Engineering thioesterase as a driving force for novel itaconate production via its degradation scheme

Incorporation of irreversible steps in pathway design enhances the overall thermodynamic favorability and often leads to better bioconversion yield given functional enzymes. Using this concept, here we constructed the first non-natural itaconate biosynthesis pathway driven by thioester hydrolysis. I...

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Bibliographic Details
Main Authors: Ryan S. Wang, Siang-Wun Siao, Jessica C. Wang, Patrick Y. Lin, Claire R. Shen
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Metabolic Engineering Communications
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Online Access:http://www.sciencedirect.com/science/article/pii/S2214030124000154
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