Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya
Abstract Root-knot nematodes (Meloidogyne spp.) are significant pests that cause considerable damage to crops, prompting a need for sustainable control methods. This study evaluated the nematicidal potential of fungal culture filtrates and botanicals as eco-friendly alternatives. In vitro tests demo...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84292-5 |
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| author | H. Jeevan Raghubir Kumar Patidar Veronica Kadam Pranab Dutta Euphema Nongbri M. N. Rudra Gouda Santosh Naik N. S Nysanth |
| author_facet | H. Jeevan Raghubir Kumar Patidar Veronica Kadam Pranab Dutta Euphema Nongbri M. N. Rudra Gouda Santosh Naik N. S Nysanth |
| author_sort | H. Jeevan |
| collection | DOAJ |
| description | Abstract Root-knot nematodes (Meloidogyne spp.) are significant pests that cause considerable damage to crops, prompting a need for sustainable control methods. This study evaluated the nematicidal potential of fungal culture filtrates and botanicals as eco-friendly alternatives. In vitro tests demonstrated that Lemongrass oil (LG) (0.2%) achieved the highest mortality of nematode juveniles (J2s) at 99.44% within 48 h, while Pochonia chlamydosporia (Pc) (2%) and Purpuricillium lilacinum (Pl) (2%) reduced egg hatching rates to 9.57% and 11.43%, respectively. Neem oil (NM) (0.2%) was the most effective in preventing J2 root penetration (4.42%). In vivo, a combination treatment (T7) of NM (0.2%), Trichoderma harzianum (Tz) (2%), Pl (2%), and LG (0.2%) applied at 10 day intervals significantly reduced the nematode reproduction factor to 0.035, comparable to the chemical control Bayer Velum Prime® (Fluopyram 34.48% W/W SC) (0.031). T5 (NM and Tz) resulted in the highest shoot biomass (236.73 ± 1.38 g), while Bayer Velum Prime® (Fluopyram 34.48% W/W SC) increased root biomass (31.75 ± 1.24 g). Additionally, T7 produced the longest shoots (63.37 ± 0.74 cm) and roots (36.80 ± 0.3 cm), with fewer root galls (55.67 ± 1.53) and egg masses (4 ± 0.01). T7 also minimized the final soil nematode population to 106.33 ± 1.01 per 100 g, closely followed by T8 (94.67 ± 0.89). These results indicate that combining NM, Tz, Pl and LG provide effective nematode control without phytotoxic effects, enhancing plant growth and offering a promising sustainable alternative to chemical nematicides. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-7949eddc1f344fb3be28852f975aa9982025-01-05T12:13:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-84292-5Implementing sustainable practices to combat root knot nematode infestation in tomato farming from MeghalayaH. Jeevan0Raghubir Kumar Patidar1Veronica Kadam2Pranab Dutta3Euphema Nongbri4M. N. Rudra Gouda5Santosh Naik6N. S Nysanth7Division of Nematology, Indian Agricultural Research InstituteSchool of Crop Protection, Nematology, Collage of Post Graduate Studies in Agricultural Sciences, CAUSchool of Crop Protection, Nematology, Collage of Post Graduate Studies in Agricultural Sciences, CAUPlant (Pathology), CoA, CAU-ImphalSchool of Crop Protection, Nematology, Collage of Post Graduate Studies in Agricultural Sciences, CAUDivision of Entomology, Indian Agricultural Research InstituteDivision of Entomology, Indian Agricultural Research InstituteDivision of Microbiology, Indian Agricultural Research InstituteAbstract Root-knot nematodes (Meloidogyne spp.) are significant pests that cause considerable damage to crops, prompting a need for sustainable control methods. This study evaluated the nematicidal potential of fungal culture filtrates and botanicals as eco-friendly alternatives. In vitro tests demonstrated that Lemongrass oil (LG) (0.2%) achieved the highest mortality of nematode juveniles (J2s) at 99.44% within 48 h, while Pochonia chlamydosporia (Pc) (2%) and Purpuricillium lilacinum (Pl) (2%) reduced egg hatching rates to 9.57% and 11.43%, respectively. Neem oil (NM) (0.2%) was the most effective in preventing J2 root penetration (4.42%). In vivo, a combination treatment (T7) of NM (0.2%), Trichoderma harzianum (Tz) (2%), Pl (2%), and LG (0.2%) applied at 10 day intervals significantly reduced the nematode reproduction factor to 0.035, comparable to the chemical control Bayer Velum Prime® (Fluopyram 34.48% W/W SC) (0.031). T5 (NM and Tz) resulted in the highest shoot biomass (236.73 ± 1.38 g), while Bayer Velum Prime® (Fluopyram 34.48% W/W SC) increased root biomass (31.75 ± 1.24 g). Additionally, T7 produced the longest shoots (63.37 ± 0.74 cm) and roots (36.80 ± 0.3 cm), with fewer root galls (55.67 ± 1.53) and egg masses (4 ± 0.01). T7 also minimized the final soil nematode population to 106.33 ± 1.01 per 100 g, closely followed by T8 (94.67 ± 0.89). These results indicate that combining NM, Tz, Pl and LG provide effective nematode control without phytotoxic effects, enhancing plant growth and offering a promising sustainable alternative to chemical nematicides.https://doi.org/10.1038/s41598-024-84292-5Root knot nematodeBotanicalsBiocontrol agentsEnvironmentally friendly |
| spellingShingle | H. Jeevan Raghubir Kumar Patidar Veronica Kadam Pranab Dutta Euphema Nongbri M. N. Rudra Gouda Santosh Naik N. S Nysanth Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya Scientific Reports Root knot nematode Botanicals Biocontrol agents Environmentally friendly |
| title | Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya |
| title_full | Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya |
| title_fullStr | Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya |
| title_full_unstemmed | Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya |
| title_short | Implementing sustainable practices to combat root knot nematode infestation in tomato farming from Meghalaya |
| title_sort | implementing sustainable practices to combat root knot nematode infestation in tomato farming from meghalaya |
| topic | Root knot nematode Botanicals Biocontrol agents Environmentally friendly |
| url | https://doi.org/10.1038/s41598-024-84292-5 |
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