Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord
Introduction: Oxaliplatin is a platinum-based chemotherapy agent that often causes chemotherapy-induced peripheral neuropathy (CIPN). This effect limits the potential activity and decreases the cancer patient’s quality of life. Melanocortin and transient receptor potential ankyrin 1 (TRPA1) pathways...
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| Language: | English |
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Belgorod National Research University
2024-09-01
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| Series: | Research Results in Pharmacology |
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| Online Access: | https://rrpharmacology.ru/index.php/journal/article/view/478 |
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| author | Alma Nuril Aliya Luke Wongso Diah Ayu Wakita Trimanda Risda Maulida I Nengah Budi Sumartha Samirah Samirah Abdulloh Machin Long Chiau Ming Abdelhakim Bouyahya Mahardian Rahmadi Junaidi Khotib Сhrismawan Ardianto |
| author_facet | Alma Nuril Aliya Luke Wongso Diah Ayu Wakita Trimanda Risda Maulida I Nengah Budi Sumartha Samirah Samirah Abdulloh Machin Long Chiau Ming Abdelhakim Bouyahya Mahardian Rahmadi Junaidi Khotib Сhrismawan Ardianto |
| author_sort | Alma Nuril Aliya |
| collection | DOAJ |
| description | Introduction: Oxaliplatin is a platinum-based chemotherapy agent that often causes chemotherapy-induced peripheral neuropathy (CIPN). This effect limits the potential activity and decreases the cancer patient’s quality of life. Melanocortin and transient receptor potential ankyrin 1 (TRPA1) pathways are believed to be essential in recruiting an allodynia response. Based on previous studies, curcumin has shown antioxidant and anti-inflammatory activities that could potentially be useful for decreasing the allodynia effects of oxaliplatin treatment. This study investigated the effect of curcumin on CIPN conditions. In addition, we further elaborated to measure the involvement of spinal melanocortin and the TRPA1 system.
Materials and Methods: A total of 30 male Balb/C mice aged 6-7 weeks old and weighing 20-30 g were used in this study. Mice were injected with oxaliplatin 3 mg/kg four times in the first week of the study. In the second week of the study, curcumin 30, 60, and 120 mg/kg was injected intraperitoneally for 7 days. Allodynia response was measured using the von Frey filament test. Melanocortin 4 receptor (Mc4r), Pro-opiomelanocortin (Pomc) and Trpa1 mRNA expressions were measured using RT-qPCR.
Results: Oxaliplatin-induced mechanical allodynia response in mice, characterized by a decrease in the 50% withdrawal threshold parameter, was followed by a significant decrease in the Mc4r, Pomc, and Trpa1 mRNA expressions in the spinal cord. Curcumin administration in all doses improves the 50% withdrawal threshold parameter in mice induced by oxaliplatin. Furthermore, curcumin increases the Mc4r and Pomc, but not the Trpa1 mRNA expressions in the spinal cord.
Conclusion: Curcumin significantly reduces the allodynia response induced by oxaliplatin. In addition, curcumin ameliorates the melanocortin, but not TRPA1, downregulation in the spinal cord. |
| format | Article |
| id | doaj-art-7796ceb5054a4d7184885cd5cd480832 |
| institution | Kabale University |
| issn | 2658-381X |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Belgorod National Research University |
| record_format | Article |
| series | Research Results in Pharmacology |
| spelling | doaj-art-7796ceb5054a4d7184885cd5cd4808322025-01-08T12:30:29ZengBelgorod National Research UniversityResearch Results in Pharmacology2658-381X2024-09-01103535910.18413/rrpharmacology.10.478Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cordAlma Nuril Aliya0https://orcid.org/0009-0009-8990-5570Luke Wongso1https://orcid.org/0009-0008-0002-181XDiah Ayu Wakita Trimanda 2https://orcid.org/0009-0007-1057-1391Risda Maulida3https://orcid.org/0009-0003-1859-2278I Nengah Budi Sumartha4https://orcid.org/0009-0008-2509-6930Samirah Samirah5https://orcid.org/0000-0002-7918-5487Abdulloh Machin6https://orcid.org/0000-0003-0369-0898Long Chiau Ming7https://orcid.org/0000-0002-6971-1383Abdelhakim Bouyahya8https://orcid.org/0000-0001-9317-1631Mahardian Rahmadi9https://orcid.org/0000-0002-7256-2446Junaidi Khotib10https://orcid.org/0000-0002-8468-8441Сhrismawan Ardianto11Universitas Airlangga, IndonesiaUniversitas Airlangga, Surabaya, IndonesiaUniversitas Airlangga, Indonesia Universitas Airlangga, Surabaya, IndonesiaUniversitas Airlangga, IndonesiaUniversitas Airlangga, Surabaya, IndonesiaUniversitas Airlangga, Surabaya, IndonesiaSunway University, MalaysiaMohammed V University in Rabat, MoroccoUniversitas Airlangga, IndonesiaUniversitas Airlangga, IndonesiaUniversitas Airlangga, SurabayaIntroduction: Oxaliplatin is a platinum-based chemotherapy agent that often causes chemotherapy-induced peripheral neuropathy (CIPN). This effect limits the potential activity and decreases the cancer patient’s quality of life. Melanocortin and transient receptor potential ankyrin 1 (TRPA1) pathways are believed to be essential in recruiting an allodynia response. Based on previous studies, curcumin has shown antioxidant and anti-inflammatory activities that could potentially be useful for decreasing the allodynia effects of oxaliplatin treatment. This study investigated the effect of curcumin on CIPN conditions. In addition, we further elaborated to measure the involvement of spinal melanocortin and the TRPA1 system. Materials and Methods: A total of 30 male Balb/C mice aged 6-7 weeks old and weighing 20-30 g were used in this study. Mice were injected with oxaliplatin 3 mg/kg four times in the first week of the study. In the second week of the study, curcumin 30, 60, and 120 mg/kg was injected intraperitoneally for 7 days. Allodynia response was measured using the von Frey filament test. Melanocortin 4 receptor (Mc4r), Pro-opiomelanocortin (Pomc) and Trpa1 mRNA expressions were measured using RT-qPCR. Results: Oxaliplatin-induced mechanical allodynia response in mice, characterized by a decrease in the 50% withdrawal threshold parameter, was followed by a significant decrease in the Mc4r, Pomc, and Trpa1 mRNA expressions in the spinal cord. Curcumin administration in all doses improves the 50% withdrawal threshold parameter in mice induced by oxaliplatin. Furthermore, curcumin increases the Mc4r and Pomc, but not the Trpa1 mRNA expressions in the spinal cord. Conclusion: Curcumin significantly reduces the allodynia response induced by oxaliplatin. In addition, curcumin ameliorates the melanocortin, but not TRPA1, downregulation in the spinal cord.https://rrpharmacology.ru/index.php/journal/article/view/478allodyniacancercipncurcuminmelanocortinneuropathyoxaliplatintrpa1 |
| spellingShingle | Alma Nuril Aliya Luke Wongso Diah Ayu Wakita Trimanda Risda Maulida I Nengah Budi Sumartha Samirah Samirah Abdulloh Machin Long Chiau Ming Abdelhakim Bouyahya Mahardian Rahmadi Junaidi Khotib Сhrismawan Ardianto Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord Research Results in Pharmacology allodynia cancer cipn curcumin melanocortin neuropathy oxaliplatin trpa1 |
| title | Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord |
| title_full | Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord |
| title_fullStr | Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord |
| title_full_unstemmed | Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord |
| title_short | Curcumin ameliorates oxaliplatin-induced allodynia response and melanocortin downregulation in the spinal cord |
| title_sort | curcumin ameliorates oxaliplatin induced allodynia response and melanocortin downregulation in the spinal cord |
| topic | allodynia cancer cipn curcumin melanocortin neuropathy oxaliplatin trpa1 |
| url | https://rrpharmacology.ru/index.php/journal/article/view/478 |
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