wei Yan Leng Jiajia Li Yan Yang Yang Liu Zhaoyu Li Qiang Wang Qiang Wang Yongqiang Tian |
| author_facet | Yanli Zheng Tongshu Liu Ziyu Wang Xu Wang Haiyan Wang Ying Li Wangshan Zheng Shiyu Wei Yan Leng Jiajia Li Yan Yang Yang Liu Zhaoyu Li Qiang Wang Qiang Wang Yongqiang Tian |
| author_sort | Yanli Zheng |
| collection | DOAJ |
| description | The utilization of chemical pesticides recovers 30%−40% of food losses. However, their application has also triggered a series of problems, including food safety, environmental pollution, pesticide resistance, and incidents of poisoning. Consequently, green pesticides are increasingly seen as viable alternatives to their chemical counterparts. Among these, Plant Growth-Promoting Rhizobacteria (PGPR), which are found within plant rhizosphere, stand out for their capacity to stimulate plant growth. Recently, we isolated a strain, BN, with broad-spectrum antimicrobial activity from the rhizosphere of Lilium brownii. Identification revealed that this strain belongs to the species Bacillus velezensis and exhibits significant inhibitory effects against various fungal plant pathogens. The complete genome sequence of B. velezensis BN consists of a circular chromosome with a length of 3,929,791 bp, includes 3,747 protein-coding genes, 81 small RNAs, 27 rRNAs, and 86 tRNAs. Genomic analysis revealed that 29% of the genes are directly involved in plant growth, while 70% of the genes are indirectly involved. In addition, 12 putative biosynthetic gene clusters were identified, responsible for the synthesis of secondary metabolites, such as non-ribosomal peptides, lanthipeptides, polyketides, siderophores, and terpenes. These findings provide a scientific basis for the development of efficient antimicrobial agents and the construction of biopesticide production platforms in chassis cells. |
| format | Article |
| id | doaj-art-87b209d2b498461fa378d5e9699241df |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-87b209d2b498461fa378d5e9699241df2025-01-03T05:10:25ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.14986531498653Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogensYanli Zheng0Tongshu Liu1Ziyu Wang2Xu Wang3Haiyan Wang4Ying Li5Wangshan Zheng6Shiyu Wei7Yan Leng8Jiajia Li9Yan Yang10Yang Liu11Zhaoyu Li12Qiang Wang13Qiang Wang14Yongqiang Tian15School of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaState Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, ChinaAcademy for Advanced Interdisciplinary Studies, Henan University, Kaifeng, ChinaSchool of Biological and Pharmaceutical Engineering, Lanzhou Jiaotong University, Lanzhou, Gansu, ChinaThe utilization of chemical pesticides recovers 30%−40% of food losses. However, their application has also triggered a series of problems, including food safety, environmental pollution, pesticide resistance, and incidents of poisoning. Consequently, green pesticides are increasingly seen as viable alternatives to their chemical counterparts. Among these, Plant Growth-Promoting Rhizobacteria (PGPR), which are found within plant rhizosphere, stand out for their capacity to stimulate plant growth. Recently, we isolated a strain, BN, with broad-spectrum antimicrobial activity from the rhizosphere of Lilium brownii. Identification revealed that this strain belongs to the species Bacillus velezensis and exhibits significant inhibitory effects against various fungal plant pathogens. The complete genome sequence of B. velezensis BN consists of a circular chromosome with a length of 3,929,791 bp, includes 3,747 protein-coding genes, 81 small RNAs, 27 rRNAs, and 86 tRNAs. Genomic analysis revealed that 29% of the genes are directly involved in plant growth, while 70% of the genes are indirectly involved. In addition, 12 putative biosynthetic gene clusters were identified, responsible for the synthesis of secondary metabolites, such as non-ribosomal peptides, lanthipeptides, polyketides, siderophores, and terpenes. These findings provide a scientific basis for the development of efficient antimicrobial agents and the construction of biopesticide production platforms in chassis cells.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498653/fullgreen pesticidesfood safetyPlant Growth-Promoting Rhizobacteria (PGPR)antimicrobial agentsnon-ribosomal peptides |
| spellingShingle | Yanli Zheng Tongshu Liu Ziyu Wang Xu Wang Haiyan Wang Ying Li Wangshan Zheng Shiyu Wei Yan Leng Jiajia Li Yan Yang Yang Liu Zhaoyu Li Qiang Wang Qiang Wang Yongqiang Tian Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens Frontiers in Microbiology green pesticides food safety Plant Growth-Promoting Rhizobacteria (PGPR) antimicrobial agents non-ribosomal peptides |
| title | Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens |
| title_full | Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens |
| title_fullStr | Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens |
| title_full_unstemmed | Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens |
| title_short | Whole-genome sequencing and secondary metabolite exploration of the novel Bacillus velezensis BN with broad-spectrum antagonistic activity against fungal plant pathogens |
| title_sort | whole genome sequencing and secondary metabolite exploration of the novel bacillus velezensis bn with broad spectrum antagonistic activity against fungal plant pathogens |
| topic | green pesticides food safety Plant Growth-Promoting Rhizobacteria (PGPR) antimicrobial agents non-ribosomal peptides |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1498653/full |
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