Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3
<i>Priestia megaterium</i> (<i>P. megaterium</i> PH3) is an endophytic bacterium isolated from peanuts. It has natural resveratrol production ability and shows potential application value. This study analyzed its genetic function and metabolic mechanism through whole-genome s...
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MDPI AG
2024-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/22/3555 |
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| author | Xiaohan Zhang Junbo Liang Dong Zhang Liang Wang Shuhong Ye |
| author_facet | Xiaohan Zhang Junbo Liang Dong Zhang Liang Wang Shuhong Ye |
| author_sort | Xiaohan Zhang |
| collection | DOAJ |
| description | <i>Priestia megaterium</i> (<i>P. megaterium</i> PH3) is an endophytic bacterium isolated from peanuts. It has natural resveratrol production ability and shows potential application value. This study analyzed its genetic function and metabolic mechanism through whole-genome sequencing and found that the genome size is 5,960,365 bp, the GC content is 37.62%, and 6132 genes are annotated. Functional analysis showed that this strain contained 149 carbohydrate active enzyme genes, 7 secondary metabolite synthesis gene clusters, 509 virulence genes, and 273 drug-resistance genes. At the same time, this strain has the ability to regulate salt stress, low temperature, and hypoxia. Genomic analysis reveals a stilbene-synthase-containing type III polyketide synthase gene cluster that contributes to resveratrol synthesis. A safety assessment showed that the strain is non-hemolytic, does not produce amino acid decarboxylase, and is not resistant to multiple antibiotics. In the mouse model, <i>P. megaterium</i> PH3 did not have significant effects on body weight, behavior, or physiological indicators. These results provide important basic data and theoretical support for its industrial application and the research and development of plant protection agents. |
| format | Article |
| id | doaj-art-817443afe9b6437b932a3a7a96d3507d |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj-art-817443afe9b6437b932a3a7a96d3507d2024-11-26T18:04:19ZengMDPI AGFoods2304-81582024-11-011322355510.3390/foods13223555Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3Xiaohan Zhang0Junbo Liang1Dong Zhang2Liang Wang3Shuhong Ye4School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaSchool of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, ChinaState Key Laboratory of Marine Food Processing and Safety Control, Dalian 116034, China<i>Priestia megaterium</i> (<i>P. megaterium</i> PH3) is an endophytic bacterium isolated from peanuts. It has natural resveratrol production ability and shows potential application value. This study analyzed its genetic function and metabolic mechanism through whole-genome sequencing and found that the genome size is 5,960,365 bp, the GC content is 37.62%, and 6132 genes are annotated. Functional analysis showed that this strain contained 149 carbohydrate active enzyme genes, 7 secondary metabolite synthesis gene clusters, 509 virulence genes, and 273 drug-resistance genes. At the same time, this strain has the ability to regulate salt stress, low temperature, and hypoxia. Genomic analysis reveals a stilbene-synthase-containing type III polyketide synthase gene cluster that contributes to resveratrol synthesis. A safety assessment showed that the strain is non-hemolytic, does not produce amino acid decarboxylase, and is not resistant to multiple antibiotics. In the mouse model, <i>P. megaterium</i> PH3 did not have significant effects on body weight, behavior, or physiological indicators. These results provide important basic data and theoretical support for its industrial application and the research and development of plant protection agents.https://www.mdpi.com/2304-8158/13/22/3555<i>Priestia megaterium</i>whole genomesafety evaluationtoxicity evaluationbiological characteristics |
| spellingShingle | Xiaohan Zhang Junbo Liang Dong Zhang Liang Wang Shuhong Ye Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 Foods <i>Priestia megaterium</i> whole genome safety evaluation toxicity evaluation biological characteristics |
| title | Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 |
| title_full | Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 |
| title_fullStr | Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 |
| title_full_unstemmed | Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 |
| title_short | Unraveling Whole-Genome Sequence and Functional Characterization of <i>P. megaterium</i> PH3 |
| title_sort | unraveling whole genome sequence and functional characterization of i p megaterium i ph3 |
| topic | <i>Priestia megaterium</i> whole genome safety evaluation toxicity evaluation biological characteristics |
| url | https://www.mdpi.com/2304-8158/13/22/3555 |
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