Oxidative stress promotes lipid-laden macrophage formation via CYP1B1
Emerging evidence suggests that lipid-laden macrophages (LLM) participate in lung damage in various clinical conditions. However, the mechanisms involved in LLM formation are not fully understood. In this study, we aimed to investigate the link between reactive oxygen species (ROS) and LLM formation...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Redox Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213231724004592 |
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| author | Yin Zhu Saugata Dutta Yohan Han Dooyoung Choi Francesca Polverino Caroline A. Owen Payaningal R. Somanath Xiaoyun Wang Duo Zhang |
| author_facet | Yin Zhu Saugata Dutta Yohan Han Dooyoung Choi Francesca Polverino Caroline A. Owen Payaningal R. Somanath Xiaoyun Wang Duo Zhang |
| author_sort | Yin Zhu |
| collection | DOAJ |
| description | Emerging evidence suggests that lipid-laden macrophages (LLM) participate in lung damage in various clinical conditions. However, the mechanisms involved in LLM formation are not fully understood. In this study, we aimed to investigate the link between reactive oxygen species (ROS) and LLM formation. We found that ROS triggered by cigarette smoke extract (CSE) or H2O2 significantly promoted LLM formation. Given the key role of ROS in LLM formation, we further demonstrated that LLM formation is induced by various ROS-producing stimuli, including bacteria, oxidized low-density lipoprotein (OxLDL), hyperoxia, and E-cigarette vapor extract (EVE). Meanwhile, cytochrome P450 family-1 subfamily B member 1 (CYP1B1) was highly upregulated in lung macrophages from chronic obstructive pulmonary disease (COPD) patients and CSE-treated macrophages. Functionally, CYP1B1 contributes to the CSE-induced lipid accumulation and LLM formation. CYP1B1 expression and LLM formation were effectively suppressed by antioxidant N-acetylcysteine (NAC) and carvedilol. The formation of LLM was also associated with classically activated M1 but not the M2 state. CSE-induced LLM showed time-dependent alterations in inflammatory response and phagocytic ability. In summary, our study highlights the role of oxidative stress in LLM formation. CYP1B1 contributes to ROS-induced LLM formation and may serve as a therapeutic target for reducing LLM-induced lung damage. |
| format | Article |
| id | doaj-art-76105eb70cd04a26aa513eed819f8d00 |
| institution | Kabale University |
| issn | 2213-2317 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Redox Biology |
| spelling | doaj-art-76105eb70cd04a26aa513eed819f8d002025-01-14T04:12:13ZengElsevierRedox Biology2213-23172025-02-0179103481Oxidative stress promotes lipid-laden macrophage formation via CYP1B1Yin Zhu0Saugata Dutta1Yohan Han2Dooyoung Choi3Francesca Polverino4Caroline A. Owen5Payaningal R. Somanath6Xiaoyun Wang7Duo Zhang8Clinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Charlie Norwood VA Medical Center, Augusta, GA, 30912, USAClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Charlie Norwood VA Medical Center, Augusta, GA, 30912, USAClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Department of Microbiology, Wonkwang University School of Medicine, Iksan, 54538, Republic of KoreaClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USABaylor College of Medicine, Medicine, Houston, TX, 77030, USADivision of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USAClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Charlie Norwood VA Medical Center, Augusta, GA, 30912, USAClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Charlie Norwood VA Medical Center, Augusta, GA, 30912, USA; Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, 02115, USAClinical and Experimental Therapeutics, College of Pharmacy, University of Georgia, Augusta, GA, 30912, USA; Charlie Norwood VA Medical Center, Augusta, GA, 30912, USA; Department of Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA; Corresponding author. 914 New Bailie Street, Augusta, GA, 30901, USA.Emerging evidence suggests that lipid-laden macrophages (LLM) participate in lung damage in various clinical conditions. However, the mechanisms involved in LLM formation are not fully understood. In this study, we aimed to investigate the link between reactive oxygen species (ROS) and LLM formation. We found that ROS triggered by cigarette smoke extract (CSE) or H2O2 significantly promoted LLM formation. Given the key role of ROS in LLM formation, we further demonstrated that LLM formation is induced by various ROS-producing stimuli, including bacteria, oxidized low-density lipoprotein (OxLDL), hyperoxia, and E-cigarette vapor extract (EVE). Meanwhile, cytochrome P450 family-1 subfamily B member 1 (CYP1B1) was highly upregulated in lung macrophages from chronic obstructive pulmonary disease (COPD) patients and CSE-treated macrophages. Functionally, CYP1B1 contributes to the CSE-induced lipid accumulation and LLM formation. CYP1B1 expression and LLM formation were effectively suppressed by antioxidant N-acetylcysteine (NAC) and carvedilol. The formation of LLM was also associated with classically activated M1 but not the M2 state. CSE-induced LLM showed time-dependent alterations in inflammatory response and phagocytic ability. In summary, our study highlights the role of oxidative stress in LLM formation. CYP1B1 contributes to ROS-induced LLM formation and may serve as a therapeutic target for reducing LLM-induced lung damage.http://www.sciencedirect.com/science/article/pii/S2213231724004592CYP1B1COPDCigarette smokingE-CigaretteReactive oxygen speciesCarvedilol |
| spellingShingle | Yin Zhu Saugata Dutta Yohan Han Dooyoung Choi Francesca Polverino Caroline A. Owen Payaningal R. Somanath Xiaoyun Wang Duo Zhang Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 Redox Biology CYP1B1 COPD Cigarette smoking E-Cigarette Reactive oxygen species Carvedilol |
| title | Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 |
| title_full | Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 |
| title_fullStr | Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 |
| title_full_unstemmed | Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 |
| title_short | Oxidative stress promotes lipid-laden macrophage formation via CYP1B1 |
| title_sort | oxidative stress promotes lipid laden macrophage formation via cyp1b1 |
| topic | CYP1B1 COPD Cigarette smoking E-Cigarette Reactive oxygen species Carvedilol |
| url | http://www.sciencedirect.com/science/article/pii/S2213231724004592 |
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