Sperm cellular and nuclear dynamics associated with ram fertility
The aim of this study is to analyze DNA integrity, shape morphology, and membrane integrity in sperm from low-fertility (LF) and adequate- or normal-fertility (AF) rams. Various sperm evaluation methods such as sperm chromatin dispersion assay, Fourier harmonic amplitude (FHA) analysis, and other im...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1577004/full |
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| author | Mustafa Bodu Mustafa Bodu Mustafa Hitit Mustafa Hitit Ayse Sari Mesut Kirbas Bulent Bulbul Mehmet Bozkurt Ataman Mustafa Numan Bucak John Parrish Abdullah Kaya Abdullah Kaya Erdogan Memili |
| author_facet | Mustafa Bodu Mustafa Bodu Mustafa Hitit Mustafa Hitit Ayse Sari Mesut Kirbas Bulent Bulbul Mehmet Bozkurt Ataman Mustafa Numan Bucak John Parrish Abdullah Kaya Abdullah Kaya Erdogan Memili |
| author_sort | Mustafa Bodu |
| collection | DOAJ |
| description | The aim of this study is to analyze DNA integrity, shape morphology, and membrane integrity in sperm from low-fertility (LF) and adequate- or normal-fertility (AF) rams. Various sperm evaluation methods such as sperm chromatin dispersion assay, Fourier harmonic amplitude (FHA) analysis, and other image analysis of morphometric parameters were used. An additional aim is to employ new statistical models with high reliability to predict ram fertility based on sperm head morphology parameters. Fresh semen was collected from 41 AF (conception rate 95.1% ± 0.6%) and 27 LF (conception rate 79.7% ± 2.5%) rams using artificial vagina. Differences (p < 0.05) were observed in percent motile sperm (mean ± SEM, 64% ± 3%, 72% ± 2%), percent viable sperm (78 ± 2%, 84 ± 1%), and head and acrosome abnormalities (1.9% ± 0.4%, 3.4% ± 0.4%) between LF and AF rams. The findings of different analyses also showed that the fertility of rams is not associated with DNA fragmentation (p > 0.05). Using the FHA analysis, an average head shape of ram sperm was constructed and harmonic amplitude 2 was determined, which tended to differ between the two ram fertility groups (p = 0.059). Based on the FHA and morphometric analysis, a significant linear discriminant model was constructed (p = 0.0013), which allowed for specificity in identifying LF rams (6/9, 66.7%) and sensitivity in identifying AF rams (39/47, 83.0%). The overall error rate remained good, which was 11/56 (20%). The findings of this study suggest that sperm DNA damage might not be used to predict ram fertility and that the statistical model based on the FHA analysis can be a potential tool in predicting ram fertility. |
| format | Article |
| id | doaj-art-8524c3f44b4a4ac2b38c8d92b13dd2e3 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-8524c3f44b4a4ac2b38c8d92b13dd2e32025-08-20T03:47:41ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-05-011210.3389/fvets.2025.15770041577004Sperm cellular and nuclear dynamics associated with ram fertilityMustafa Bodu0Mustafa Bodu1Mustafa Hitit2Mustafa Hitit3Ayse Sari4Mesut Kirbas5Bulent Bulbul6Mehmet Bozkurt Ataman7Mustafa Numan Bucak8John Parrish9Abdullah Kaya10Abdullah Kaya11Erdogan Memili12Cooperative Agricultural Research Center, College of Agriculture, Food and Natural Resources, Prairie View A&M University, Prairie View, TX, United StatesDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, TürkiyeCooperative Agricultural Research Center, College of Agriculture, Food and Natural Resources, Prairie View A&M University, Prairie View, TX, United StatesDepartment of Genetics, Faculty of Veterinary Medicine, Kastamonu University, Kastamonu, TürkiyeDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Necmettin Erbakan University, Konya, TürkiyeBahri Dagdas International Agricultural Research Institute, Konya, TürkiyeDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Dokuz Eylul University, Izmir, TürkiyeDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, TürkiyeDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, TürkiyeDepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI, United StatesDepartment of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, TürkiyeDepartment of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI, United StatesCooperative Agricultural Research Center, College of Agriculture, Food and Natural Resources, Prairie View A&M University, Prairie View, TX, United StatesThe aim of this study is to analyze DNA integrity, shape morphology, and membrane integrity in sperm from low-fertility (LF) and adequate- or normal-fertility (AF) rams. Various sperm evaluation methods such as sperm chromatin dispersion assay, Fourier harmonic amplitude (FHA) analysis, and other image analysis of morphometric parameters were used. An additional aim is to employ new statistical models with high reliability to predict ram fertility based on sperm head morphology parameters. Fresh semen was collected from 41 AF (conception rate 95.1% ± 0.6%) and 27 LF (conception rate 79.7% ± 2.5%) rams using artificial vagina. Differences (p < 0.05) were observed in percent motile sperm (mean ± SEM, 64% ± 3%, 72% ± 2%), percent viable sperm (78 ± 2%, 84 ± 1%), and head and acrosome abnormalities (1.9% ± 0.4%, 3.4% ± 0.4%) between LF and AF rams. The findings of different analyses also showed that the fertility of rams is not associated with DNA fragmentation (p > 0.05). Using the FHA analysis, an average head shape of ram sperm was constructed and harmonic amplitude 2 was determined, which tended to differ between the two ram fertility groups (p = 0.059). Based on the FHA and morphometric analysis, a significant linear discriminant model was constructed (p = 0.0013), which allowed for specificity in identifying LF rams (6/9, 66.7%) and sensitivity in identifying AF rams (39/47, 83.0%). The overall error rate remained good, which was 11/56 (20%). The findings of this study suggest that sperm DNA damage might not be used to predict ram fertility and that the statistical model based on the FHA analysis can be a potential tool in predicting ram fertility.https://www.frontiersin.org/articles/10.3389/fvets.2025.1577004/fullramfertilityDNA integritysperm headFourier analysis |
| spellingShingle | Mustafa Bodu Mustafa Bodu Mustafa Hitit Mustafa Hitit Ayse Sari Mesut Kirbas Bulent Bulbul Mehmet Bozkurt Ataman Mustafa Numan Bucak John Parrish Abdullah Kaya Abdullah Kaya Erdogan Memili Sperm cellular and nuclear dynamics associated with ram fertility Frontiers in Veterinary Science ram fertility DNA integrity sperm head Fourier analysis |
| title | Sperm cellular and nuclear dynamics associated with ram fertility |
| title_full | Sperm cellular and nuclear dynamics associated with ram fertility |
| title_fullStr | Sperm cellular and nuclear dynamics associated with ram fertility |
| title_full_unstemmed | Sperm cellular and nuclear dynamics associated with ram fertility |
| title_short | Sperm cellular and nuclear dynamics associated with ram fertility |
| title_sort | sperm cellular and nuclear dynamics associated with ram fertility |
| topic | ram fertility DNA integrity sperm head Fourier analysis |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1577004/full |
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