Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts
Optical imaging has made it possible to monitor response to anticancer therapies in tumor xenografts. The concept of treating breast cancers with 131 I is predicated on the expression of the Na + /I − symporter (NIS) in many tumors and uptake of I in some. The pattern of 131 I radioablative effects...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2006-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2006.00008 |
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| _version_ | 1846097908090273792 |
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| author | Malavika Ghosh Sanjiv Sam Gambhir Abhijit De Kent Nowels Michael Goris Irene Wapnir |
| author_facet | Malavika Ghosh Sanjiv Sam Gambhir Abhijit De Kent Nowels Michael Goris Irene Wapnir |
| author_sort | Malavika Ghosh |
| collection | DOAJ |
| description | Optical imaging has made it possible to monitor response to anticancer therapies in tumor xenografts. The concept of treating breast cancers with 131 I is predicated on the expression of the Na + /I − symporter (NIS) in many tumors and uptake of I in some. The pattern of 131 I radioablative effects were investigated in an MCF-7 xenograft model dually transfected with firefly luciferase and NIS genes. On Day 16 after tumor cell implantation, 3 mCi of 131 I was injected. Bioluminescent imaging using d -luciferin and a cooled charge-coupled device camera was carried out on Days 1, 2, 3, 7, 10, 16, 22, 29, and 35. Tumor bioluminescence decreased in 131 I-treated tumors after Day 3 and reached a nadir on Day 22. Conversely, bioluminescence steadily increased in controls and was 3.85-fold higher than in treated tumors on Day 22. Bioluminescence in 131 I-treated tumors increased after Day 22, corresponding to tumor regrowth. By Day 35, treated tumors were smaller and accumulated 33% less 99m TcO 4 than untreated tumors. NIS immunoreactivity was present in <50% of 131 I-treated cells compared to 85–90% of controls. In summary, a pattern of tumor regression occurring over the first three weeks after 131 I administration was observed in NIS-expressing breast cancer xenografts. |
| format | Article |
| id | doaj-art-78badc48ed3b41e7a2cb78a2c07c33d2 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2006-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-78badc48ed3b41e7a2cb78a2c07c33d22025-01-02T02:58:10ZengSAGE PublishingMolecular Imaging1536-01212006-04-01510.2310/7290.2006.0000810.2310_7290.2006.00008Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 XenograftsMalavika GhoshSanjiv Sam GambhirAbhijit DeKent NowelsMichael GorisIrene WapnirOptical imaging has made it possible to monitor response to anticancer therapies in tumor xenografts. The concept of treating breast cancers with 131 I is predicated on the expression of the Na + /I − symporter (NIS) in many tumors and uptake of I in some. The pattern of 131 I radioablative effects were investigated in an MCF-7 xenograft model dually transfected with firefly luciferase and NIS genes. On Day 16 after tumor cell implantation, 3 mCi of 131 I was injected. Bioluminescent imaging using d -luciferin and a cooled charge-coupled device camera was carried out on Days 1, 2, 3, 7, 10, 16, 22, 29, and 35. Tumor bioluminescence decreased in 131 I-treated tumors after Day 3 and reached a nadir on Day 22. Conversely, bioluminescence steadily increased in controls and was 3.85-fold higher than in treated tumors on Day 22. Bioluminescence in 131 I-treated tumors increased after Day 22, corresponding to tumor regrowth. By Day 35, treated tumors were smaller and accumulated 33% less 99m TcO 4 than untreated tumors. NIS immunoreactivity was present in <50% of 131 I-treated cells compared to 85–90% of controls. In summary, a pattern of tumor regression occurring over the first three weeks after 131 I administration was observed in NIS-expressing breast cancer xenografts.https://doi.org/10.2310/7290.2006.00008 |
| spellingShingle | Malavika Ghosh Sanjiv Sam Gambhir Abhijit De Kent Nowels Michael Goris Irene Wapnir Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts Molecular Imaging |
| title | Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts |
| title_full | Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts |
| title_fullStr | Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts |
| title_full_unstemmed | Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts |
| title_short | Bioluminescent Monitoring of NIS-Mediated I Ablative Effects in MCF-7 Xenografts |
| title_sort | bioluminescent monitoring of nis mediated i ablative effects in mcf 7 xenografts |
| url | https://doi.org/10.2310/7290.2006.00008 |
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