Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells
The biogenic synthesis of metallic nanoparticles (MNPs) using plant extracts has been proposed as a facile and low-cost process that can enhance the antimicrobial and anticancer properties of those MNPs, suggesting that the plant extract capping can also improve biosafety. In this work, copper nanop...
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Elsevier
2025-06-01
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| Series: | Nano Trends |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666978125000510 |
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| author | Gilmer David Cab-Torres Lluvia López Daniela Salado-Leza Gabriela Navarro-Tovar |
| author_facet | Gilmer David Cab-Torres Lluvia López Daniela Salado-Leza Gabriela Navarro-Tovar |
| author_sort | Gilmer David Cab-Torres |
| collection | DOAJ |
| description | The biogenic synthesis of metallic nanoparticles (MNPs) using plant extracts has been proposed as a facile and low-cost process that can enhance the antimicrobial and anticancer properties of those MNPs, suggesting that the plant extract capping can also improve biosafety. In this work, copper nanoparticles (CuNPs) (86 ± 46 nm) were synthesized using a Crataegus rosei “tejocote” extract (rich in polyphenols and flavonoids), and the obtained particles were evaluated in human keratinocytes HaCaT cells (healthy cells) and tumor prostate PC-3 cells to determine toxicity and antitumor activity, respectively. The plant extract showed non or slight cytotoxic effects in both HaCaT and PC-3 cells at the tested concentrations. On the other hand, CuNPs synthesized with C. rosei extract showed an IC50 =120 ± 1.13 µg/mL in HaCaT cells, but in tumor PC-3 cells the IC50 = 491 ± 1.06 µg/mL. It is showed that C. rosei capping is insufficient to tune the oxidative stress in healthy cells exposed to CuNPs at higher concentrations, and, that higher concentrations of CuNPs are required to reduce the cell viability in <70 % in tumor PC-3 cells. Thus, a further study with different biogenic synthesized CuNPs particle size could determine different effects on the same models. It is also relevant to carry out in vitro analysis with other cancer cells and biomarkers. |
| format | Article |
| id | doaj-art-84caa6f734cc4c63af33d1b5cad1f55d |
| institution | Kabale University |
| issn | 2666-9781 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
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| series | Nano Trends |
| spelling | doaj-art-84caa6f734cc4c63af33d1b5cad1f55d2025-08-20T03:46:38ZengElsevierNano Trends2666-97812025-06-011010012210.1016/j.nwnano.2025.100122Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cellsGilmer David Cab-Torres0Lluvia López1Daniela Salado-Leza2Gabriela Navarro-Tovar3Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Manuel Nava 6, S.L.P., 78210, MexicoInstituto de Investigación de Zonas Desérticas, Universidad Autónoma de San Luis Potosí, Altaír 200, S.L.P., 78377, Mexico; Facultad de Ingeniería, Universidad Autónoma de San Luis Potosí, Manuel Nava 8, S.L.P., 78290, MexicoInstituto de Física, Universidad Autónoma de San Luis Potosí, Parque Chapultepec 1570, S.L.P., 78295, Mexico; Consejo Nacional Humanidades, Ciencia y Tecnología, Insurgentes Sur 1582, Mexico City, 03940, Mexico; Corresponding authors at: Universidad Autónoma de San Luis Potosí, and Consejo Nacional de Humanidades, Ciencia y Tecnología, Insurgentes Sur 1582, Mexico City, 03940, Mexico.Facultad de Ciencias Químicas, Universidad Autónoma de San Luis Potosí, Manuel Nava 6, S.L.P., 78210, Mexico; Consejo Nacional Humanidades, Ciencia y Tecnología, Insurgentes Sur 1582, Mexico City, 03940, Mexico; Corresponding authors at: Universidad Autónoma de San Luis Potosí, and Consejo Nacional de Humanidades, Ciencia y Tecnología, Insurgentes Sur 1582, Mexico City, 03940, Mexico.The biogenic synthesis of metallic nanoparticles (MNPs) using plant extracts has been proposed as a facile and low-cost process that can enhance the antimicrobial and anticancer properties of those MNPs, suggesting that the plant extract capping can also improve biosafety. In this work, copper nanoparticles (CuNPs) (86 ± 46 nm) were synthesized using a Crataegus rosei “tejocote” extract (rich in polyphenols and flavonoids), and the obtained particles were evaluated in human keratinocytes HaCaT cells (healthy cells) and tumor prostate PC-3 cells to determine toxicity and antitumor activity, respectively. The plant extract showed non or slight cytotoxic effects in both HaCaT and PC-3 cells at the tested concentrations. On the other hand, CuNPs synthesized with C. rosei extract showed an IC50 =120 ± 1.13 µg/mL in HaCaT cells, but in tumor PC-3 cells the IC50 = 491 ± 1.06 µg/mL. It is showed that C. rosei capping is insufficient to tune the oxidative stress in healthy cells exposed to CuNPs at higher concentrations, and, that higher concentrations of CuNPs are required to reduce the cell viability in <70 % in tumor PC-3 cells. Thus, a further study with different biogenic synthesized CuNPs particle size could determine different effects on the same models. It is also relevant to carry out in vitro analysis with other cancer cells and biomarkers.http://www.sciencedirect.com/science/article/pii/S2666978125000510Copper nanoparticleCrataegus roseiBiogenic nanoparticle synthesisCytotoxicityHaCaT cells linePC-3 cells line |
| spellingShingle | Gilmer David Cab-Torres Lluvia López Daniela Salado-Leza Gabriela Navarro-Tovar Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells Nano Trends Copper nanoparticle Crataegus rosei Biogenic nanoparticle synthesis Cytotoxicity HaCaT cells line PC-3 cells line |
| title | Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| title_full | Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| title_fullStr | Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| title_full_unstemmed | Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| title_short | Harnessing Crataegus rosei “tejocote” for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| title_sort | harnessing crataegus rosei tejocote for biogenic synthesis of copper nanoparticles and cytotoxicity in normal and cancer cells |
| topic | Copper nanoparticle Crataegus rosei Biogenic nanoparticle synthesis Cytotoxicity HaCaT cells line PC-3 cells line |
| url | http://www.sciencedirect.com/science/article/pii/S2666978125000510 |
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