Genomic and Phenotypic Characterization of CHO 4BGD Cells with Quad Knockout and Overexpression of Two Housekeeping Genes That Allow for Metabolic Selection and Extended Fed-Batch Culturing

Genomic and Phenotypic Characterization of CHO 4BGD Cells with Quad Knockout and Overexpression of Two Housekeeping Genes That Allow for Metabolic Selection and Extended Fed-Batch Culturing

Re-engineering of CHO cells using genome editing and the overexpression of multiple helper genes is the central track for obtaining better cell lines for the production of biopharmaceuticals. Using two subsequent rounds of genome editing of the CHO S cells, we have developed the cell line CHO 4BGD w...

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Bibliographic Details
Main Authors: Nadezhda Alexandrovna Orlova, Maria Valerievna Sinegubova, Denis Eduardovich Kolesov, Yulia Alexandrovna Khodak, Victor Vyacheslavovich Tatarskiy, Ivan Ivanovich Vorobiev
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Cells
Subjects:
CHO
bak1
bax
apoptosis
autophagy
genome editing
Online Access:https://www.mdpi.com/2073-4409/14/10/692
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