RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii
ABSTRACT RS4‐type resistant starches have been applied in food industry as thickeners or studied as functional foods for a long time. However, the influence and mechanism of RS4 on Type 2 diabetes mellitus (T2DM) have not been elucidated. In this study, sinapic acid starch ester (SASE) was chosen as...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Food Frontiers |
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| Online Access: | https://doi.org/10.1002/fft2.528 |
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| author | Tao Xu Yongheng Zhong Shengyang Ji Hang Xiao Jun Li Kaimian Li Peiwu Li Baiyi Lu |
| author_facet | Tao Xu Yongheng Zhong Shengyang Ji Hang Xiao Jun Li Kaimian Li Peiwu Li Baiyi Lu |
| author_sort | Tao Xu |
| collection | DOAJ |
| description | ABSTRACT RS4‐type resistant starches have been applied in food industry as thickeners or studied as functional foods for a long time. However, the influence and mechanism of RS4 on Type 2 diabetes mellitus (T2DM) have not been elucidated. In this study, sinapic acid starch ester (SASE) was chosen as a representative of functional RS4, and its anti‐diabetic activity and underlying mechanism were investigated. Results showed that SASE administration lowered blood glucose and ameliorated insulin resistance of streptozotocin‐induced diabetic mice via increasing serum butyrate level. Fecal microbiota transplantation confirmed the T2DM‐improving activity of SASE modulated gut microbiota. 16S rRNA gene sequencing indicated that SASE selectively promoted the proliferation of butyrate‐producing bacterium. In vitro co‐culture of promoted gut bacterium with SASE revealed that Lactobacillus johnsonii was one of the key bacteria in the degradation of SASE. In conclusion, RS4 could selectively promote gut bacteria and increase short‐chain fatty acid production to alleviate T2DM. |
| format | Article |
| id | doaj-art-8266b39f900d47f4a6c1c94e28c836e9 |
| institution | Kabale University |
| issn | 2643-8429 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Frontiers |
| spelling | doaj-art-8266b39f900d47f4a6c1c94e28c836e92025-08-20T03:47:40ZengWileyFood Frontiers2643-84292025-01-016126828110.1002/fft2.528RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsoniiTao Xu0Yongheng Zhong1Shengyang Ji2Hang Xiao3Jun Li4Kaimian Li5Peiwu Li6Baiyi Lu7College of Biosystems Engineering and Food Science, Key Laboratory for Agro‐Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs Zhejiang University Hangzhou ChinaCollege of Biosystems Engineering and Food Science, Key Laboratory for Agro‐Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs Zhejiang University Hangzhou ChinaCollege of Biosystems Engineering and Food Science, Key Laboratory for Agro‐Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs Zhejiang University Hangzhou ChinaDepartment of Food Science University of Massachusetts Amherst Amherst Massachusetts USAGuangxi Subtropical Crops Research Institute Guangxi Academy of Agricultural Sciences Nanning ChinaTropical Crops Genetic Resources Institute Chinese Academy of Tropical Agricultural Science Haikou ChinaXianghu Laboratory Hangzhou ChinaCollege of Biosystems Engineering and Food Science, Key Laboratory for Agro‐Products Nutritional Evaluation of Ministry of Agriculture and Rural Affairs Zhejiang University Hangzhou ChinaABSTRACT RS4‐type resistant starches have been applied in food industry as thickeners or studied as functional foods for a long time. However, the influence and mechanism of RS4 on Type 2 diabetes mellitus (T2DM) have not been elucidated. In this study, sinapic acid starch ester (SASE) was chosen as a representative of functional RS4, and its anti‐diabetic activity and underlying mechanism were investigated. Results showed that SASE administration lowered blood glucose and ameliorated insulin resistance of streptozotocin‐induced diabetic mice via increasing serum butyrate level. Fecal microbiota transplantation confirmed the T2DM‐improving activity of SASE modulated gut microbiota. 16S rRNA gene sequencing indicated that SASE selectively promoted the proliferation of butyrate‐producing bacterium. In vitro co‐culture of promoted gut bacterium with SASE revealed that Lactobacillus johnsonii was one of the key bacteria in the degradation of SASE. In conclusion, RS4 could selectively promote gut bacteria and increase short‐chain fatty acid production to alleviate T2DM.https://doi.org/10.1002/fft2.528butyrategut microbiotainsulin resistanceLactobacillus johnsoniiresistant starchType 2 diabetes mellitus |
| spellingShingle | Tao Xu Yongheng Zhong Shengyang Ji Hang Xiao Jun Li Kaimian Li Peiwu Li Baiyi Lu RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii Food Frontiers butyrate gut microbiota insulin resistance Lactobacillus johnsonii resistant starch Type 2 diabetes mellitus |
| title | RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii |
| title_full | RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii |
| title_fullStr | RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii |
| title_full_unstemmed | RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii |
| title_short | RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii |
| title_sort | rs4 type resistant starch improves type 2 diabetes mellitus in mice by interacting with lactobacillus johnsonii |
| topic | butyrate gut microbiota insulin resistance Lactobacillus johnsonii resistant starch Type 2 diabetes mellitus |
| url | https://doi.org/10.1002/fft2.528 |
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