Fast axial resolution enhancement microscopy with structured illumination
In this study, we introduce Fast Axial Resolution Enhancement (FARE) microscopy, which employs tilted strip illumination to achieve a near-isotropic three-dimensional resolution with three images per plane. FARE provides an imaging rate of up to 1.5 Hz for 55 × 55 × 3 μm3 with an axial resolution of...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2024-12-01
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| Series: | APL Photonics |
| Online Access: | http://dx.doi.org/10.1063/5.0239482 |
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| _version_ | 1846093450255007744 |
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| author | Shihang Luo Ran Gong Mingcong Zhang Tao Xu Wei Ji Lusheng Gu |
| author_facet | Shihang Luo Ran Gong Mingcong Zhang Tao Xu Wei Ji Lusheng Gu |
| author_sort | Shihang Luo |
| collection | DOAJ |
| description | In this study, we introduce Fast Axial Resolution Enhancement (FARE) microscopy, which employs tilted strip illumination to achieve a near-isotropic three-dimensional resolution with three images per plane. FARE provides an imaging rate of up to 1.5 Hz for 55 × 55 × 3 μm3 with an axial resolution of ∼270 nm. Using this technique, we observed the movement of the endoplasmic reticulum in three dimensions and discovered novel patterns of mitochondrial morphological transitions and their interactions with other organelles under oxidative stress. These findings highlight the significant potential of FARE microscopy for biomedical applications. |
| format | Article |
| id | doaj-art-81b858ec909848f5a6d43a9e0f11f367 |
| institution | Kabale University |
| issn | 2378-0967 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Photonics |
| spelling | doaj-art-81b858ec909848f5a6d43a9e0f11f3672025-01-02T17:20:58ZengAIP Publishing LLCAPL Photonics2378-09672024-12-01912126116126116-910.1063/5.0239482Fast axial resolution enhancement microscopy with structured illuminationShihang Luo0Ran Gong1Mingcong Zhang2Tao Xu3Wei Ji4Lusheng Gu5Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, ChinaIn this study, we introduce Fast Axial Resolution Enhancement (FARE) microscopy, which employs tilted strip illumination to achieve a near-isotropic three-dimensional resolution with three images per plane. FARE provides an imaging rate of up to 1.5 Hz for 55 × 55 × 3 μm3 with an axial resolution of ∼270 nm. Using this technique, we observed the movement of the endoplasmic reticulum in three dimensions and discovered novel patterns of mitochondrial morphological transitions and their interactions with other organelles under oxidative stress. These findings highlight the significant potential of FARE microscopy for biomedical applications.http://dx.doi.org/10.1063/5.0239482 |
| spellingShingle | Shihang Luo Ran Gong Mingcong Zhang Tao Xu Wei Ji Lusheng Gu Fast axial resolution enhancement microscopy with structured illumination APL Photonics |
| title | Fast axial resolution enhancement microscopy with structured illumination |
| title_full | Fast axial resolution enhancement microscopy with structured illumination |
| title_fullStr | Fast axial resolution enhancement microscopy with structured illumination |
| title_full_unstemmed | Fast axial resolution enhancement microscopy with structured illumination |
| title_short | Fast axial resolution enhancement microscopy with structured illumination |
| title_sort | fast axial resolution enhancement microscopy with structured illumination |
| url | http://dx.doi.org/10.1063/5.0239482 |
| work_keys_str_mv | AT shihangluo fastaxialresolutionenhancementmicroscopywithstructuredillumination AT rangong fastaxialresolutionenhancementmicroscopywithstructuredillumination AT mingcongzhang fastaxialresolutionenhancementmicroscopywithstructuredillumination AT taoxu fastaxialresolutionenhancementmicroscopywithstructuredillumination AT weiji fastaxialresolutionenhancementmicroscopywithstructuredillumination AT lushenggu fastaxialresolutionenhancementmicroscopywithstructuredillumination |