Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil

Abstract Microplastics (MPs) in soil are an emerging environmental concern due to their widespread distribution, persistence, potential toxicity to soil organisms, possible transfer into crops and groundwater as well as their potential ability to alter soil functions. MP polymers, such as linear low...

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Main Authors: Eva-Maria Teggers, Svetlana Heck, Boris Meisterjahn, Markus Simon, Dieter Hennecke, Roman Heumann, Holger Egger, Philipp Dalkmann, Annika Jahnke, Andreas Schäffer
Format: Article
Language:English
Published: SpringerOpen 2025-05-01
Series:Microplastics and Nanoplastics
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Online Access:https://doi.org/10.1186/s43591-025-00121-0
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author Eva-Maria Teggers
Svetlana Heck
Boris Meisterjahn
Markus Simon
Dieter Hennecke
Roman Heumann
Holger Egger
Philipp Dalkmann
Annika Jahnke
Andreas Schäffer
author_facet Eva-Maria Teggers
Svetlana Heck
Boris Meisterjahn
Markus Simon
Dieter Hennecke
Roman Heumann
Holger Egger
Philipp Dalkmann
Annika Jahnke
Andreas Schäffer
author_sort Eva-Maria Teggers
collection DOAJ
description Abstract Microplastics (MPs) in soil are an emerging environmental concern due to their widespread distribution, persistence, potential toxicity to soil organisms, possible transfer into crops and groundwater as well as their potential ability to alter soil functions. MP polymers, such as linear low-density polyethylene (LLDPE) and polyurea (PUA) are commonly applied to soils for mulching and pesticide application and their fate needs to be better understood. For qualitative and quantitative analysis, MPs need to be efficiently isolated from soils using suitable extraction methods. Therefore, we developed a modified oil extraction method using n-octanol and compared it with the widely used density extraction method, both preceded by a potassium hydroxide (KOH) extraction step. Pristine and artificially weathered (light-irradiated) small-sized LLDPE mulch film particles (cryo-milled to a median size of 147 µm) and PUA microcapsules used for pesticide applications (median diameter of 2 µm) were spiked into 25 g samples of two standard agricultural soils. Both MP types differed in size, shape, density and especially their composition, as the PUA microcapsules predominantly consisted of oil. After MP isolation, precise MP quantification was facilitated by 14C-radiolabelling of the polymers, which enabled accurate mass balancing while eliminating potential interferences from background polymers. The modified oil extraction method, achieved extraction efficiencies of up to 75% of the applied radioactivity (AR), compared to a maximum efficiency of 62%AR using the conventional density extraction method combined with KOH. Graphical Abstract
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spelling doaj-art-825037cc34b9433d840d9c2085f4a42f2025-08-20T03:48:19ZengSpringerOpenMicroplastics and Nanoplastics2662-49662025-05-015111310.1186/s43591-025-00121-0Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soilEva-Maria Teggers0Svetlana Heck1Boris Meisterjahn2Markus Simon3Dieter Hennecke4Roman Heumann5Holger Egger6Philipp Dalkmann7Annika Jahnke8Andreas Schäffer9INVITE GmbHFraunhofer Institute for Molecular Biology and Applied Ecology IMEFraunhofer Institute for Molecular Biology and Applied Ecology IMEFraunhofer Institute for Molecular Biology and Applied Ecology IMEFraunhofer Institute for Molecular Biology and Applied Ecology IMEINVITE GmbHBayer AG, Research & DevelopmentBayer AG, Research & DevelopmentDepartment of Exposure Science, Helmholtz Centre for Environmental Research - UFZInstitute for Environmental Research, RWTH Aachen UniversityAbstract Microplastics (MPs) in soil are an emerging environmental concern due to their widespread distribution, persistence, potential toxicity to soil organisms, possible transfer into crops and groundwater as well as their potential ability to alter soil functions. MP polymers, such as linear low-density polyethylene (LLDPE) and polyurea (PUA) are commonly applied to soils for mulching and pesticide application and their fate needs to be better understood. For qualitative and quantitative analysis, MPs need to be efficiently isolated from soils using suitable extraction methods. Therefore, we developed a modified oil extraction method using n-octanol and compared it with the widely used density extraction method, both preceded by a potassium hydroxide (KOH) extraction step. Pristine and artificially weathered (light-irradiated) small-sized LLDPE mulch film particles (cryo-milled to a median size of 147 µm) and PUA microcapsules used for pesticide applications (median diameter of 2 µm) were spiked into 25 g samples of two standard agricultural soils. Both MP types differed in size, shape, density and especially their composition, as the PUA microcapsules predominantly consisted of oil. After MP isolation, precise MP quantification was facilitated by 14C-radiolabelling of the polymers, which enabled accurate mass balancing while eliminating potential interferences from background polymers. The modified oil extraction method, achieved extraction efficiencies of up to 75% of the applied radioactivity (AR), compared to a maximum efficiency of 62%AR using the conventional density extraction method combined with KOH. Graphical Abstracthttps://doi.org/10.1186/s43591-025-00121-0MicroplasticsPolyethylenePolyureaMicrocapsulesLight-weatheredExtraction methods
spellingShingle Eva-Maria Teggers
Svetlana Heck
Boris Meisterjahn
Markus Simon
Dieter Hennecke
Roman Heumann
Holger Egger
Philipp Dalkmann
Annika Jahnke
Andreas Schäffer
Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
Microplastics and Nanoplastics
Microplastics
Polyethylene
Polyurea
Microcapsules
Light-weathered
Extraction methods
title Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
title_full Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
title_fullStr Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
title_full_unstemmed Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
title_short Modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
title_sort modified oil extraction of pristine and weathered synthetic polyurea microcapsules and polyethylene microplastics from soil
topic Microplastics
Polyethylene
Polyurea
Microcapsules
Light-weathered
Extraction methods
url https://doi.org/10.1186/s43591-025-00121-0
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